Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Human GPI-APD Cocktail for WBC with Control Cells

391507

50 tests

849 USD

391507

Human GPI-APD Cocktail for WBC with Control Cells

Glycosylphosphatidylinositol (GPI)-anchored protein, control cells, Veri-Cells control

50 tests

849 USD

Human

SET

Veri-Cells™ Control Cell Protocol;Remove the kit from the fridge and allow it to warm to room temperature for 5 minutes. Keep unused kit components stored in the fridge.;Reconstitute 1 Veri-Cells™ Leukocytes vial with 325 µL of Buffer A Plus. Reconstitute a second Veri-Cells™ Leukocytes vial with 325 µL of GPI Buffer. Label each vial with the reconstitution buffer ("Buffer A Plus" and "GPI Buffer");Incubate the 2 vials at room temperature for 30 minutes.;Prepare two 5 mL FACS tubes (not provided). Label one "Buffer A Plus" and the other "GPI Buffer."Add 20 μL of the GPI-APD Cocktail for WBC to each tube;Add 50 μL of Veri-Cells™ Leukocytes reconstituted in Buffer A Plus or GPI Buffer to the appropriately labeled tube. Incubate the cells at room temperature and in the dark for about 15 minutes.;Add 2 mL of Cell Staining Buffer (Cat. No. 420201). Centrifuge at 350 x g for five minutes. Discard supernatant.;Wash one more time with 2 mL of Cell Staining Buffer.;Resuspend cell pellet in 0.2 - 0.4 mL of Cell Staining Buffer.;Combine the two tubes to make one tube of "Veri-Cells™ Control Cells."Acquire cells immediately (along with samples) on a flow cytometer or store at 4°C, in the dark, for no longer than 12 hours.