product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-human Ki-67
catalog :
350514
quantity :
100 tests
price :
326 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
Ki-67
reactivity :
human
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 22
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2a
Vanegas N, Vernot J. Loss of quiescence and self-renewal capacity of hematopoietic stem cell in an in vitro leukemic niche. Exp Hematol Oncol. 2017;6:2 pubmed publisher
Jiang D, Huang H, Qin H, Tang K, Shi X, Zhu T, et al. Chimeric antigen receptor T cells targeting FcRH5 provide robust tumour-specific responses in murine xenograft models of multiple myeloma. Nat Commun. 2023;14:3642 pubmed publisher
Wang Z, Shen J, Ye K, Zhao J, Huang S, He S, et al. Neutrophil-Derived IL-6 Potentially Drives Ferroptosis Resistance in B Cells in Lupus Kidney. Mediators Inflamm. 2023;2023:9810733 pubmed publisher
Morris V, Wang D, Li Z, Marion W, Hughes T, Sousa P, et al. Hypoxic, glycolytic metabolism is a vulnerability of B-acute lymphoblastic leukemia-initiating cells. Cell Rep. 2022;39:110752 pubmed publisher
Xie G, Zheng X, Zheng Z, Wu R, Yao Z, Huang W, et al. The ceRNA PVT1 inhibits proliferation of ccRCC cells by sponging miR-328-3p to elevate FAM193B expression. Aging (Albany NY). 2021;13:21712-21728 pubmed publisher
Chugh R, Park H, El Andaloussi A, Elsharoud A, Esfandyari S, Ulin M, et al. Mesenchymal stem cell therapy ameliorates metabolic dysfunction and restores fertility in a PCOS mouse model through interleukin-10. Stem Cell Res Ther. 2021;12:388 pubmed publisher
Scherpenisse M, Kootstra N, Bakker M, Berkhout B, Pasternak A. Cell-Associated HIV-1 Unspliced-to-Multiply-Spliced RNA Ratio at 12 Weeks of ART Predicts Immune Reconstitution on Therapy. MBio. 2021;12: pubmed publisher
Park H, Chugh R, El Andaloussi A, Hobeika E, Esfandyari S, Elsharoud A, et al. Human BM-MSC secretome enhances human granulosa cell proliferation and steroidogenesis and restores ovarian function in primary ovarian insufficiency mouse model. Sci Rep. 2021;11:4525 pubmed publisher
Zhang D, Reyes R, Osta E, Kari S, Gupta H, Padrón A, et al. Bladder cancer cell-intrinsic PD-L1 signals promote mTOR and autophagy activation that can be inhibited to improve cytotoxic chemotherapy. Cancer Med. 2021;10:2137-2152 pubmed publisher
Dubuisson A, Fahrner J, Goubet A, Terrisse S, Voisin N, Bayard C, et al. Immunodynamics of explanted human tumors for immuno-oncology. EMBO Mol Med. 2021;13:e12850 pubmed publisher
Jung Y, Cackowski F, Yumoto K, Decker A, Wang Y, Hotchkin M, et al. Abscisic acid regulates dormancy of prostate cancer disseminated tumor cells in the bone marrow. Neoplasia. 2021;23:102-111 pubmed publisher
Zhou W, Wang H, Zhang J, Dai H, Yao Z, Zheng Z, et al. NEAT1/miR-200b-3p/SMAD2 axis promotes progression of melanoma. Aging (Albany NY). 2020;12:22759-22775 pubmed publisher
Evans R, Antonelou M, Sathiananthamoorthy S, Rega M, Henderson S, Ceron Gutierrez L, et al. Inherited salt-losing tubulopathies are associated with immunodeficiency due to impaired IL-17 responses. Nat Commun. 2020;11:4368 pubmed publisher
Landgraf K, Williams S, Steiger D, Gebhart D, Lok S, Martin D, et al. convertibleCARs: A chimeric antigen receptor system for flexible control of activity and antigen targeting. Commun Biol. 2020;3:296 pubmed publisher
Kobayashi Y, Yamada D, Kawai T, Sato Y, Teshima T, Yamada Y, et al. Different immunological effects of the molecular targeted agents sunitinib, everolimus and temsirolimus in patients with renal cell carcinoma. Int J Oncol. 2020;56:999-1013 pubmed publisher
Wang D, Liu N, Xie Y, Song B, Kong S, Sun X. Different culture method changing CD105 expression in amniotic fluid MSCs without affecting differentiation ability or immune function. J Cell Mol Med. 2020;24:4212-4222 pubmed publisher
Sontheimer Phelps A, Chou D, Tovaglieri A, Ferrante T, Duckworth T, Fadel C, et al. Human Colon-on-a-Chip Enables Continuous In Vitro Analysis of Colon Mucus Layer Accumulation and Physiology. Cell Mol Gastroenterol Hepatol. 2020;9:507-526 pubmed publisher
Wu K, Mu X, Jiang J, Tan M, Wang R, Zhou W, et al. miRNA‑26a‑5p and miR‑26b‑5p inhibit the proliferation of bladder cancer cells by regulating PDCD10. Oncol Rep. 2018;40:3523-3532 pubmed publisher
Yumoto K, Eber M, Wang J, Cackowski F, Decker A, Lee E, et al. Axl is required for TGF-β2-induced dormancy of prostate cancer cells in the bone marrow. Sci Rep. 2016;6:36520 pubmed publisher
Nodomi S, Umeda K, Saida S, Kinehara T, Hamabata T, Daifu T, et al. CD146 is a novel marker for highly tumorigenic cells and a potential therapeutic target in malignant rhabdoid tumor. Oncogene. 2016;35:5317-5327 pubmed publisher
Maine C, Marquardt K, Cheung J, Sherman L. PTPN22 controls the germinal center by influencing the numbers and activity of T follicular helper cells. J Immunol. 2014;192:1415-24 pubmed publisher
Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed publisher
product information
Antigen :
Ki-67
Apps. Abbrev. :
ICFC
Cat # :
350514
Clone :
Ki-67
Item :
APC anti-human Ki-67
Isotype :
Mouse IgG1, κ
Other Names :
Antigen Ki-67
Size :
100 tests
Price (USD) :
326 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC
Immunogen :
Nuclei of the Hodgkin lymphoma cell line L428
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1, Western blotting^3, and immunofluorescence microscopy^4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10^6/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA