Alexa Fluor® 647 anti-human Ki-67 | BioLegend 350509 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Alexa Fluor® 647 anti-human Ki-67

catalog :

350509

quantity :

25 tests

price :

136 USD

clonality :

monoclonal

host :

mouse

conjugate :

AF647

clone name :

Ki-67

reactivity :

human, mouse

application :

immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

BioLegend product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:200; loading ...; fig s2c	Oberhardt V, Luxenburger H, Kemming J, Schulien I, Ciminski K, Giese S, et al. Rapid and stable mobilization of CD8+ T cells by SARS-CoV-2 mRNA vaccine. Nature. 2021;597:268-273 pubmed publisher
flow cytometry; human; 3:50; loading ...; fig 3b	Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
flow cytometry; human; 1:20; loading ...; fig 3a	Huang Y, Huang J, Huang Y, Gan L, Long L, Pu A, et al. TFRC promotes epithelial ovarian cancer cell proliferation and metastasis via up-regulation of AXIN2 expression. Am J Cancer Res. 2020;10:131-147 pubmed
flow cytometry; human; loading ...; fig 1b	Remmerswaal E, Hombrink P, Nota B, Pircher H, ten Berge I, van Lier R, et al. Expression of IL-7Rα and KLRG1 defines functionally distinct CD8+ T-cell populations in humans. Eur J Immunol. 2019;49:694-708 pubmed publisher
flow cytometry; human; loading ...; fig s6a	Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed publisher
immunohistochemistry - paraffin section; human immunohistochemistry; human	Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
flow cytometry; human; loading ...; fig 3a	Li M, Zhang W, Liu J, Li M, Zhang Y, Xiong Y, et al. Dynamic changes in the immunological characteristics of T lymphocytes in surviving patients with severe fever with thrombocytopenia syndrome (SFTS). Int J Infect Dis. 2018;70:72-80 pubmed publisher
flow cytometry; human; loading ...; fig 1b	Amodio D, Cotugno N, Macchiarulo G, Rocca S, Dimopoulos Y, Castrucci M, et al. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination. J Immunol. 2018;200:538-550 pubmed publisher
	Schulz E, Schumann M, Schneemann M, Dony V, Fromm A, Nagel O, et al. Escherichia coli Alpha-Hemolysin HlyA Induces Host Cell Polarity Changes, Epithelial Barrier Dysfunction and Cell Detachment in Human Colon Carcinoma Caco-2 Cell Model via PTEN-Dependent Dysregulation of Cell Junctions. Toxins (Basel). 2021;13: pubmed publisher
	Joshi S, Nechiporuk T, Bottomly D, Piehowski P, Reisz J, Pittsenbarger J, et al. The AML microenvironment catalyzes a stepwise evolution to gilteritinib resistance. Cancer Cell. 2021;39:999-1014.e8 pubmed publisher
	Yokomizo Nakano T, Kubota S, Bai J, Hamashima A, Morii M, Sun Y, et al. Overexpression of RUNX3 Represses RUNX1 to Drive Transformation of Myelodysplastic Syndrome. Cancer Res. 2020;80:2523-2536 pubmed publisher
	Lindesmith L, Brewer Jensen P, Mallory M, Jensen K, Yount B, Costantini V, et al. Virus-Host Interactions Between Nonsecretors and Human Norovirus. Cell Mol Gastroenterol Hepatol. 2020;10:245-267 pubmed publisher
	Kathiriya J, Brumwell A, Jackson J, Tang X, Chapman H. Distinct Airway Epithelial Stem Cells Hide among Club Cells but Mobilize to Promote Alveolar Regeneration. Cell Stem Cell. 2020;26:346-358.e4 pubmed publisher
	Pei S, Minhajuddin M, Adane B, Khan N, Stevens B, Mack S, et al. AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells. Cell Stem Cell. 2018;23:86-100.e6 pubmed publisher
	Ong S, Hadadi E, Dang T, Yeap W, Tan C, Ng T, et al. The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence. Cell Death Dis. 2018;9:266 pubmed publisher
	Trabanelli S, Chevalier M, Martinez Usatorre A, Gomez Cadena A, Salomé B, Lecciso M, et al. Tumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axis. Nat Commun. 2017;8:593 pubmed publisher
	Watanabe N, Bajgain P, Sukumaran S, Ansari S, Heslop H, Rooney C, et al. Fine-tuning the CAR spacer improves T-cell potency. Oncoimmunology. 2016;5:e1253656 pubmed publisher
	Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed publisher

product information

Antigen :

Ki-67

Apps. Abbrev. :

ICFC, ICC

Cat # :

350509

Clone :

Ki-67

Item :

Alexa Fluor® 647 anti-human Ki-67

Isotype :

Mouse IgG1, κ

Other Names :

Antigen Ki-67

Size :

25 tests

Price (USD) :

136 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

A647

Immunogen :

Nuclei of the Hodgkin lymphoma cell line L428

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1, Western blotting^3, and immunofluorescence microscopy^4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10^6/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.;

more info or order :

BioLegend product webpage