Cell Activation Cocktail (without Brefeldin A) | BioLegend 423302 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

ELISA/assay

product name :

Cell Activation Cocktail (without Brefeldin A)

catalog :

423302

quantity :

400 μL

price :

248 USD

citations: 9

Reference
Stephenson E, Reynolds G, Botting R, Calero Nieto F, Morgan M, Tuong Z, et al. Single-cell multi-omics analysis of the immune response in COVID-19. Nat Med. 2021;27:904-916 pubmed publisher
Lee J, Sheen J, Lim O, Lee Y, Ryu J, Shin D, et al. Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy. Sci Rep. 2020;10:17753 pubmed publisher
Vazquez J, Chasman D, Lopez G, Tyler C, Ong I, Stanic A. Transcriptional and Functional Programming of Decidual Innate Lymphoid Cells. Front Immunol. 2019;10:3065 pubmed publisher
Carpenter R, Jiang R, Brennan F, Hall J, Gottipati M, Niewiesk S, et al. Human immune cells infiltrate the spinal cord and impair recovery after spinal cord injury in humanized mice. Sci Rep. 2019;9:19105 pubmed publisher
Owyong M, Chou J, van den Bijgaart R, Kong N, Efe G, Maynard C, et al. MMP9 modulates the metastatic cascade and immune landscape for breast cancer anti-metastatic therapy. Life Sci Alliance. 2019;2: pubmed publisher
Chen Y, Wang Y, Lei C, Changou C, Davis M, Yen Y. Host immune response to anti-cancer camptothecin conjugated cyclodextrin-based polymers. J Biomed Sci. 2019;26:85 pubmed publisher
Qi X, Yun C, Sun L, Xia J, Wu Q, Wang Y, et al. Gut microbiota-bile acid-interleukin-22 axis orchestrates polycystic ovary syndrome. Nat Med. 2019;25:1225-1233 pubmed publisher
Cella M, Gamini R, Sécca C, Collins P, Zhao S, Peng V, et al. Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues. Nat Immunol. 2019;: pubmed publisher
Mohammadi Ayenehdeh J, Niknam B, Rasouli S, Hashemi S, Rahavi H, Rezaei N, et al. Immunomodulatory and protective effects of adipose tissue-derived mesenchymal stem cells in an allograft islet composite transplantation for experimental autoimmune type 1 diabetes. Immunol Lett. 2017;188:21-31 pubmed publisher

product information

Apps. Abbrev. :

Activ, Flow Cytometry, Intracellular Staining for Flow Cytometry

Cat # :

423302

Item :

Cell Activation Cocktail (without Brefeldin A)

Other Names :

Cell Stimulation Cocktail, PMA/Ionomycin

Size :

400 μL

Price (USD) :

248 USD

Conjugate/Tag/Label :

COCKTAIL

Application Notes :

Cell Activation Protocol: 1. Resuspend cells of interest in desired cell culture medium. Recommended cell suspension is between 1-2 x 10 6 cells/mL, although effective activation can be obtained with higher or lower concentration. 2. Completely thaw vials in 37 C water bath. 3. Add 2 uL of the cocktail to each mL of cell suspension. 4. Incubate cells at 37 C in a CO 2 incubator for 6 hours or the time period of interest. 5. Harvest activated cells and centrifuge at 350g for 5 minutes. Discard supernatant. 6. Add 2.5 mL of Cell Staining Buffer (Cat. No. 420201) to the cell pellet, mix by vortex or pipetting, then centrifuge at 350g for 5 minutes. Discard supernatant. 7. Repeat step 6. 8. Cells are now ready to be tested for surface markers or proteins of interest. Note: If investigators are interested in detecting intracellular proteins following activation, this product can be used in conjunction with Monensin (Cat. No. 420701) or Brefeldin A (Cat. No. 420601). This product is temperature sensitive; repeated freeze/thaw is not recommended. Intracellular and surface flow staining, as well as ELISA, can be performed following activation of cells with this cocktail.