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product summary
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
ELISA/assay
product name :
Cell Activation Cocktail (with Brefeldin A)
catalog :
423304
quantity :
400 μL
price :
314 USD
citations: 29
Reference
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Li D, Bradley T, Cain D, Pedroza Pacheco I, Aggelakopoulou M, Parks R, et al. RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures. Immunohorizons. 2020;4:713-728 pubmed publisher
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Lin H, Wang Q, Liu L, Chen Z, Das R, Zhao Y, et al. Colonization of Mice With Amoxicillin-Associated Klebsiella variicola Drives Inflammation via Th1 Induction and Treg Inhibition. Front Microbiol. 2020;11:1256 pubmed publisher
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product information
Apps. Abbrev. :
Activ, Flow Cytometry, Intracellular Staining for Flow Cytometry
Cat # :
423304
Item :
Cell Activation Cocktail (with Brefeldin A)
Other Names :
Cell Stimulation Cocktail, PMA/Ionomycin
Size :
400 μL
Price (USD) :
314 USD
Conjugate/Tag/Label :
COCKTAIL
Application Notes :
Cell Activation Protocol: 1. Resuspend cells of interest in desired cell culture medium. Recommended cell suspension is between 1-2 x 10 6 cells/mL, although effective activation can be obtained with higher or lower concentration. 2. Completely thaw vials in 37°C water bath. 3. Add 2 µL of the cocktail to each mL of cell suspension. 4. Incubate cells at 37°C in a CO2 incubator for 6 hours or the time period of interest. 5. Harvest activated cells and centrifuge at 350g for 5 minutes. Discard supernatant. 6. Add 2.5 mL of Cell Staining Buffer (Cat. No. 420201) to the cell pellet, mix by vortex or pipetting, then centrifuge at 350g for 5 minutes. Discard supernatant. 7. Repeat step 6. 8. Cells are now ready to be tested for surface markers or proteins of interest. Note: Activation of cells overnight ( 12 hours) with Brefeldin A is generally not recommended due to some level of toxicity. Under these conditions, it is recommended to use Cell Activation Cocktail without Brefeldin A (Cat. No. 423301), and then add monensin (Cat. No. 420701) at appropriate concentrations. This product is temperature sensitive; repeated freeze/thaw is not recommended. Intracellular and surface flow staining, as well as ELISA, can be performed following activation of cells with this cocktail.
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA