Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Lysosome Probe NIR

421916

1 vial

250 USD

421916

Lysosome Probe NIR

1 vial

250 USD

Human, Mouse

NONAB_CHMPRB

Component:20 μL of 500X Lysosome Probe NIR (-20°C, protected from light, avoid freeze/thaw cycles)Required Materials Not Included:Buffer: PBS, phenol-free media, or HBSS Detection/Imaging Guidelines:Ex/Em = 636/650 nm Fluorescence microscope filter set: Cyanine5Flow cytometry channel: APC channel Assay Protocol:;Thaw the 500X Lysosome Probe and allow it to reach room temperature.;Prepare lysosome staining solution by diluting the Lysosome Probe 1:500 in your buffer of choice.Note: Probe concentration may need to be adjusted according to cell type and assay conditions.;Optional: Include live-cell nuclear counterstain such as CytoPhase™ Violet (cat# 425701) or Hoechst 33342.;Add an equal volume of Lysosome Staining solution to cell culture media.;Incubate the cells at 37°C for 30 minutes.;Wash cells by twice with PBS or HBSS. If cells are in suspension, spin down and resuspend in buffer of choice.;Add desired volume of PBS or HBSS to cells for observation.;Proceed immediately to analysis.;