Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Cell-Vive™ CD Low DMSO Cryopreservation, GMP

420504

50 mL

165 USD

420504

Cell-Vive™ CD Low DMSO Cryopreservation, GMP

Chemically-defined Low DMSO Cryopreservation Solution

50 mL

165 USD

CV

For cryopreservation, a range between 5-50 million cells per 1 mL of Cell-Vive™ CD Low DMSO Cryopreservation, GMP is recommended. The appearance of the product in liquid form is clear with a slight yellow color. Cryopreservation protocol;Prepare an immune cells suspension following an appropriate protocol.;Centrifuge cells at 300 x g for 5 minutes to obtain a pellet. Carefully decant or aspirate supernatant;Resuspend pelleted cells with Cell-Vive™ CD Low DMSO Cryopreservation, GMP and dispense the cell suspension into a cryovial.;Immediately transfer the cryovial into a cell freezing container and store it at -70°C;After 24-36 hours, transfer cryovials into a liquid nitrogen tank for long-term storage.;Thawing cells protocol;Thaw cryovials containing cells by putting them in a 37°C water bath with very gently swirling. Thaw until only a small ice fragment is present.;In a biosafety hood, add 1 mL of preferred cell culture media such as IMDM into the vial and then transfer content into a 15 mL conical tube containing 8mL of cell culture media at room temperature. Gently mix;Centrifuge cells at 300 x g for 5 minutes. Carefully decant or aspirate supernatant;Resuspend the cells at the desired density with cell culture media.;