Zombie Green™ Fixable Viability Kit | BioLegend 423112 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

ELISA/assay

product name :

Zombie Green™ Fixable Viability Kit

catalog :

423112

quantity :

500 tests

price :

314 USD

citations: 17

Reference
Fabian P, Tseng K, Thiruppathy M, Arata C, Chen H, Smeeton J, et al. Lifelong single-cell profiling of cranial neural crest diversification in zebrafish. Nat Commun. 2022;13:13 pubmed publisher
Lago Alvarez Y, Podico G, Segabinazzi L, Cunha L, Barbosa L, Arnold C, et al. Donkey Epididymal Transport for Semen Cooling and Freezing. Animals (Basel). 2020;10: pubmed publisher
Meyrath M, Szpakowska M, Zeiner J, Massotte L, Merz M, Benkel T, et al. The atypical chemokine receptor ACKR3/CXCR7 is a broad-spectrum scavenger for opioid peptides. Nat Commun. 2020;11:3033 pubmed publisher
Ireland A, Micinski A, Kastner D, Guo B, Wait S, Spainhower K, et al. MYC Drives Temporal Evolution of Small Cell Lung Cancer Subtypes by Reprogramming Neuroendocrine Fate. Cancer Cell. 2020;38:60-78.e12 pubmed publisher
Donado C, Cao A, Simmons D, Croker B, Brennan P, Brenner M. A Two-Cell Model for IL-1β Release Mediated by Death-Receptor Signaling. Cell Rep. 2020;31:107466 pubmed publisher
Cho S, Chae J, Shin H, Shin Y, Kim Y, Kil E, et al. Enhanced Anticancer Effect of Adding Magnesium to Vitamin C Therapy: Inhibition of Hormetic Response by SVCT-2 Activation. Transl Oncol. 2020;13:401-409 pubmed publisher
Zheng W, Zhang H, Zhao D, Zhang J, Pollard J. Lung Mammary Metastases but Not Primary Tumors Induce Accumulation of Atypical Large Platelets and Their Chemokine Expression. Cell Rep. 2019;29:1747-1755.e4 pubmed publisher
Orozco S, Daniels B, Yatim N, Messmer M, Quarato G, Chen Harris H, et al. RIPK3 Activation Leads to Cytokine Synthesis that Continues after Loss of Cell Membrane Integrity. Cell Rep. 2019;28:2275-2287.e5 pubmed publisher
Rubin S, Bai L, Haileselassie Y, Garay G, Yun C, Becker L, et al. Mass cytometry reveals systemic and local immune signatures that distinguish inflammatory bowel diseases. Nat Commun. 2019;10:2686 pubmed publisher
Freemerman A, Zhao L, Pingili A, Teng B, Cozzo A, Fuller A, et al. Myeloid Slc2a1-Deficient Murine Model Revealed Macrophage Activation and Metabolic Phenotype Are Fueled by GLUT1. J Immunol. 2019;202:1265-1286 pubmed publisher
Sivick K, Desbien A, Glickman L, Reiner G, Corrales L, Surh N, et al. Magnitude of Therapeutic STING Activation Determines CD8+ T Cell-Mediated Anti-tumor Immunity. Cell Rep. 2018;25:3074-3085.e5 pubmed publisher
Muire P, Hanson L, Wills R, Petrie Hanson L. Differential gene expression following TLR stimulation in rag1-/- mutant zebrafish tissues and morphological descriptions of lymphocyte-like cell populations. PLoS ONE. 2017;12:e0184077 pubmed publisher
Zufferey R, Pirani K, Cheung See Kit M, Lee S, Williams T, Chen D, et al. The Trypanosoma brucei dihydroxyacetonephosphate acyltransferase TbDAT is dispensable for normal growth but important for synthesis of ether glycerophospholipids. PLoS ONE. 2017;12:e0181432 pubmed publisher
Pena F, Ball B, Squires E. A New Method for Evaluating Stallion Sperm Viability and Mitochondrial Membrane Potential in Fixed Semen Samples. Cytometry B Clin Cytom. 2018;94:302-311 pubmed publisher
Iraolagoitia X, Spallanzani R, Torres N, Araya R, Ziblat A, Domaica C, et al. NK Cells Restrain Spontaneous Antitumor CD8+ T Cell Priming through PD-1/PD-L1 Interactions with Dendritic Cells. J Immunol. 2016;197:953-61 pubmed publisher
Akabane S, Matsuzaki K, Yamashita S, Arai K, Okatsu K, Kanki T, et al. Constitutive Activation of PINK1 Protein Leads to Proteasome-mediated and Non-apoptotic Cell Death Independently of Mitochondrial Autophagy. J Biol Chem. 2016;291:16162-74 pubmed publisher
Kuo H, Lee D, Chen C, Liu M, Chou C, Lee H, et al. ARD1 stabilization of TSC2 suppresses tumorigenesis through the mTOR signaling pathway. Sci Signal. 2010;3:ra9 pubmed publisher

product information

Apps. Abbrev. :

Flow Cytometry, Intracellular Staining for Flow Cytometry, ICC

Cat # :

423112

Item :

Zombie Green™ Fixable Viability Kit

Other Names :

Fixable Dye, Fixable Viability Dye

Size :

500 tests

Price (USD) :

314 USD

Conjugate/Tag/Label :

NONAB_CHMPRB

Application Notes :

Standard Cell Staining Protocol: Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial. For reconstitution, pre-warm the kit to room temperature; add 100 ul of DMSO to one vial of Zombie Green dye and mix until fully dissolved Wash cells with PBS buffer (no Tris buffer and protein free). Dilute Zombie Green dye at 1:100-1000 in PBS. Resuspend 1-10 x 10 6 cells in diluted 100 ul Zombie Green solution. To minimize background staining of live cells, titrate the amount of dye and/or number of cells per 100 ul for optimal performance. Different cell types can have a wide degree of variability in staining based on cell size and degree of cell death. Note: Don t use Tris buffer as a diluent and be sure that the PBS does not contain any other protein like BSA or FBS. Note: The amount of dye used can also influence the ability to detect apoptotic as well as live and dead cells. Incubate the cells at room temperature, in the dark, for 15-30 minutes. Wash one time with 2 ml BioLegend s Cell Staining Buffer (Cat. No. 420201) or equivalent buffer containing serum or BSA. Continue performing antibody staining procedure as desired. Cells can be fixed with paraformaldehyde or methanol prior to permeabilization or can be analyzed without fixation. . . No-wash Sequential Staining Protocol: . Wash cells with PBS buffer (no Tris buffer and protein free). For reconstitution, pre-warm the kit to room temperature; add 100 ul of DMSO to one vial of Zombie Green dye and mix until fully dissolved Determine the total ul volume of antibody cocktail previously titrated and optimized for the assay that will be added to each vial/well of cells based on a final volume of 100 ul. Subtract that antibody volume from the 100 ul total staining volume intended for the assay. In the remaining volume, dilute Zombie Green dye at 1:100-1000 in PBS as determined by prior optimization at that volume. For example, if you are adding 20 ul of antibody cocktail for a 100 ul total staining volume, use 80 ul of Zombie Green solution. Resuspend 1-10 x 10 6 cells in the appropriate volume of Zombie Green solution. Different cell types can have a wide degree of variability in staining based on cell size and degree of cell death. Note: Don t use Tris buffer as a diluent and be sure that the PBS does not contain any other protein like BSA or FBS. Note: The amount of dye used can also influence the ability to detect apoptotic as well as live and dead cells. Incubate for 10-15 minutes at RT, protected from light. Without washing the cells, add the cell surface antibody cocktail and incubate for another 15-20 minutes. Add 1-2 mL Cell Staining Buffer (Cat. No. 420201) or equivalent buffer containing BSA or serum. Centrifuge to pellet. Continue with normal fixation and permeabilization procedure. If planning to skip fixation and analyze cells live, complete an additional wash step to minimize any unnecessary background of the live cells. . Notes: If the cell type in use cannot tolerate a protein-free environment, then titrate the Zombie Green dye in the presence of the same amount of BSA/serum as will be present in the antibody staining procedure. A higher amount of Zombie Green may be required since the BSA/serum will react with and bind up some proportion of the Zombie Green . Zombie Green dye is excited by the blue laser (488 nm) and has a fluorescence emission maximum of 515 nm. If using in a multi-color panel design, filter optimization may be required depending on other fluorophores used. Zombie Green dye has similar emission to FITC.