Brilliant Violet 605™ anti-human Ki-67 | BioLegend 350521 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 605™ anti-human Ki-67

catalog :

350521

quantity :

25 tests

price :

242 USD

clonality :

monoclonal

host :

mouse

conjugate :

BV605

clone name :

Ki-67

reactivity :

human

application :

immunocytochemistry

more info or order :

BioLegend product webpage

citations: 10

Reference
Eser T, Baranov O, Huth M, Ahmed M, De xe1 k F, Held K, et al. Nucleocapsid-specific T cell responses associate with control of SARS-CoV-2 in the upper airways before seroconversion. Nat Commun. 2023;14:2952 pubmed publisher
Alam A, Levanduski E, Denz P, Villavicencio H, Bhatta M, Alhorebi L, et al. Fungal mycobiome drives IL-33 secretion and type 2 immunity in pancreatic cancer. Cancer Cell. 2022;40:153-167.e11 pubmed publisher
Den Braanker H, Bongenaar M, Lubberts E. How to Prepare Spectral Flow Cytometry Datasets for High Dimensional Data Analysis: A Practical Workflow. Front Immunol. 2021;12:768113 pubmed publisher
Huanosta Murillo E, Alc xe1 ntara Hern xe1 ndez M, Hern xe1 ndez Rico B, Victoria Acosta G, Miranda Cruz P, Dom xed nguez G xf3 mez M, et al. NLRP3 Regulates IL-4 Expression in TOX+ CD4+ T Cells of Cutaneous T Cell Lymphoma to Potentially Promote Disease Progression. Front Immunol. 2021;12:668369 pubmed publisher
Caduff N, McHugh D, Rieble L, Forconi C, Ong echa J, Oluoch P, et al. KSHV infection drives poorly cytotoxic CD56-negative natural killer cell differentiation in vivo upon KSHV/EBV dual infection. Cell Rep. 2021;35:109056 pubmed publisher
Heyde A, Rohde D, McAlpine C, Zhang S, Hoyer F, Gerold J, et al. Increased stem cell proliferation in atherosclerosis accelerates clonal hematopoiesis. Cell. 2021;184:1348-1361.e22 pubmed publisher
Pagel J, Twisselmann N, Rausch T, Waschina S, Hartz A, Steinbeis M, et al. Increased Regulatory T Cells Precede the Development of Bronchopulmonary Dysplasia in Preterm Infants. Front Immunol. 2020;11:565257 pubmed publisher
Oh D, Kwek S, Raju S, Li T, McCarthy E, Chow E, et al. Intratumoral CD4+ T Cells Mediate Anti-tumor Cytotoxicity in Human Bladder Cancer. Cell. 2020;181:1612-1625.e13 pubmed publisher
Li R, Liu H, Cao Y, Wang J, Chen Y, Qi Y, et al. Identification and validation of an immunogenic subtype of gastric cancer with abundant intratumoural CD103+CD8+ T cells conferring favourable prognosis. Br J Cancer. 2020;122:1525-1534 pubmed publisher
Ligorio M, Sil S, Malagon Lopez J, Nieman L, Misale S, Di Pilato M, et al. Stromal Microenvironment Shapes the Intratumoral Architecture of Pancreatic Cancer. Cell. 2019;: pubmed publisher

product information

Antigen :

Ki-67

Apps. Abbrev. :

ICFC

Cat # :

350521

Clone :

Ki-67

Item :

Brilliant Violet 605™ anti-human Ki-67

Isotype :

Mouse IgG1, κ

Other Names :

Antigen Ki-67

Size :

25 tests

Price (USD) :

242 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

BV605

Immunogen :

Nuclei of the Hodgkin lymphoma cell line L428

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1, Western blotting^3, and immunofluorescence microscopy^4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10^6/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.;

more info or order :

BioLegend product webpage