Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Endoplasmic Reticulum Probe Green

421926

ea

400 USD

421926

Endoplasmic Reticulum Probe Green

ea

400 USD

Human

NONAB_CHMPRB

This probe employs a cell-permeant fluorescent dye that is selective for the endoplasmic reticulum in most cell types. Components:1 vial of lyophilized Endoplasmic Reticulum Probe Green1 vial of DMSOStorage:Upon receipt of the kit, store all components at -20°C and protected from light.Once the DMSO is added to the probe and reconstituted to make the 500X stock solution, store aliquots at -20°C and protected from light.Avoid freeze thaw cycles.Required Materials Not Included:Stain Buffer: Phenol-free cell culture media (without serum), HBSS or PBSImaging Guidelines:Ex/Em = 503/511 nm Fluorescence microscope filter set: FITCFlow Guidelines:Analysis in FITC or similar channelPreparation of 500X Endoplasmic Reticulum Stock Solution:;Thaw the probe and DMSO at room temperature before starting the experiment.;To make the 500X Endoplasmic Reticulum Stock Solution, add 20 μL of DMSO to the vial of Endoplasmic Reticulum Probe and mix well.;Store unused 500X Endoplasmic Reticulum Stock Solution at -20°C in single use aliquots to avoid freeze thaw cycles. Unused stock solution is stable for at least 2 weeks at ≤ -20°C. Live-Cell Imaging Protocol:;Prepare the 1X Endoplasmic Reticulum Staining Solution by diluting the 500X Endoplasmic Reticulum Stock Solution 1:500 in phenol-free media, HBSS or PBS and mix well.Note: Desired final volume to be determined by user. We recommend 100 μL per well in a 96-well plate.;Remove media from live cells and add the desired volume of 1X Endoplasmic Reticulum Staining Solution.;Optional: Include live-cell nuclear counterstain such as DRAQ5™ (Cat. No. 424101).;Incubate cells at 37°C for 15 - 30 minutes.;Remove the 1X Endoplasmic Reticulum Staining Solution.;Gently wash cells twice with phenol-free media, HBSS or PBS.;Proceed immediately to live-cell imaging.;Flow Cytometry Protocol;Prepare cell suspension with 0.25 - 2.5 x10^6 cells in 499 μL of phenol-free media, HBSS or PBS.;Add 1 μL of 500X Endoplasmic Reticulum Stock Solution to the cell suspension (1:500 dilution).Note: Staining conditions and probe concentration may have to be modified according to cell type and assay used.;Incubate cells at room temperature for 15 - 30 minutes.;Wash cells twice with PBS or equivalent buffer and then add desired volume of the same buffer.;Proceed to analysis using a Flow Cytometer.;