Purified anti-human Ki-67 (Maxpar® Ready) | BioLegend 350523 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-human Ki-67 (Maxpar® Ready)

catalog :

350523

quantity :

100 μg

price :

171 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Ki-67

reactivity :

human, mouse

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:200; loading ...; fig s2c	Oberhardt V, Luxenburger H, Kemming J, Schulien I, Ciminski K, Giese S, et al. Rapid and stable mobilization of CD8+ T cells by SARS-CoV-2 mRNA vaccine. Nature. 2021;597:268-273 pubmed publisher
flow cytometry; human; 3:50; loading ...; fig 3b	Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
flow cytometry; human; 1:20; loading ...; fig 3a	Huang Y, Huang J, Huang Y, Gan L, Long L, Pu A, et al. TFRC promotes epithelial ovarian cancer cell proliferation and metastasis via up-regulation of AXIN2 expression. Am J Cancer Res. 2020;10:131-147 pubmed
flow cytometry; human; loading ...; fig 1b	Remmerswaal E, Hombrink P, Nota B, Pircher H, ten Berge I, van Lier R, et al. Expression of IL-7Rα and KLRG1 defines functionally distinct CD8+ T-cell populations in humans. Eur J Immunol. 2019;49:694-708 pubmed publisher
flow cytometry; human; loading ...; fig s6a	Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed publisher
flow cytometry; human; loading ...; fig 3a	Li M, Zhang W, Liu J, Li M, Zhang Y, Xiong Y, et al. Dynamic changes in the immunological characteristics of T lymphocytes in surviving patients with severe fever with thrombocytopenia syndrome (SFTS). Int J Infect Dis. 2018;70:72-80 pubmed publisher
flow cytometry; human; loading ...; fig 1b	Amodio D, Cotugno N, Macchiarulo G, Rocca S, Dimopoulos Y, Castrucci M, et al. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination. J Immunol. 2018;200:538-550 pubmed publisher
flow cytometry; human; loading ...; fig 2a	Vanegas N, Vernot J. Loss of quiescence and self-renewal capacity of hematopoietic stem cell in an in vitro leukemic niche. Exp Hematol Oncol. 2017;6:2 pubmed publisher
flow cytometry; human; loading ...; fig 3b	Ashizawa T, Iizuka A, Nonomura C, Kondou R, Maeda C, Miyata H, et al. Antitumor Effect of Programmed Death-1 (PD-1) Blockade in Humanized the NOG-MHC Double Knockout Mouse. Clin Cancer Res. 2017;23:149-158 pubmed publisher
flow cytometry; human; fig s2	Leeansyah E, SvÃ¤rd J, Dias J, Buggert M, NystrÃ¶m J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed publisher
flow cytometry; mouse; fig 4	Sakamoto H, Takeda N, Arai F, Hosokawa K, García P, Suda T, et al. Determining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Stem Cells. 2015;33:479-90 pubmed publisher
	Gadalla R, Boukhaled G, Brooks D, Wang B. Mass cytometry immunostaining protocol for multiplexing clinical samples. STAR Protoc. 2022;3:101643 pubmed publisher
	. A blood atlas of COVID-19 defines hallmarks of disease severity and specificity. Cell. 2022;185:916-938.e58 pubmed publisher
	Senosain M, Zou Y, Novitskaya T, Vasiukov G, Balar A, Rowe D, et al. HLA-DR cancer cells expression correlates with T cell infiltration and is enriched in lung adenocarcinoma with indolent behavior. Sci Rep. 2021;11:14424 pubmed publisher
	Roussel M, Ferrant J, Reizine F, Le Gallou S, Dulong J, Carl S, et al. Comparative immune profiling of acute respiratory distress syndrome patients with or without SARS-CoV-2 infection. Cell Rep Med. 2021;2:100291 pubmed publisher
	Loo Yau H, Bell E, Ettayebi I, de Almeida F, Boukhaled G, Shen S, et al. DNA hypomethylating agents increase activation and cytolytic activity of CD8+ T cells. Mol Cell. 2021;81:1469-1483.e8 pubmed publisher
	Schulte Schrepping J, Reusch N, Paclik D, Baßler K, Schlickeiser S, Zhang B, et al. Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment. Cell. 2020;: pubmed publisher
	Stras S, Werner L, Toothaker J, Olaloye O, Oldham A, McCourt C, et al. Maturation of the Human Intestinal Immune System Occurs Early in Fetal Development. Dev Cell. 2019;51:357-373.e5 pubmed publisher
	Jordan S, Tung N, Casanova Acebes M, Chang C, Cantoni C, Zhang D, et al. Dietary Intake Regulates the Circulating Inflammatory Monocyte Pool. Cell. 2019;178:1102-1114.e17 pubmed publisher
	Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed publisher

product information

Antigen :

Ki-67

Apps. Abbrev. :

ICFC, CyTOF®

Cat # :

350523

Clone :

Ki-67

Item :

Purified anti-human Ki-67 (Maxpar® Ready)

Isotype :

Mouse IgG1, κ

Other Names :

Antigen Ki-67

Size :

100 μg

Price (USD) :

171 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PUREMAX

Immunogen :

Nuclei of the Hodgkin lymphoma cell line L428

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1, Western blotting^3, and immunofluorescence microscopy^4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10^6/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.;

more info or order :

BioLegend product webpage