Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Golgi Detection Probe Red

421908

ea

500 USD

421908

Golgi Detection Probe Red

TMR Ceramide Golgi Probe

ea

500 USD

Human

NONAB_CHMPRB

The Golgi Detection Probe Red is a fluorescently labeled ceramide, which selectively binds to the Golgi apparatus and provides a simple method to examine Golgi morphology in live cells. Using the recommended volume of 100 μL per well in a 96-well plate, this product supplies enough probe for 100 tests. Component:Lyophilized Golgi Detection Probe, 1 vialDMSO, 1 vialRequired Materials Not Included:Phenol-free media or HBSSImaging Guidelines:Ex/Em = 544/570 nm Fluorescence microscope filter set: Cyanine3/TRITC filterFlow Guidelines:Analysis in Cyanine3 or similar channelAssay Protocol:1. Prepare the 100X Golgi Detection Probe Stock Solution by adding 100 µL of DMSO to the lyophilized probe. Gently mix until dissolved.Note: Store the unused Golgi Detection Probe Stock Solution at -20°C in single use aliquots to avoid freeze thaw cycles.2. Allow the 100X Golgi Detection Probe Stock Solution to reach room temperature.3. Prepare Golgi Detection Probe Working Solution by diluting 1:100 in Phenol-free media or HBSSNote: Staining conditions and probe concentration may have to be modified according to cell type and assay used.4. Remove growth media from cells and add the Golgi Detection Probe Working Solution.Note: We recommend 100 µL per well for a 96-well plate.5. Incubate cells at 37°C for 20-30 minutes.6. Wash cells twice with phenol free media or HBSS and then add desired volume of phenol-free media or HBSS.7. Proceed immediately to live-cell imaging.;