Brilliant Violet 711™ anti-human Ki-67 | BioLegend 350516 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 711™ anti-human Ki-67

catalog :

350516

quantity :

100 tests

price :

446 USD

clonality :

monoclonal

host :

mouse

conjugate :

BV711

clone name :

Ki-67

reactivity :

human, mouse

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:200; loading ...; fig s2c	Oberhardt V, Luxenburger H, Kemming J, Schulien I, Ciminski K, Giese S, et al. Rapid and stable mobilization of CD8+ T cells by SARS-CoV-2 mRNA vaccine. Nature. 2021;597:268-273 pubmed publisher
flow cytometry; human; loading ...; fig 4s1	Pallikkuth S, Chaudhury S, Lu P, Pan L, Jongert E, Wille Reece U, et al. A delayed fractionated dose RTS,S AS01 vaccine regimen mediates protection via improved T follicular helper and B cell responses. elife. 2020;9: pubmed publisher
flow cytometry; human; loading ...; fig 1b	Remmerswaal E, Hombrink P, Nota B, Pircher H, ten Berge I, van Lier R, et al. Expression of IL-7Rα and KLRG1 defines functionally distinct CD8+ T-cell populations in humans. Eur J Immunol. 2019;49:694-708 pubmed publisher
flow cytometry; mouse; fig 4	Sakamoto H, Takeda N, Arai F, Hosokawa K, García P, Suda T, et al. Determining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Stem Cells. 2015;33:479-90 pubmed publisher
	Myers J, Schirm D, Bendzick L, Hopps R, Selleck C, Hinderlie P, et al. Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion. JCI Insight. 2022;7: pubmed publisher
	Reuschl A, Mesner D, Shivkumar M, Whelan M, Pallett L, Guerra Assun xe7 xe3 o J, et al. HIV-1 Vpr drives a tissue residency-like phenotype during selective infection of resting memory T cells. Cell Rep. 2022;39:110650 pubmed publisher
	Mudd P, Minervina A, Pogorelyy M, Turner J, Kim W, Kalaidina E, et al. SARS-CoV-2 mRNA vaccination elicits a robust and persistent T follicular helper cell response in humans. Cell. 2022;185:603-613.e15 pubmed publisher
	Lam A, Lin D, Gillies J, Uday P, Pesenacker A, Kobor M, et al. Optimized CRISPR-mediated gene knockin reveals FOXP3-independent maintenance of human Treg identity. Cell Rep. 2021;36:109494 pubmed publisher
	Turner J, O Halloran J, Kalaidina E, Kim W, Schmitz A, Zhou J, et al. SARS-CoV-2 mRNA vaccines induce persistent human germinal centre responses. Nature. 2021;596:109-113 pubmed publisher
	Turner J, Kim W, Kalaidina E, Goss C, Rauseo A, Schmitz A, et al. SARS-CoV-2 infection induces long-lived bone marrow plasma cells in humans. Nature. 2021;595:421-425 pubmed publisher
	Caduff N, McHugh D, Rieble L, Forconi C, Ong echa J, Oluoch P, et al. KSHV infection drives poorly cytotoxic CD56-negative natural killer cell differentiation in vivo upon KSHV/EBV dual infection. Cell Rep. 2021;35:109056 pubmed publisher
	Schreurs R, Sagebiel A, Steinert F, Highton A, Klarenbeek P, Drewniak A, et al. Intestinal CD8+ T cell responses are abundantly induced early in human development but show impaired cytotoxic effector capacities. Mucosal Immunol. 2021;14:605-614 pubmed publisher
	Zhang X, Lu X, Cheung A, Zhang Q, Liu Z, Li Z, et al. Analysis of the Characteristics of TIGIT-Expressing CD3-CD56+NK Cells in Controlling Different Stages of HIV-1 Infection. Front Immunol. 2021;12:602492 pubmed publisher
	Giron L, Colomb F, Papasavvas E, Azzoni L, Yin X, Fair M, et al. Interferon-α alters host glycosylation machinery during treated HIV infection. EBioMedicine. 2020;59:102945 pubmed publisher
	Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
	Jimeno R, Leceta J, GarÃn M, Ortiz A, Mellado M, RodrÃguez Frade J, et al. Th17 polarization of memory Th cells in early arthritis: the vasoactive intestinal peptide effect. J Leukoc Biol. 2015;98:257-69 pubmed publisher
	van Aalderen M, Remmerswaal E, Verstegen N, Hombrink P, ten Brinke A, Pircher H, et al. Infection history determines the differentiation state of human CD8+ T cells. J Virol. 2015;89:5110-23 pubmed publisher
	Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed publisher

product information

Antigen :

Ki-67

Apps. Abbrev. :

ICFC

Cat # :

350516

Clone :

Ki-67

Item :

Brilliant Violet 711™ anti-human Ki-67

Isotype :

Mouse IgG1, κ

Other Names :

Antigen Ki-67

Size :

100 tests

Price (USD) :

446 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

BV711

Immunogen :

Nuclei of the Hodgkin lymphoma cell line L428

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1, Western blotting^3, and immunofluorescence microscopy^4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10^6/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.;

more info or order :

BioLegend product webpage