Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Zombie UV387™ Fixable Viability Kit

423126

500 tests

335 USD

423126

Zombie UV387™ Fixable Viability Kit

500 tests

335 USD

NONAB_CHMPRB

Zombie UV387™ dye is excited by the UV laser (355 nm) and has fluorescence emission maximum at 387 nm. If using in a multi-color panel design, filter optimization may be required depending on other fluorophores used. Zombie UV387™ has similar spectra to Spark UV™ 387.Standard Cell Staining Protocol:;Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial.;For reconstitution, pre-warm the kit to room temperature; add 100 µL of DMSO to one vial of Zombie UV387™ dye and mix until fully dissolved.;Wash cells with PBS buffer (no Tris buffer and protein free).;Dilute Zombie UV387™ dye at 1:100 - 1000 in PBS. Resuspend 1-10 x 10^6 cells in diluted 100 µL Zombie UV387™ solution. To minimize background staining of live cells, titrate the amount of dye and/or number of cells per 100 µL for optimal performance. Different cell types can have a wide degree of variability in staining based on cell size and degree of cell death.Note: Don't use Tris buffer as a diluent and be sure that the PBS does not contain any other protein like BSA or FBS.;Note: The amount of dye used can also influence the ability to detect apoptotic as well as live and dead cells.;Incubate the cells at room temperature (or 4°C), in the dark, for 15 - 30 minutes.;Wash one time with 2 mL BioLegend's Cell Staining Buffer (Cat. No. 420201) or equivalent buffer containing serum or BSA.;Continue performing antibody staining procedure as desired.;Cells can be fixed with paraformaldehyde or methanol prior to permeabilization or can be analyzed without fixation.;