product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-human CD25
catalog :
356127
quantity :
25 tests
price :
124 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF647
clone name :
M-A251
reactivity :
human
application :
flow cytometry, mass cytometry
more info or order :
citations: 19
Published Application/Species/Sample/DilutionReference
  • mass cytometry; human; loading ...; fig 4e
  • flow cytometry; human; loading ...; fig 3b
Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
  • flow cytometry; human; loading ...
Vavassori V, Mercuri E, Marcovecchio G, Castiello M, Schiroli G, Albano L, et al. Modeling, optimization, and comparable efficacy of T cell and hematopoietic stem cell gene editing for treating hyper-IgM syndrome. EMBO Mol Med. 2021;13:e13545 pubmed publisher
  • flow cytometry; human; loading ...; fig s6-2
Kasatskaya S, Ladell K, Egorov E, Miners K, Davydov A, Metsger M, et al. Functionally specialized human CD4+ T-cell subsets express physicochemically distinct TCRs. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig 6c
Luo B, Zhan Y, Luo M, Dong H, Liu J, Lin Y, et al. Engineering of α-PD-1 antibody-expressing long-lived plasma cells by CRISPR/Cas9-mediated targeted gene integration. Cell Death Dis. 2020;11:973 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Thauland T, Pellerin L, Ohgami R, Bacchetta R, Butte M. Case Study: Mechanism for Increased Follicular Helper T Cell Development in Activated PI3K Delta Syndrome. Front Immunol. 2019;10:753 pubmed publisher
  • flow cytometry; human; loading ...; fig s2c
Ye Y, Liu M, Tang L, Du F, Liu Y, Hao P, et al. Iguratimod represses B cell terminal differentiation linked with the inhibition of PKC/EGR1 axis. Arthritis Res Ther. 2019;21:92 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Koppejan H, Jansen D, Hameetman M, Thomas R, Toes R, van Gaalen F. Altered composition and phenotype of mucosal-associated invariant T cells in early untreated rheumatoid arthritis. Arthritis Res Ther. 2019;21:3 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Tai Y, Lin L, Xing L, Cho S, Yu T, Acharya C, et al. APRIL signaling via TACI mediates immunosuppression by T regulatory cells in multiple myeloma: therapeutic implications. Leukemia. 2019;33:426-438 pubmed publisher
  • flow cytometry; human; loading ...; fig 1
Ondigo B, Ndombi E, Nicholson S, Oguso J, Carter J, Kittur N, et al. Functional Studies of T Regulatory Lymphocytes in Human Schistosomiasis in Western Kenya. Am J Trop Med Hyg. 2018;98:1770-1781 pubmed publisher
Thaker Y, Rivera I, Pedros C, Singh A, Rivero Nava L, Zhou H, et al. A Novel Affinity Engineered Anti-CD47 Antibody With Improved Therapeutic Index That Preserves Erythrocytes and Normal Immune Cells. Front Oncol. 2022;12:884196 pubmed publisher
Wang L, Verschuuren E, Paap D, Rondaan C, Raveling Eelsing E, Liu S, et al. Ageing of Immune System and Response to a Live-Attenuated Herpes Zoster Vaccine in Lung Transplant Candidates. Vaccines (Basel). 2021;9: pubmed publisher
Chen F, Li Z, Ma D, Yu Q. Small-molecule PD-L1 inhibitor BMS1166 abrogates the function of PD-L1 by blocking its ER export. Oncoimmunology. 2020;9:1831153 pubmed publisher
Wagner J, Rapsomaniki M, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer. Cell. 2019;177:1330-1345.e18 pubmed publisher
Baglaenko Y, Chang N, Johnson S, Hafiz W, Manion K, Ferri D, et al. The presence of anti-nuclear antibodies alone is associated with changes in B cell activation and T follicular helper cells similar to those in systemic autoimmune rheumatic disease. Arthritis Res Ther. 2018;20:264 pubmed publisher
Noto A, Procopio F, Banga R, Suffiotti M, Corpataux J, Cavassini M, et al. CD32+ and PD-1+ Lymph Node CD4 T Cells Support Persistent HIV-1 Transcription in Treated Aviremic Individuals. J Virol. 2018;92: pubmed publisher
Younan P, Iampietro M, Nishida A, Ramanathan P, Santos R, Dutta M, et al. Ebola Virus Binding to Tim-1 on T Lymphocytes Induces a Cytokine Storm. MBio. 2017;8: pubmed publisher
Salio M, Gasser O, González López C, Martens A, Veerapen N, Gileadi U, et al. Activation of Human Mucosal-Associated Invariant T Cells Induces CD40L-Dependent Maturation of Monocyte-Derived and Primary Dendritic Cells. J Immunol. 2017;199:2631-2638 pubmed publisher
Chevrier S, Levine J, Zanotelli V, Silina K, Schulz D, Bacac M, et al. An Immune Atlas of Clear Cell Renal Cell Carcinoma. Cell. 2017;169:736-749.e18 pubmed publisher
Wang Y, Ma C, Ling Y, Bousfiha A, Camcioglu Y, Jacquot S, et al. Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations. J Exp Med. 2016;213:2413-2435 pubmed
product information
Antigen :
CD25
Apps. Abbrev. :
FC, SB
Cat # :
356127
Clone :
M-A251
Item :
Alexa Fluor® 647 anti-human CD25
Isotype :
Mouse IgG1, κ
Other Names :
IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
Size :
25 tests
Price (USD) :
124 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A647
Immunogen :
Human PHA-induced lymphocyte cells
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections^1 and spatial biology (IBEX)^2,3.The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA