PerCP/Cyanine5.5 anti-human Ki-67 | BioLegend 350519 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PerCP/Cyanine5.5 anti-human Ki-67

catalog :

350519

quantity :

25 tests

price :

136 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Ki-67

reactivity :

human

application :

immunocytochemistry

more info or order :

BioLegend product webpage

citations: 6

Reference
Manolakou T, Kaltezioti V, Prakoura N, Kavvadas P, Reichelt Wurm S, Gakiopoulou H, et al. Down-regulation of human long non-coding RNA LINC01187 is associated with nephropathies. J Cell Mol Med. 2023;27:1192-1205 pubmed publisher
Wang J, Man Q, Zhong N, Wang H, Zhang C, Li S, et al. ENO1 Binds to ApoC3 and Impairs the Proliferation of T Cells via IL-8/STAT3 Pathway in OSCC. Int J Mol Sci. 2022;23: pubmed publisher
Zhu S, Zhang C, Sun Q, Wang Y, Yu W, Wei F, et al. Trained Immunity of IL-12-, IL-15-, and IL-18-Induced CD3+CD56+ NKT-Like Cells. J Oncol. 2022;2022:8724933 pubmed publisher
Wang Y, Qiu J, Qu X, Peng J, Lu C, Zhang M, et al. Accumulation of dysfunctional tumor-infiltrating PD-1+ DCs links PD-1/PD-L1 blockade immunotherapeutic response in cervical cancer. Oncoimmunology. 2022;11:2034257 pubmed publisher
Leylek R, Alcántara Hernández M, Granja J, Chavez M, Perez K, Diaz O, et al. Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity. Cell Rep. 2020;32:108180 pubmed publisher
Yang H, Wang C, Lai Z, Yang S, Zheng Z, Shi J, et al. Decidual stromal cells maintain decidual macrophage homeostasis by secreting IL-24 in early pregnancy. Am J Reprod Immunol. 2020;84:e13261 pubmed publisher

product information

Antigen :

Ki-67

Apps. Abbrev. :

ICFC

Cat # :

350519

Clone :

Ki-67

Item :

PerCP/Cyanine5.5 anti-human Ki-67

Isotype :

Mouse IgG1, κ

Other Names :

Antigen Ki-67

Size :

25 tests

Price (USD) :

136 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PERCP_CYANINE5.5

Immunogen :

Nuclei of the Hodgkin lymphoma cell line L428

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1, Western blotting^3, and immunofluorescence microscopy^4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10^6/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.;

more info or order :

BioLegend product webpage