Brilliant Violet 421™ anti-human Ki-67 | BioLegend 350505 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-human Ki-67

catalog :

350505

quantity :

25 tests

price :

209 USD

clonality :

monoclonal

host :

mouse

conjugate :

BV421

clone name :

Ki-67

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 3:50; loading ...; fig 3b	Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
flow cytometry; human; fig s2	Leeansyah E, SvÃ¤rd J, Dias J, Buggert M, NystrÃ¶m J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed publisher
	Wang F, Long J, Li L, Wu Z, Da T, Wang X, et al. Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer. Sci Adv. 2023;9:eadf5464 pubmed publisher
	Gkika E, Firat E, Adebahr S, Graf E, Popp I, Radicioni G, et al. Systemic immune modulation by stereotactic radiotherapy in early-stage lung cancer. NPJ Precis Oncol. 2023;7:24 pubmed publisher
	Wang Y, Wang Y, Liu W, Ding L, Zhang X, Wang B, et al. TIM-4 orchestrates mitochondrial homeostasis to promote lung cancer progression via ANXA2/PI3K/AKT/OPA1 axis. Cell Death Dis. 2023;14:141 pubmed publisher
	Ackerley C, Smith S, Murray P, Amancha P, Arthur R, Zhu Z, et al. The rectal mucosal immune environment and HIV susceptibility among young men who have sex with men. Front Immunol. 2022;13:972170 pubmed publisher
	Izmirly A, Pelletier A, Connors J, Taramangalam B, Alturki S, Gordon E, et al. Pre-vaccination frequency of circulatory Tfh is associated with robust immune response to TV003 dengue vaccine. PLoS Pathog. 2022;18:e1009903 pubmed publisher
	Cillo A, Somasundaram A, Shan F, Cardello C, Workman C, Kitsios G, et al. People critically ill with COVID-19 exhibit peripheral immune profiles predictive of mortality and reflective of SARS-CoV-2 lung viral burden. Cell Rep Med. 2021;2:100476 pubmed publisher
	Fu Y, Zhang Z, Yang Z, Jiang Y, Han X, Xu J, et al. CD27-CD38+ B cells accumulated in early HIV infection exhibit transitional profile and promote HIV disease progression. Cell Rep. 2021;36:109344 pubmed publisher
	Taylor H, Calantone N, Lichon D, Hudson H, Clerc I, Campbell E, et al. mTOR Overcomes Multiple Metabolic Restrictions to Enable HIV-1 Reverse Transcription and Intracellular Transport. Cell Rep. 2020;31:107810 pubmed publisher
	Singh M, Jackson K, Wang J, Schofield P, Field M, Koppstein D, et al. Lymphoma Driver Mutations in the Pathogenic Evolution of an Iconic Human Autoantibody. Cell. 2020;180:878-894.e19 pubmed publisher
	Sprouse M, Welte T, Boral D, Liu H, Yin W, Vishnoi M, et al. PMN-MDSCs Enhance CTC Metastatic Properties through Reciprocal Interactions via ROS/Notch/Nodal Signaling. Int J Mol Sci. 2019;20: pubmed publisher
	Vishnoi M, Boral D, Liu H, Sprouse M, Yin W, Goswami Sewell D, et al. Targeting USP7 Identifies a Metastasis-Competent State within Bone Marrow-Resident Melanoma CTCs. Cancer Res. 2018;78:5349-5362 pubmed publisher
	Siriwon N, Kim Y, Siegler E, Chen X, Rohrs J, Liu Y, et al. CAR-T Cells Surface-Engineered with Drug-Encapsulated Nanoparticles Can Ameliorate Intratumoral T-cell Hypofunction. Cancer Immunol Res. 2018;6:812-824 pubmed publisher
	Chierico L, Rizzello L, Guan L, Joseph A, Lewis A, Battaglia G. The role of the two splice variants and extranuclear pathway on Ki-67 regulation in non-cancer and cancer cells. PLoS ONE. 2017;12:e0171815 pubmed publisher
	Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed publisher

product information

Antigen :

Ki-67

Apps. Abbrev. :

ICFC, ICC

Cat # :

350505

Clone :

Ki-67

Item :

Brilliant Violet 421™ anti-human Ki-67

Isotype :

Mouse IgG1, κ

Other Names :

Antigen Ki-67

Size :

25 tests

Price (USD) :

209 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

BV421

Immunogen :

Nuclei of the Hodgkin lymphoma cell line L428

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1, Western blotting^3, and immunofluorescence microscopy^4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10^6/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.;

more info or order :

BioLegend product webpage