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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-mouse IgD
catalog :
405707
quantity :
25 μg
price :
112 USD
clonality :
monoclonal
host :
rat
conjugate :
AF647
clone name :
11-26c.2a
reactivity :
mouse
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 85
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...; fig s17a, s19a
Satofuka H, Abe S, Moriwaki T, Okada A, Kazuki K, Tanaka H, et al. Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci. Nat Commun. 2022;13:1841 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 4b, 5a
Aslam M, Alemdehy M, Kwesi Maliepaard E, Muhaimin F, Caganova M, Pardieck I, et al. Histone methyltransferase DOT1L controls state-specific identity during B cell differentiation. EMBO Rep. 2021;22:e51184 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 2d
Stebegg M, Bignon A, Hill D, Silva Cayetano A, Krueger C, Vanderleyden I, et al. Rejuvenating conventional dendritic cells and T follicular helper cell formation after vaccination. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Graf R, Seagal J, Otipoby K, Lam K, Ayoub S, Zhang B, et al. BCR-dependent lineage plasticity in mature B cells. Science. 2019;363:748-753 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3b
Tang C, Chang S, Paton A, Paton J, Gabrilovich D, Ploegh H, et al. Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization. J Cell Biol. 2018;217:1739-1755 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Dahlgren M, Gustafsson Hedberg T, Livingston M, Cucak H, Alsén S, Yrlid U, et al. T follicular helper, but not Th1, cell differentiation in the absence of conventional dendritic cells. J Immunol. 2015;194:5187-99 pubmed publisher
Yazicioglu Y, Marin E, Sandhu C, Galiani S, Raza I, Ali M, et al. Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis. Nat Immunol. 2023;24:991-1006 pubmed publisher
Hodgson R, Xu X, Anzilotti C, Deobagkar Lele M, Crockford T, Kepple J, et al. NDRG1 is induced by antigen-receptor signaling but dispensable for B and T cell self-tolerance. Commun Biol. 2022;5:1216 pubmed publisher
Lee J, Fra Bido S, Burton A, Innocentin S, Hill D, Linterman M. B cell-intrinsic changes with age do not impact antibody-secreting cell formation but delay B cell participation in the germinal centre reaction. Aging Cell. 2022;21:e13692 pubmed publisher
Fleig S, Kapanadze T, Bernier Latmani J, Lill J, Wyss T, Gamrekelashvili J, et al. Loss of vascular endothelial notch signaling promotes spontaneous formation of tertiary lymphoid structures. Nat Commun. 2022;13:2022 pubmed publisher
He Y, Gallman A, Xie C, Shen Q, Ma J, Wolfreys F, et al. P2RY8 variants in lupus patients uncover a role for the receptor in immunological tolerance. J Exp Med. 2022;219: pubmed publisher
Stephens W, Kubinak J, Ghazaryan A, Bauer K, Bell R, Buhrke K, et al. Epithelial-myeloid exchange of MHC class II constrains immunity and microbiota composition. Cell Rep. 2021;37:109916 pubmed publisher
Duan L, Liu D, Chen H, Mintz M, Chou M, Kotov D, et al. Follicular dendritic cells restrict interleukin-4 availability in germinal centers and foster memory B cell generation. Immunity. 2021;54:2256-2272.e6 pubmed publisher
Monz xf3 n Casanova E, Bates K, Smith C, Turner M. Essential requirement for polypyrimidine tract binding proteins 1 and 3 in the maturation and maintenance of mature B cells in mice. Eur J Immunol. 2021;51:2266-2273 pubmed publisher
Fra Bido S, Walker S, Innocentin S, Linterman M. Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice. STAR Protoc. 2021;2:100499 pubmed publisher
Gallman A, Wolfreys F, Nguyen D, Sandy M, Xu Y, An J, et al. Abcc1 and Ggt5 support lymphocyte guidance through export and catabolism of S-geranylgeranyl-l-glutathione. Sci Immunol. 2021;6: pubmed publisher
Silva Cayetano A, Foster W, Innocentin S, Belij Rammerstorfer S, Spencer A, Burton O, et al. A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice. Med (N Y). 2021;2:243-262.e8 pubmed publisher
Lederer K, Castaño D, Gomez Atria D, Oguin T, Wang S, Manzoni T, et al. SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation. Immunity. 2020;53:1281-1295.e5 pubmed publisher
Zhong M, Lu Y, Qian J, Zhu Y, Dong L, Zahn A, et al. SLAM family receptors control pro-survival effectors in germinal center B cells to promote humoral immunity. J Exp Med. 2021;218: pubmed publisher
Crouse B, Robinson C, Huseby Kelcher A, Laudenbach M, Abrahante J, Pravetoni M. Mechanisms of interleukin 4 mediated increase in efficacy of vaccines against opioid use disorders. NPJ Vaccines. 2020;5:99 pubmed publisher
Soon M, Lee H, Engel J, Straube J, Thomas B, Pernold C, et al. Transcriptome dynamics of CD4+ T cells during malaria maps gradual transit from effector to memory. Nat Immunol. 2020;21:1597-1610 pubmed publisher
Siamishi I, Iwanami N, Clapes T, Trompouki E, O Meara C, Boehm T. Lymphocyte-Specific Function of the DNA Polymerase Epsilon Subunit Pole3 Revealed by Neomorphic Alleles. Cell Rep. 2020;31:107756 pubmed publisher
Biram A, Winter E, Denton A, Zaretsky I, Dassa B, Bemark M, et al. B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches. Cell Rep. 2020;30:1910-1922.e5 pubmed publisher
Vanderleyden I, Fra Bido S, Innocentin S, Stebegg M, Okkenhaug H, Evans Bailey N, et al. Follicular Regulatory T Cells Can Access the Germinal Center Independently of CXCR5. Cell Rep. 2020;30:611-619.e4 pubmed publisher
Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
Xu H, Ding J, Porter C, Wallrapp A, Tabaka M, Ma S, et al. Transcriptional Atlas of Intestinal Immune Cells Reveals that Neuropeptide α-CGRP Modulates Group 2 Innate Lymphoid Cell Responses. Immunity. 2019;51:696-708.e9 pubmed publisher
Vacchio M, Ciucci T, Gao Y, Watanabe M, Balmaceno Criss M, McGinty M, et al. A Thpok-Directed Transcriptional Circuitry Promotes Bcl6 and Maf Expression to Orchestrate T Follicular Helper Differentiation. Immunity. 2019;51:465-478.e6 pubmed publisher
Zhu W, Naler L, Maul R, Sallin M, Sen J. Immune system development and age-dependent maintenance in Klotho-hypomorphic mice. Aging (Albany NY). 2019;11:5246-5257 pubmed publisher
Clancy Thompson E, Chen G, LaMarche N, Ali L, Jeong H, Crowley S, et al. Transnuclear mice reveal Peyer's patch iNKT cells that regulate B-cell class switching to IgG1. EMBO J. 2019;38:e101260 pubmed publisher
Mintz M, Felce J, Chou M, Mayya V, Xu Y, Shui J, et al. The HVEM-BTLA Axis Restrains T Cell Help to Germinal Center B Cells and Functions as a Cell-Extrinsic Suppressor in Lymphomagenesis. Immunity. 2019;: pubmed publisher
Nakai A, Fujimoto J, Miyata H, Stumm R, Narazaki M, Schulz S, et al. The COMMD3/8 complex determines GRK6 specificity for chemoattractant receptors. J Exp Med. 2019;: pubmed publisher
Schrock D, Leddon S, Hughson A, Miller J, Lacy Hulbert A, Fowell D. Pivotal role for αV integrins in sustained Tfh support of the germinal center response for long-lived plasma cell generation. Proc Natl Acad Sci U S A. 2019;: pubmed publisher
Faliti C, Gualtierotti R, Rottoli E, Gerosa M, Perruzza L, Romagnani A, et al. P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus. J Exp Med. 2019;216:317-336 pubmed publisher
Tan H, Jegaskanda S, Juno J, Esterbauer R, Wong J, Kelly H, et al. Subdominance and poor intrinsic immunogenicity limit humoral immunity targeting influenza HA stem. J Clin Invest. 2019;129:850-862 pubmed publisher
Kiyohara H, Sujino T, Teratani T, Miyamoto K, Arai M, Nomura E, et al. Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells. Cell Mol Gastroenterol Hepatol. 2019;7:135-156 pubmed publisher
Cheng Q, Liu J, Pei Y, Zhang Y, Zhou D, Pan W, et al. Neddylation contributes to CD4+ T cell-mediated protective immunity against blood-stage Plasmodium infection. PLoS Pathog. 2018;14:e1007440 pubmed publisher
Irons E, Lau J. Systemic ST6Gal-1 Is a Pro-survival Factor for Murine Transitional B Cells. Front Immunol. 2018;9:2150 pubmed publisher
Fistonich C, Zehentmeier S, Bednarski J, Miao R, Schjerven H, Sleckman B, et al. Cell circuits between B cell progenitors and IL-7+ mesenchymal progenitor cells control B cell development. J Exp Med. 2018;215:2586-2599 pubmed publisher
Preite S, Cannons J, Radtke A, Vujkovic Cvijin I, Gomez Rodriguez J, Volpi S, et al. Hyperactivated PI3Kδ promotes self and commensal reactivity at the expense of optimal humoral immunity. Nat Immunol. 2018;19:986-1000 pubmed publisher
Cao A, Alluqmani N, Buhari F, Wasim L, Smith L, Quaile A, et al. Galectin-9 binds IgM-BCR to regulate B cell signaling. Nat Commun. 2018;9:3288 pubmed publisher
Lin Y, Pecetta S, Steichen J, Kratochvil S, Melzi E, Arnold J, et al. One-step CRISPR/Cas9 method for the rapid generation of human antibody heavy chain knock-in mice. EMBO J. 2018;37: pubmed publisher
Guo H, Barberi T, Suresh R, Friedman A. Progression from the Common Lymphoid Progenitor to B/Myeloid PreproB and ProB Precursors during B Lymphopoiesis Requires C/EBPα. J Immunol. 2018;201:1692-1704 pubmed publisher
Avery D, Kane A, Nguyen T, Lau A, Nguyen A, Lenthall H, et al. Germline-activating mutations in PIK3CD compromise B cell development and function. J Exp Med. 2018;215:2073-2095 pubmed publisher
Schabla N, Perry G, Palmer V, Swanson P. VprBP (DCAF1) Regulates RAG1 Expression Independently of Dicer by Mediating RAG1 Degradation. J Immunol. 2018;201:930-939 pubmed publisher
Li R, Liu J, Wu S, Zai X, Li Y, Yang Q, et al. Toll-like receptor 4 signalling regulates antibody response to adenoviral vector-based vaccines by imprinting germinal centre quality. Immunology. 2018;155:251-262 pubmed publisher
Seydoux E, Liang H, Dubois Cauwelaert N, Archer M, Rintala N, Kramer R, et al. Effective Combination Adjuvants Engage Both TLR and Inflammasome Pathways To Promote Potent Adaptive Immune Responses. J Immunol. 2018;201:98-112 pubmed publisher
Barwick B, Scharer C, Martinez R, Price M, Wein A, Haines R, et al. B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation. Nat Commun. 2018;9:1900 pubmed publisher
Rodda L, Lu E, Bennett M, Sokol C, Wang X, Luther S, et al. Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity. Immunity. 2018;48:1014-1028.e6 pubmed publisher
Valentine K, Davini D, Lawrence T, Mullins G, Manansala M, Al Kuhlani M, et al. CD8 Follicular T Cells Promote B Cell Antibody Class Switch in Autoimmune Disease. J Immunol. 2018;201:31-40 pubmed publisher
Pardi N, Hogan M, Naradikian M, Parkhouse K, Cain D, Jones L, et al. Nucleoside-modified mRNA vaccines induce potent T follicular helper and germinal center B cell responses. J Exp Med. 2018;215:1571-1588 pubmed publisher
Hwang I, Boularan C, Harrison K, Kehrl J. Gαi Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression. Front Immunol. 2018;9:687 pubmed publisher
Chen Y, Chaudhary N, Yang N, Granato A, Turner J, Howard S, et al. Microbial symbionts regulate the primary Ig repertoire. J Exp Med. 2018;215:1397-1415 pubmed publisher
Bröker K, Figge J, Magnusen A, Manz R, Köhl J, Karsten C. A Novel Role for C5a in B-1 Cell Homeostasis. Front Immunol. 2018;9:258 pubmed publisher
Yamazaki T, Nagashima M, Ninomiya D, Ainai A, Fujimoto A, Ichimonji I, et al. Neutralizing Antibodies Induced by Gene-Based Hydrodynamic Injection Have a Therapeutic Effect in Lethal Influenza Infection. Front Immunol. 2018;9:47 pubmed publisher
Duncan C, Kondilis Mangum H, Grimm S, Bushel P, Chrysovergis K, Roberts J, et al. Base-Resolution Analysis of DNA Methylation Patterns Downstream of Dnmt3a in Mouse Naïve B Cells. G3 (Bethesda). 2018;8:805-813 pubmed publisher
Hsu C, Meeker S, Escobar S, Brabb T, Paik J, Park H, et al. Murine norovirus inhibits B cell development in the bone marrow of STAT1-deficient mice. Virology. 2018;515:123-133 pubmed publisher
Suan D, Kräutler N, Mååg J, Butt D, Bourne K, Hermes J, et al. CCR6 Defines Memory B Cell Precursors in Mouse and Human Germinal Centers, Revealing Light-Zone Location and Predominant Low Antigen Affinity. Immunity. 2017;47:1142-1153.e4 pubmed publisher
Sutavani R, Phair I, Barker R, McFarlane A, Shpiro N, Lang S, et al. Differential control of Toll-like receptor 4-induced interleukin-10 induction in macrophages and B cells reveals a role for p90 ribosomal S6 kinases. J Biol Chem. 2018;293:2302-2317 pubmed publisher
Adori M, Pedersen G, Adori C, Erikson E, Khoenkhoen S, Stark J, et al. Altered Marginal Zone B Cell Selection in the Absence of I?BNS. J Immunol. 2018;200:775-787 pubmed publisher
Shi B, Geng J, Wang Y, Wei H, Walters B, Li W, et al. Foxp1 Negatively Regulates T Follicular Helper Cell Differentiation and Germinal Center Responses by Controlling Cell Migration and CTLA-4. J Immunol. 2018;200:586-594 pubmed publisher
Wang Q, Racine J, Ratiu J, Wang S, Ettinger R, Wasserfall C, et al. Transient BAFF Blockade Inhibits Type 1 Diabetes Development in Nonobese Diabetic Mice by Enriching Immunoregulatory B Lymphocytes Sensitive to Deletion by Anti-CD20 Cotherapy. J Immunol. 2017;199:3757-3770 pubmed publisher
Gibb D, Liu J, Santhanakrishnan M, Natarajan P, Madrid D, Patel S, et al. B cells require Type 1 interferon to produce alloantibodies to transfused KEL-expressing red blood cells in mice. Transfusion. 2017;57:2595-2608 pubmed publisher
Soto R, Petersen C, Novis C, Kubinak J, Bell R, Stephens W, et al. Microbiota promotes systemic T-cell survival through suppression of an apoptotic factor. Proc Natl Acad Sci U S A. 2017;114:5497-5502 pubmed publisher
Ratiu J, Racine J, Hasham M, Wang Q, Branca J, Chapman H, et al. Genetic and Small Molecule Disruption of the AID/RAD51 Axis Similarly Protects Nonobese Diabetic Mice from Type 1 Diabetes through Expansion of Regulatory B Lymphocytes. J Immunol. 2017;198:4255-4267 pubmed publisher
Sindhava V, Oropallo M, Moody K, Naradikian M, Higdon L, Zhou L, et al. A TLR9-dependent checkpoint governs B cell responses to DNA-containing antigens. J Clin Invest. 2017;127:1651-1663 pubmed publisher
Jandl C, Liu S, Canete P, Warren J, Hughes W, Vogelzang A, et al. IL-21 restricts T follicular regulatory T cell proliferation through Bcl-6 mediated inhibition of responsiveness to IL-2. Nat Commun. 2017;8:14647 pubmed publisher
Dotsey E, Ushach I, Pone E, Nakajima R, Jasinskas A, Argueta D, et al. Transient Cannabinoid Receptor 2 Blockade during Immunization Heightens Intensity and Breadth of Antigen-specific Antibody Responses in Young and Aged mice. Sci Rep. 2017;7:42584 pubmed publisher
Robinson M, Prout M, Mearns H, Kyle R, Camberis M, Forbes Blom E, et al. IL-4 Haploinsufficiency Specifically Impairs IgE Responses against Allergens in Mice. J Immunol. 2017;198:1815-1822 pubmed publisher
Suzuki K, Hayano Y, Nakai A, Furuta F, Noda M. Adrenergic control of the adaptive immune response by diurnal lymphocyte recirculation through lymph nodes. J Exp Med. 2016;213:2567-2574 pubmed
Volkmann C, Brings N, Becker M, Hobeika E, Yang J, Reth M. Molecular requirements of the B-cell antigen receptor for sensing monovalent antigens. EMBO J. 2016;35:2371-2381 pubmed
Parsa R, Lund H, Georgoudaki A, Zhang X, Ortlieb Guerreiro Cacais A, Grommisch D, et al. BAFF-secreting neutrophils drive plasma cell responses during emergency granulopoiesis. J Exp Med. 2016;213:1537-53 pubmed publisher
Leeth C, Racine J, Chapman H, Arpa B, Carrillo J, Carrascal J, et al. B-lymphocytes expressing an Ig specificity recognizing the pancreatic ß-cell autoantigen peripherin are potent contributors to type 1 diabetes development in NOD mice. Diabetes. 2016;65:1977-1987 pubmed publisher
Maity P, Blount A, Jumaa H, Ronneberger O, Lillemeier B, Reth M. B cell antigen receptors of the IgM and IgD classes are clustered in different protein islands that are altered during B cell activation. Sci Signal. 2015;8:ra93 pubmed publisher
Ogbe A, Miao T, Symonds A, Omodho B, Singh R, Bhullar P, et al. Early Growth Response Genes 2 and 3 Regulate the Expression of Bcl6 and Differentiation of T Follicular Helper Cells. J Biol Chem. 2015;290:20455-65 pubmed publisher
Rouaud P, Saintamand A, Saad F, Carrion C, Lecardeur S, Cogne M, et al. Elucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region. J Exp Med. 2014;211:975-85 pubmed publisher
Lindner J, Wong C, Möller A, Nielsen P. A C-terminal acidic domain regulates degradation of the transcriptional coactivator Bob1. Mol Cell Biol. 2013;33:4628-40 pubmed publisher
Park S, Wolfram P, Canty K, Harley B, Nombela Arrieta C, Pivarnik G, et al. Focal adhesion kinase regulates the localization and retention of pro-B cells in bone marrow microenvironments. J Immunol. 2013;190:1094-102 pubmed publisher
Kishi Y, Higuchi T, Phoon S, Sakamaki Y, Kamiya K, Riemekasten G, et al. Apoptotic marginal zone deletion of anti-Sm/ribonucleoprotein B cells. Proc Natl Acad Sci U S A. 2012;109:7811-6 pubmed publisher
Vu T, Gatto D, Turner V, Funnell A, Mak K, Norton L, et al. Impaired B cell development in the absence of Krüppel-like factor 3. J Immunol. 2011;187:5032-42 pubmed publisher
Gatto D, Wood K, Brink R. EBI2 operates independently of but in cooperation with CXCR5 and CCR7 to direct B cell migration and organization in follicles and the germinal center. J Immunol. 2011;187:4621-8 pubmed publisher
Lahoud M, Ahmet F, Kitsoulis S, Wan S, Vremec D, Lee C, et al. Targeting antigen to mouse dendritic cells via Clec9A induces potent CD4 T cell responses biased toward a follicular helper phenotype. J Immunol. 2011;187:842-50 pubmed publisher
Tomayko M, Steinel N, Anderson S, Shlomchik M. Cutting edge: Hierarchy of maturity of murine memory B cell subsets. J Immunol. 2010;185:7146-50 pubmed publisher
Good Jacobson K, Szumilas C, Chen L, Sharpe A, Tomayko M, Shlomchik M. PD-1 regulates germinal center B cell survival and the formation and affinity of long-lived plasma cells. Nat Immunol. 2010;11:535-42 pubmed publisher
Chan T, Gatto D, Wood K, Camidge T, Basten A, Brink R. Antigen affinity controls rapid T-dependent antibody production by driving the expansion rather than the differentiation or extrafollicular migration of early plasmablasts. J Immunol. 2009;183:3139-49 pubmed publisher
Ahuja A, Shupe J, Dunn R, Kashgarian M, Kehry M, Shlomchik M. Depletion of B cells in murine lupus: efficacy and resistance. J Immunol. 2007;179:3351-61 pubmed
product information
Antigen :
IgD
Apps. Abbrev. :
FC, 3D IHC
Cat # :
405707
Clone :
11-26c.2a
Item :
Alexa Fluor® 647 anti-mouse IgD
Isotype :
Rat IgG2a, κ
Other Names :
Immunoglobulin D
Size :
25 μg
Price (USD) :
112 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A647
Application Notes :
The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^2,3, and spatial biology (IBEX)^10,11.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA