Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP

420516

100 mL

220 USD

420516

Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP

Cryo Solution, DMSO-free, Cryopreservation media

100 mL

220 USD

CV

For cryopreservation, a range between 0.5-5 million cells per 1 mL of Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP is recommended. The appearance of the product in liquid form is clear with a slight pink color.; MSCs Cryopreservation protocol:;Prepare a MSC suspension following an appropriate protocol.;Centrifuge cells at 300 x g for 5 minutes to obtain a pellet. Carefully aspirate supernatant.;Resuspend pelleted cells with Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP and dispense the cell suspension into a cryovial.;Immediately transfer the cryovial into a cell freezing container and store it at -70°C;After 24-36 hours, transfer cryovials into a liquid nitrogen tank for long-term storage.; Thawing MSCs protocol:;Thaw cryovials containing cells by putting them in a 37°C water bath with very gently swirling. Thaw until only a small ice fragment is present.;In a biosafety hood, add 1 mL of preferred cell culture media into the vial and then transfer content into a 15 mL conical tube containing 8 mL of cell culture media at room temperature. Gently mix.;Centrifuge and remove the supernatant.;Resuspend the cells at the desired density with cell culture media.;