Babco, Signet, Sternberger Monoclonals, Senetek, Covance

ELISA/assay

Bromodeoxyuridine (BrdU)

423401

25 mg

72 USD

423401

Bromodeoxyuridine (BrdU)

5-Bromo-2'-Deoxyuridine, BUDR , 5-BrdU, 5-Bromouracil deoxyriboside, 5-Bromo-1-(2-deoxy-beta-D-ribofuranosyl)uracil

25 mg

72 USD

NONAB_CHMPRB

This product can be used for in vivo and in vitro labeling of proliferating cells as described in the following protocols: a) Administration in drinking water: Dilute the BrdU in drinking water to a concentration of 0.8-1.0 mg/ml. Give to animals for consumption ad libitum for up to 4 weeks. Note: This solution should be protected from light and should be refreshed at least every 3 days. BrdU can be toxic, even lethal. If animals are going to be fed with BrdU for more than 2 weeks, then closely monitor the animals for signs of toxicity. b) Administration by injection: Dilute the BrdU in sterile saline solution or PBS for a dose of 50-100 mg BrdU/kg, inject the animal intraperitoneally (i.p.) or intravenously (i.v.). In actively proliferating cells, BrdU-labeled DNA can be detected within one hour of the injection. c) In vitro administration of BrdU: Dilute the BrdU 1:30 (0.33 mg/ml) with sterile media and pulse the cells by adding 10 ul of this solution to each ml of cultured cells, BrdU final concentration is 10 uM (3.3 ug/ml). A cell density =2x10 6 cells/ml is commonly used; incubate the cells for desired time. Note: Special care should be taken to not disturb the cells before pulsing them with BrdU and during the incubation time (e.g. avoid changes in temperature and do not centrifuge the cells), as it may interfere with the cell cycle. The protocols above outline three recommended uses for this product. Final conditions will depend on the purpose of the experiment and characteristics of the target cells.