Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP

420518

50 mL

195 USD

420518

Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP

Cryo Solution, cropreservation media

50 mL

195 USD

CV

For cryopreservation, a range between 0.5-5 million cells per 1 mL of Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP is recommended. The liquid product is clear in appearance.; Cryopreservation protocol:;Prepare a cell suspension following an appropriate protocol.;Centrifuge cells at 300 x g for 5 minutes to obtain a pellet. Carefully aspirate supernatant;Resuspend pelleted cells with Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP and dispense the cell suspension into a cryovial.;Immediately transfer the cryovial into a cell freezing container and store it at -80°C.;After 24-36 hours, transfer cryovials into a liquid nitrogen tank for long-term storage.;Thawing protocol;Thaw cryovials containing cells by gently swirling them in a 37°C water bath. Thaw until only a small ice fragment is present.;In a biosafety hood, add the thawed cryopreservation solution into 8 mL of preferred cell culture media in a 15 mL conical tube. Wash the vial with 1 mL of cell culture media and add to the conical tube containing cells. Gently mix.;Centrifuge and remove the supernatant.;Resuspend the cells at the desired density with cell culture media.;