This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
LEAF™ Purified Rat IgG2b, κ Isotype Ctrl
catalog :
400637
quantity :
1 mg
price :
175 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
RTK4530
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 32
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
Matsubara Y, Chiba T, Kashimada K, Morio T, Takada S, Mizutani S, et al. Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus. Sci Rep. 2014;4:5043 pubmed publisher
Yamashita M, Passegue E. TNF-α Coordinates Hematopoietic Stem Cell Survival and Myeloid Regeneration. Cell Stem Cell. 2019;: pubmed publisher
Mizuno R, Sugiura D, Shimizu K, Maruhashi T, Watada M, Okazaki I, et al. PD-1 Primarily Targets TCR Signal in the Inhibition of Functional T Cell Activation. Front Immunol. 2019;10:630 pubmed publisher
Yahata T, Mizoguchi M, Kimura A, Orimo T, Toujima S, Kuninaka Y, et al. Programmed cell death ligand 1 disruption by clustered regularly interspaced short palindromic repeats/Cas9-genome editing promotes antitumor immunity and suppresses ovarian cancer progression. Cancer Sci. 2019;110:1279-1292 pubmed publisher
Mastroianni J, Stickel N, Andrlova H, Hanke K, Melchinger W, Duquesne S, et al. miR-146a Controls Immune Response in the Melanoma Microenvironment. Cancer Res. 2019;79:183-195 pubmed publisher
Vonberg A, Acevedo Calado M, Cox A, Pietropaolo S, Gianani R, Lundy S, et al. CD19+IgM+ cells demonstrate enhanced therapeutic efficacy in type 1 diabetes mellitus. JCI Insight. 2018;3: pubmed publisher
Atretkhany K, Mufazalov I, Dunst J, Kuchmiy A, Gogoleva V, Andruszewski D, et al. Intrinsic TNFR2 signaling in T regulatory cells provides protection in CNS autoimmunity. Proc Natl Acad Sci U S A. 2018;115:13051-13056 pubmed publisher
He Y, Yuan X, Li Y, Zhong C, Liu Y, Qian H, et al. Loss of Gαq impairs regulatory B-cell function. Arthritis Res Ther. 2018;20:186 pubmed publisher
Hirano Y, Ode Y, Ochani M, Wang P, Aziz M. Targeting junctional adhesion molecule-C ameliorates sepsis-induced acute lung injury by decreasing CXCR4+ aged neutrophils. J Leukoc Biol. 2018;104:1159-1171 pubmed publisher
Vendetti F, Karukonda P, Clump D, Teo T, Lalonde R, Nugent K, et al. ATR kinase inhibitor AZD6738 potentiates CD8+ T cell-dependent antitumor activity following radiation. J Clin Invest. 2018;128:3926-3940 pubmed publisher
Ismail O, Sriranganathan S, Zhang X, Bonventre J, Zervos A, Gunaratnam L. Tctex-1, a novel interaction partner of Kidney Injury Molecule-1, is required for efferocytosis. J Cell Physiol. 2018;233:6877-6895 pubmed publisher
Lin D, Hiron T, O Callaghan C. Intragenic transcriptional interference regulates the human immune ligand MICA. EMBO J. 2018;37: pubmed publisher
Carrasco S, Hu S, Imai D, Kumar R, Sandusky G, Yang X, et al. Toll-like receptor 3 (TLR3) promotes the resolution of Chlamydia muridarum genital tract infection in congenic C57BL/6N mice. PLoS ONE. 2018;13:e0195165 pubmed publisher
Shevchenko M, Bogorodskiy A, Troyanova N, Servuli E, Bolkhovitina E, Büldt G, et al. Aspergillus fumigatus Infection-Induced Neutrophil Recruitment and Location in the Conducting Airway of Immunocompetent, Neutropenic, and Immunosuppressed Mice. J Immunol Res. 2018;2018:5379085 pubmed publisher
Mencarelli A, Vacca M, Khameneh H, Acerbi E, Tay A, Zolezzi F, et al. Calcineurin B in CD4+ T Cells Prevents Autoimmune Colitis by Negatively Regulating the JAK/STAT Pathway. Front Immunol. 2018;9:261 pubmed publisher
Li N, Yang H, Wang M, Lü S, Zhang Y, Long M. Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling. Mol Biol Cell. 2018;29:408-418 pubmed publisher
Huang G, Qi M, Li X, Tang F, Zhu L. Involvement of upregulation of fibronectin in the pro‑adhesive and pro‑survival effects of glucocorticoid on melanoma cells. Mol Med Rep. 2018;17:3380-3387 pubmed publisher
Kortlever R, Sodir N, Wilson C, Burkhart D, Pellegrinet L, Brown Swigart L, et al. Myc Cooperates with Ras by Programming Inflammation and Immune Suppression. Cell. 2017;171:1301-1315.e14 pubmed publisher
Dalmas E, Lehmann F, Dror E, Wueest S, Thienel C, Borsigova M, et al. Interleukin-33-Activated Islet-Resident Innate Lymphoid Cells Promote Insulin Secretion through Myeloid Cell Retinoic Acid Production. Immunity. 2017;47:928-942.e7 pubmed publisher
Mitroulis I, Chen L, Singh R, Kourtzelis I, Economopoulou M, Kajikawa T, et al. Secreted protein Del-1 regulates myelopoiesis in the hematopoietic stem cell niche. J Clin Invest. 2017;127:3624-3639 pubmed publisher
Ishidome T, Yoshida T, Hanayama R. Induction of Live Cell Phagocytosis by a Specific Combination of Inflammatory Stimuli. EBioMedicine. 2017;22:89-99 pubmed publisher
Soto Feliciano Y, Bartlebaugh J, Liu Y, Sanchez Rivera F, Bhutkar A, Weintraub A, et al. PHF6 regulates phenotypic plasticity through chromatin organization within lineage-specific genes. Genes Dev. 2017;31:973-989 pubmed publisher
De Giorgi M, Enjyoji K, Jiang G, Csizmadia E, Mitsuhashi S, Gumina R, et al. Complete deletion of Cd39 is atheroprotective in apolipoprotein E-deficient mice. J Lipid Res. 2017;58:1292-1305 pubmed publisher
Jiang J, Liu C, Liu M, Shen Y, Hu X, Wang Q, et al. OX40 signaling is involved in the autoactivation of CD4+CD28- T cells and contributes to the pathogenesis of autoimmune arthritis. Arthritis Res Ther. 2017;19:67 pubmed publisher
Mukai K, Karasuyama H, Kabashima K, Kubo M, Galli S. Differences in the Importance of Mast Cells, Basophils, IgE, and IgG versus That of CD4+ T Cells and ILC2 Cells in Primary and Secondary Immunity to Strongyloides venezuelensis. Infect Immun. 2017;85: pubmed publisher
Sung S, Ge Y, Dai C, Wang H, Fu S, Sharma R, et al. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice. J Immunol. 2017;198:2589-2601 pubmed publisher
Riether C, Schürch C, Bührer E, Hinterbrandner M, Huguenin A, Hoepner S, et al. CD70/CD27 signaling promotes blast stemness and is a viable therapeutic target in acute myeloid leukemia. J Exp Med. 2017;214:359-380 pubmed publisher
König C, Morch E, Eitner A, Möller C, Turnquist B, Schaible H, et al. Involvement of Spinal IL-6 Trans-Signaling in the Induction of Hyperexcitability of Deep Dorsal Horn Neurons by Spinal Tumor Necrosis Factor-Alpha. J Neurosci. 2016;36:9782-91 pubmed publisher
Sauter K, Pridans C, Sehgal A, Bain C, Scott C, Moffat L, et al. The MacBlue binary transgene (csf1r-gal4VP16/UAS-ECFP) provides a novel marker for visualisation of subsets of monocytes, macrophages and dendritic cells and responsiveness to CSF1 administration. PLoS ONE. 2014;9:e105429 pubmed publisher
Meyer zu Horste G, Mausberg A, Cordes S, El Haddad H, Partke H, Leussink V, et al. Thymic epithelium determines a spontaneous chronic neuritis in Icam1(tm1Jcgr)NOD mice. J Immunol. 2014;193:2678-90 pubmed publisher
Keating R, Hertz T, Wehenkel M, Harris T, Edwards B, McClaren J, et al. The kinase mTOR modulates the antibody response to provide cross-protective immunity to lethal infection with influenza virus. Nat Immunol. 2013;14:1266-76 pubmed publisher
Schwartz J, Barker J, Long M, Kaufman J, McCracken J, Allen L. Natural IgM mediates complement-dependent uptake of Francisella tularensis by human neutrophils via complement receptors 1 and 3 in nonimmune serum. J Immunol. 2012;189:3064-77 pubmed publisher
product information
Antigen :
IgG2b kappa
Apps. Abbrev. :
Flow Cytometry, Intracellular Staining for Flow Cytometry, Western Blotting, IP, ICC, Immunofluorescence Microscopy, IHC, FA
Cat # :
400637
Clone :
RTK4530
Item :
LEAF™ Purified Rat IgG2b, κ Isotype Ctrl
Isotype :
Rat IgG2b, κ
Size :
1 mg
Price (USD) :
175 USD
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
LEAF
Immunogen :
Trinitrophenol + KLH
Application Notes :
The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 400622) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400644) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA