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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human TCR Vα7.2
catalog :
351702
quantity :
100 μg
price :
229 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3C10
reactivity :
human
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 18
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; human; loading ...; fig 1c5
Koppejan H, Jansen D, Hameetman M, Thomas R, Toes R, van Gaalen F. Altered composition and phenotype of mucosal-associated invariant T cells in early untreated rheumatoid arthritis. Arthritis Res Ther. 2019;21:3 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Dias J, Boulouis C, Gorin J, van den Biggelaar R, Lal K, Gibbs A, et al. The CD4-CD8- MAIT cell subpopulation is a functionally distinct subset developmentally related to the main CD8+ MAIT cell pool. Proc Natl Acad Sci U S A. 2018;115:E11513-E11522 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Salio M, Gasser O, González López C, Martens A, Veerapen N, Gileadi U, et al. Activation of Human Mucosal-Associated Invariant T Cells Induces CD40L-Dependent Maturation of Monocyte-Derived and Primary Dendritic Cells. J Immunol. 2017;199:2631-2638 pubmed publisher
  • flow cytometry; human; loading ...; fig 4c
Kim M, Yoo S, Kang S, Kwon J, Choi I, Lee C. TNF? and IL-1? in the synovial fluid facilitate mucosal-associated invariant T (MAIT) cell migration. Cytokine. 2017;99:91-98 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Dias J, Leeansyah E, Sandberg J. Multiple layers of heterogeneity and subset diversity in human MAIT cell responses to distinct microorganisms and to innate cytokines. Proc Natl Acad Sci U S A. 2017;114:E5434-E5443 pubmed publisher
  • flow cytometry; human; loading ...; fig 1d
Cottineau J, Kottemann M, Lach F, Kang Y, Vély F, Deenick E, et al. Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency. J Clin Invest. 2017;127:1991-2006 pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
Szabo P, Goswami A, Mazzuca D, Kim K, O Gorman D, Hess D, et al. Rapid and Rigorous IL-17A Production by a Distinct Subpopulation of Effector Memory T Lymphocytes Constitutes a Novel Mechanism of Toxic Shock Syndrome Immunopathology. J Immunol. 2017;198:2805-2818 pubmed publisher
  • flow cytometry; human; loading ...; fig s5a
Cheuk S, Schlums H, Gallais Sérézal I, Martini E, Chiang S, Marquardt N, et al. CD49a Expression Defines Tissue-Resident CD8+ T Cells Poised for Cytotoxic Function in Human Skin. Immunity. 2017;46:287-300 pubmed publisher
  • flow cytometry; human; loading ...; tbl 1
Sumatoh H, Teng K, Cheng Y, Newell E. Optimization of mass cytometry sample cryopreservation after staining. Cytometry A. 2017;91:48-61 pubmed publisher
  • flow cytometry; human; loading ...; fig e3c
Mitson Salazar A, Yin Y, Wansley D, Young M, Bolan H, Arceo S, et al. Hematopoietic prostaglandin D synthase defines a proeosinophilic pathogenic effector human T(H)2 cell subpopulation with enhanced function. J Allergy Clin Immunol. 2016;137:907-18.e9 pubmed publisher
  • flow cytometry; human; 1:20
Held K, Beltrán E, Moser M, Hohlfeld R, Dornmair K. T-cell receptor repertoire of human peripheral CD161hiTRAV1-2+ MAIT cells revealed by next generation sequencing and single cell analysis. Hum Immunol. 2015;76:607-14 pubmed publisher
  • flow cytometry; human; fig 1
Leeansyah E, Svärd J, Dias J, Buggert M, Nyström J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed publisher
  • flow cytometry; human; fig 2
Salerno Goncalves R, Rezwan T, Sztein M. B cells modulate mucosal associated invariant T cell immune responses. Front Immunol. 2014;4:511 pubmed publisher
Kaufmann M, Evans H, Schaupp A, Engler J, Kaur G, Willing A, et al. Identifying CNS-colonizing T cells as potential therapeutic targets to prevent progression of multiple sclerosis. Med (N Y). 2021;2:296-312.e8 pubmed publisher
Chng M, Lim M, Rouers A, Becht E, Lee B, Macary P, et al. Large-Scale HLA Tetramer Tracking of T Cells during Dengue Infection Reveals Broad Acute Activation and Differentiation into Two Memory Cell Fates. Immunity. 2019;51:1119-1135.e5 pubmed publisher
Nicol B, Salou M, Vogel I, Garcia A, Dugast E, Morille J, et al. An intermediate level of CD161 expression defines a novel activated, inflammatory, and pathogenic subset of CD8+ T cells involved in multiple sclerosis. J Autoimmun. 2018;88:61-74 pubmed publisher
Shaler C, Tun Abraham M, Skaro A, Khazaie K, Corbett A, Mele T, et al. Mucosa-associated invariant T cells infiltrate hepatic metastases in patients with colorectal carcinoma but are rendered dysfunctional within and adjacent to tumor microenvironment. Cancer Immunol Immunother. 2017;66:1563-1575 pubmed publisher
Wakao H, Yoshikiyo K, Koshimizu U, Furukawa T, Enomoto K, Matsunaga T, et al. Expansion of functional human mucosal-associated invariant T cells via reprogramming to pluripotency and redifferentiation. Cell Stem Cell. 2013;12:546-58 pubmed publisher
product information
Antigen :
TCR Valpha7.2
Apps. Abbrev. :
Flow Cytometry
Cat # :
351702
Clone :
3C10
Item :
Purified anti-human TCR Vα7.2
Isotype :
Mouse IgG1, κ
Other Names :
T cell antigen receptor Vα7.2
Size :
100 μg
Price (USD) :
229 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Recombinant TCR
Application Notes :
Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells. Additional reported applications (for the relevant formats) include: spatial biology (IBEX) 3,4 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA