PE/Dazzle™ 594 anti-human Ki-67 | BioLegend 350533 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Dazzle™ 594 anti-human Ki-67

catalog :

350533

quantity :

25 tests

price :

129 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Ki-67

reactivity :

human

application :

immunocytochemistry

more info or order :

BioLegend product webpage

citations: 3

Reference
Udayasuryan B, Ahmad R, Nguyen T, Uma xf1 a A, Mon xe9 t Roberts L, Sobol P, et al. Fusobacterium nucleatum induces proliferation and migration in pancreatic cancer cells through host autocrine and paracrine signaling. Sci Signal. 2022;15:eabn4948 pubmed publisher
Ramzy A, Thompson D, Ward Hartstonge K, Ivison S, Cook L, Garcia R, et al. Implanted pluripotent stem-cell-derived pancreatic endoderm cells secrete glucose-responsive C-peptide in patients with type 1 diabetes. Cell Stem Cell. 2021;28:2047-2061.e5 pubmed publisher
Jones R, Qian Y, Siciliano V, DiAndreth B, Huh J, Weiss R, et al. An endoribonuclease-based feedforward controller for decoupling resource-limited genetic modules in mammalian cells. Nat Commun. 2020;11:5690 pubmed publisher

product information

Antigen :

Ki-67

Apps. Abbrev. :

ICFC

Cat # :

350533

Clone :

Ki-67

Item :

PE/Dazzle™ 594 anti-human Ki-67

Isotype :

Mouse IgG1, κ

Other Names :

Antigen Ki-67

Size :

25 tests

Price (USD) :

129 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PE_DZL594

Immunogen :

Nuclei of the Hodgkin lymphoma cell line L428

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1, Western blotting^3, and immunofluorescence microscopy^4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10^6/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.;

more info or order :

BioLegend product webpage