PE/Cyanine7 anti-human Ki-67 | BioLegend 350525 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine7 anti-human Ki-67

catalog :

350525

quantity :

25 tests

price :

129 USD

clonality :

monoclonal

host :

mouse

conjugate :

PE/Cy7

clone name :

Ki-67

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 3b	Ashizawa T, Iizuka A, Nonomura C, Kondou R, Maeda C, Miyata H, et al. Antitumor Effect of Programmed Death-1 (PD-1) Blockade in Humanized the NOG-MHC Double Knockout Mouse. Clin Cancer Res. 2017;23:149-158 pubmed publisher
	Parajuli P, Rosati R, Mamdani H, Wright R, Hussain Z, Naeem A, et al. Senescence-associated secretory proteins induced in lung adenocarcinoma by extended treatment with dexamethasone enhance migration and activation of lymphocytes. Cancer Immunol Immunother. 2023;72:1273-1284 pubmed publisher
	Wild K, Smits M, Killmer S, Strohmeier S, Neumann Haefelin C, Bengsch B, et al. Pre-existing immunity and vaccine history determine hemagglutinin-specific CD4 T cell and IgG response following seasonal influenza vaccination. Nat Commun. 2021;12:6720 pubmed publisher
	Oberhardt V, Luxenburger H, Kemming J, Schulien I, Ciminski K, Giese S, et al. Rapid and stable mobilization of CD8+ T cells by SARS-CoV-2 mRNA vaccine. Nature. 2021;597:268-273 pubmed publisher
	Wong S, Oshansky C, Guo X, Ralston J, Wood T, Reynolds G, et al. Activated CD4+ T cells and CD14hiCD16+ monocytes correlate with antibody response following influenza virus infection in humans. Cell Rep Med. 2021;2:100237 pubmed publisher
	Thompson E, Cascino K, Ordonez A, Zhou W, Vaghasia A, Hamacher Brady A, et al. Metabolic programs define dysfunctional immune responses in severe COVID-19 patients. Cell Rep. 2021;34:108863 pubmed publisher
	Berezhnoy A, Sumrow B, Stahl K, Shah K, Liu D, Li J, et al. Development and Preliminary Clinical Activity of PD-1-Guided CTLA-4 Blocking Bispecific DART Molecule. Cell Rep Med. 2020;1:100163 pubmed publisher
	Wang J, Xu Y, Chen Z, Liang J, Lin Z, Liang H, et al. Liver Immune Profiling Reveals Pathogenesis and Therapeutics for Biliary Atresia. Cell. 2020;183:1867-1883.e26 pubmed publisher
	Rodriguez A, Zhang K, Färkkilä A, Filiatrault J, Yang C, Velázquez M, et al. MYC Promotes Bone Marrow Stem Cell Dysfunction in Fanconi Anemia. Cell Stem Cell. 2021;28:33-47.e8 pubmed publisher
	Turner J, Zhou J, Han J, Schmitz A, Rizk A, Alsoussi W, et al. Human germinal centres engage memory and naive B cells after influenza vaccination. Nature. 2020;586:127-132 pubmed publisher
	Satoh Takayama N, Kato T, Motomura Y, Kageyama T, Taguchi Atarashi N, Kinoshita Daitoku R, et al. Bacteria-Induced Group 2 Innate Lymphoid Cells in the Stomach Provide Immune Protection through Induction of IgA. Immunity. 2020;52:635-649.e4 pubmed publisher
	Zhong W, Xu M, Li C, Zhu B, Cao X, Li D, et al. ORP4L Extracts and Presents PIP2 from Plasma Membrane for PLCβ3 Catalysis: Targeting It Eradicates Leukemia Stem Cells. Cell Rep. 2019;26:2166-2177.e9 pubmed publisher
	Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed publisher

product information

Antigen :

Ki-67

Apps. Abbrev. :

ICFC

Cat # :

350525

Clone :

Ki-67

Item :

PE/Cyanine7 anti-human Ki-67

Isotype :

Mouse IgG1, κ

Other Names :

Antigen Ki-67

Size :

25 tests

Price (USD) :

129 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PE_CYANINE7

Immunogen :

Nuclei of the Hodgkin lymphoma cell line L428

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1, Western blotting^3, and immunofluorescence microscopy^4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10^6/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.;

more info or order :

BioLegend product webpage