Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Cell-Vive™ MSC Xeno-Free Growth Media, GMP

420519

250 mL

275 USD

420519

Cell-Vive™ MSC Xeno-Free Growth Media, GMP

Mesenchymal Stromal Cells, Medicinal Signaling Cells, Multipotent Stromal Cells

250 mL

275 USD

CV

This product is a complete media formulation that is ready to use for culturing bone marrow-, adipose tissue- or umbilical cord tissue-derived mesenchymal stem cells. It can be used without any cell attachment. However, pending on the tissue sourced MSCs, recombinant human fibronectin (Cat#775314) can be used to further optimize the expansion.; The presence of trace precipitation in the product after thaw is normal and relates to the high enrichment of the product and should not impact its performance. The precipitates should go into solution after warming up the media in 37°C water bath or equivalent, or if desired, precipitates can be removed through 0.22 µm filtration of the media without impacting its performance.; Cell Culture Platform:;Cell-Vive™ MSC Xeno-Free Growth Media, GMP can be used with tissue culture plates, and flasks. Further protocol optimization is recommended with the specific bioreactor being used.; MSC culture from cryopreserved human MSCs:;Thaw media overnight or longer until fully thawed at 2-8°C.;Pre-warm planned volume of completed thawed with Cell-Vive™ MSC Xeno-Free Growth Media to 37°C.;Rapidly thaw frozen vial of cells in a 37°C water bath. After the vial is 80-90% thawed, pipet the entire contents of the cryovial into a 15 mL conical tube. Carefully add 5 to 10 mL of pre-warmed Cell-Vive™ MSC Xeno-Free Growth Media at an approximate rate of three to five drops per ten seconds and gently swirl after every addition.;After brief mixing, centrifuge the 15 mL conical tubes at 1500 rpm for 5 min. Decant (or aspirate) the supernatant. Add 2 mL or desired volume of pre-warmed Cell-Vive™ MSC Xeno-Free Growth Media, gently mix the cells with a sterile 1 mL serological pipette. Count the cells and viability with hemocytometer, or equivalent.;Resuspend cells and seed MSCs at 6,000 cells/cm^2 density. Incubate the cells at 37°C, 5% CO2. Aspirate off media and feed the cells with pre-warmed Cell-Vive™ MSC Xeno-Free Growth Media 24 hours after seeding.;Every two days, remove and discard spent media, and feed the cells with pre-warmed Cell-Vive™ MSC Xeno-Free Growth Media;Subculture cells once they reach 80% confluence. Do not allow the cultures to become 100% or over confluent. Subculturing under suboptimal conditions may affect product performance.;