Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads

422606

2.5 mL

1538 USD

Cell Culture, Activ

422606

Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads

2.5 mL

1538 USD

Human

CV_BEADS

This product is designed for the in vitro or ex vivo expansion of human T cells.; Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads are a ready to use solution. There is no need to dilute the product prior to use in cell culture.; Suggested culture protocol:;Prepare T Cell Complete Media (TCCM) using sterile, filtered IMDM with 5% final concentration of serum and 200 IU/mL of human IL-2.;Prepare cells at a concentration of 1 x 10^6 per mL of TCCM.;Wash 25 µL of Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads to a 5 mL polypropylene tube with 1 mL TCCM.;Apply tube containing beads to a magnet (MojoSort magnet, cat#: 480019) for 2 minutes. Decant supernatant and remove tube from magnet.;Resuspend beads in 1 mL of cell suspension and plate in a 12 well cell culture plate.;Allow cells to expand at 37°C and 5% CO2. At D4 and D7, count cells and resuspend at 0.5 x 10^6 cells per mL.;Harvest cells at D10 or D11 for further analysis.;This product was optimized on isolated CD3+ T cells and PBMCs. Alternate sources of T cells may require protocol optimization.;