Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Intracellular Glutathione Assay Kit

421936

1 kit

425 USD

421936

Intracellular Glutathione Assay Kit

GSH assay kit, glutathione sensor, GSH probe, GSH Live Cell Assay, Glutathione Kit

1 kit

425 USD

Human

NONAB_CHMPRB

Detection/Imaging Guidelines:;- Fluorescence microscope filter set: GFP/FITC filter set;- Flow cytometry channels:;Excitation: 488 nm laser;Emission: 530/30 nm filter;Instrument specification(s): FITC channel;Components and storageThis kit is stored at -20°C. For convenience, the buffer and DMSO can be stored at 4°C.- 1 vial of Glutathione Probe Green (50 μL) (store at -20°C and minimize light exposure)- 1 bottle of Glutathione Assay Buffer (100 mL)- 1 vial of DMSO (500 μL)Required Materials Not Included:- For live cell imaging: tissue culture vessel with coverslip bottomAssay Protocol:This kit provides enough reagents for 100 tests (for up to 1x10^8 cells). A single test is defined as 1 mL of Glutathione Probe Green working solution for up to 1x10^6 cells.Preparation of 200X Glutathione Probe Green: Bring DMSO and Glutathione Probe Green provided in the kit to room temperature. Add 500 µL of DMSO into the vial containing 50 µL of Glutathione Probe Green Stock Solution and mix well to make 200X Glutathione Probe Green. If not using immediately, aliquot the 200X Glutathione Probe Green and store at -20°C protected from light. Avoid freeze thaw cycles. Experimental Protocol:;1. ;Bring all components of the kit to room temperature before starting. Treat your cell samples under desired experimental conditions in complete media. 2. ;Prepare the Glutathione Probe Green Working Solution by adding 5 µL of the 200X Glutathione Probe Green to 1 mL media for up to 1x10^6 cells. For larger cell amounts, scale up accordingly. 3. ;Gently remove media from your cells and add the Glutathione Probe Green Working Solution and incubate the samples at 37°C for 15-30 minutes. 4. ;For live cell imaging: Gently wash the cells 3 times with Glutathione Assay Buffer or buffer of choice and add the desired volume of Glutathione Assay Buffer or your buffer of choice for imaging. Observe cells with an inverted fluorescence microscope using the GFP/FITC filter set.For flow cytometry: Spin down cells and resuspend in the desired volume of Glutathione Assay Buffer or your buffer of choice. Monitor the fluorescence intensity on a flow cytometer using the FITC (530/30 nm) channel.;