product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-human Ki-67
catalog :
350504
quantity :
100 tests
price :
347 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
Ki-67
reactivity :
human
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 34
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
Brittan M, Barr L, Anderson N, Morris A, Duffin R, Marwick J, et al. Functional characterisation of human pulmonary monocyte-like cells in lipopolysaccharide-mediated acute lung inflammation. J Inflamm (Lond). 2014;11:9 pubmed publisher
Perveen R, Ozaki I, Manirujjaman M, Mine K, Murata Y, Tanaka K, et al. Induction of premature senescence and a less-fibrogenic phenotype by programmed cell death 4 knockdown in the human hepatic stellate cell line Lieming Xu-2. Hum Cell. 2023;36:583-601 pubmed publisher
Jo S, Lee S, Park J, Nam B, Kim H, Youn J, et al. Platelet-Derived Growth Factor B Is a Key Element in the Pathological Bone Formation of Ankylosing Spondylitis. J Bone Miner Res. 2023;38:300-312 pubmed publisher
Sharma A, Shafer D, Netting D, Tyagi M. Cocaine sensitizes the CD4+ T cells for HIV infection by co-stimulating NFAT and AP-1. iScience. 2022;25:105651 pubmed publisher
Abe I, Oguri Y, Verkerke A, Monteiro L, Knuth C, Auger C, et al. Lipolysis-derived linoleic acid drives beige fat progenitor cell proliferation. Dev Cell. 2022;57:2623-2637.e8 pubmed publisher
Jung S, Jung J, Noh J, Kim W, Yoon S, Jung J, et al. The generation of stem cell-like memory cells early after BNT162b2 vaccination is associated with durability of memory CD8+ T cell responses. Cell Rep. 2022;40:111138 pubmed publisher
Wang L, Chang C, Cheng C, Liang Y, Pei D, Sun J, et al. MCRS1 Expression Regulates Tumor Activity and Affects Survival Probability of Patients with Gastric Cancer. Diagnostics (Basel). 2022;12: pubmed publisher
Chang H, Sun J, Cheng C, Liang Y, Chen Y. High Mobility Group A 1 Expression as a Poor Prognostic Marker Associated with Tumor Invasiveness in Gastric Cancer. Life (Basel). 2022;12: pubmed publisher
Reuschl A, Mesner D, Shivkumar M, Whelan M, Pallett L, Guerra Assun xe7 xe3 o J, et al. HIV-1 Vpr drives a tissue residency-like phenotype during selective infection of resting memory T cells. Cell Rep. 2022;39:110650 pubmed publisher
Chen M, Li Y, Wu Y, Xie S, Ma J, Yue J, et al. Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer. Cancers (Basel). 2021;13: pubmed publisher
Szodoray P, Andersen T, Heinzelbecker J, Imbery J, Huszthy P, Stanford S, et al. Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity. Cell Rep. 2021;36:109525 pubmed publisher
Seo I, Eun H, Kim J, Lee H, Jeong S, Choi S, et al. IL-15 enhances CCR5-mediated migration of memory CD8+ T cells by upregulating CCR5 expression in the absence of TCR stimulation. Cell Rep. 2021;36:109438 pubmed publisher
Su Y, Chen D, Yuan D, Lausted C, Choi J, Dai C, et al. Multi-Omics Resolves a Sharp Disease-State Shift between Mild and Moderate COVID-19. Cell. 2020;183:1479-1495.e20 pubmed publisher
Hu X, Liu Y, Zhang X, Kong D, Kong J, Zhao D, et al. The anti-B7-H4 checkpoint synergizes trastuzumab treatment to promote phagocytosis and eradicate breast cancer. Neoplasia. 2020;22:539-553 pubmed publisher
Hübner M, Moellhoff N, Effinger D, Hinske C, Hirschberger S, Wu T, et al. MicroRNA-93 acts as an "anti-inflammatory tumor suppressor" in glioblastoma. Neurooncol Adv. 2020;2:vdaa047 pubmed publisher
Oguri Y, Shinoda K, Kim H, Alba D, Bolus W, Wang Q, et al. CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK Signaling. Cell. 2020;: pubmed publisher
Li M, Xiong Y, Li M, Zhang W, Liu J, Zhang Y, et al. Depletion but Activation of CD56dimCD16+ NK Cells in Acute Infection with Severe Fever with Thrombocytopenia Syndrome Virus. Virol Sin. 2020;35:588-598 pubmed publisher
Wangler S, Peroglio M, Menzel U, Benneker L, Haglund L, Sakai D, et al. Mesenchymal Stem Cell Homing Into Intervertebral Discs Enhances the Tie2-positive Progenitor Cell Population, Prevents Cell Death, and Induces a Proliferative Response. Spine (Phila Pa 1976). 2019;44:1613-1622 pubmed publisher
Makabe K, Sugita S, Hono A, Kamao H, Takahashi M. Mycoplasma Ocular Infection in Subretinal Graft Transplantation of iPS Cells-Derived Retinal Pigment Epithelial Cells. Invest Ophthalmol Vis Sci. 2019;60:1298-1308 pubmed publisher
Su S, Zhao J, Xing Y, Zhang X, Liu J, Ouyang Q, et al. Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages. Cell. 2018;175:442-457.e23 pubmed publisher
Fujii S, Yoshida S, Inagaki E, Hatou S, Tsubota K, Takahashi M, et al. Immunological properties of neural crest cells derived from human induced pluripotent stem cells. Stem Cells Dev. 2018;: pubmed publisher
Taylor H, Calantone N, D Aquila R. mTOR signaling mediates effects of common gamma-chain cytokines on T cell proliferation and exhaustion: implications for HIV-1 persistence and cure research. AIDS. 2018;32:2847-2851 pubmed publisher
Trotta E, Bessette P, Silveria S, Ely L, Jude K, Le D, et al. A human anti-IL-2 antibody that potentiates regulatory T cells by a structure-based mechanism. Nat Med. 2018;24:1005-1014 pubmed publisher
Gao Y, Liu Z, Ding Z, Hou S, Li J, Jiang K. MicroRNA-155 increases colon cancer chemoresistance to cisplatin by targeting forkhead box O3. Oncol Lett. 2018;15:4781-4788 pubmed publisher
Day C, Abrahams D, Harris L, van Rooyen M, Stone L, de Kock M, et al. HIV-1 Infection Is Associated with Depletion and Functional Impairment of Mycobacterium tuberculosis-Specific CD4 T Cells in Individuals with Latent Tuberculosis Infection. J Immunol. 2017;199:2069-2080 pubmed publisher
Timilshina M, Kang Y, Dahal I, You Z, Nam T, Kim K, et al. BJ-3105, a 6-Alkoxypyridin-3-ol Analog, Impairs T Cell Differentiation and Prevents Experimental Autoimmune Encephalomyelitis Disease Progression. PLoS ONE. 2017;12:e0168942 pubmed publisher
Wei C, Mei J, Tang L, Liu Y, Li D, Li M, et al. 1-Methyl-tryptophan attenuates regulatory T cells differentiation due to the inhibition of estrogen-IDO1-MRC2 axis in endometriosis. Cell Death Dis. 2016;7:e2489 pubmed publisher
Sugita S, Iwasaki Y, Makabe K, Kimura T, Futagami T, Suegami S, et al. Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors. Stem Cell Reports. 2016;7:619-634 pubmed publisher
Jung M, Ören B, Mora J, Mertens C, Dziumbla S, Popp R, et al. Lipocalin 2 from macrophages stimulated by tumor cell-derived sphingosine 1-phosphate promotes lymphangiogenesis and tumor metastasis. Sci Signal. 2016;9:ra64 pubmed publisher
Liu B, Ma Y, Zhang Y, Zhang C, Yi J, Zhuang R, et al. CD8low CD100- T Cells Identify a Novel CD8 T Cell Subset Associated with Viral Control during Human Hantaan Virus Infection. J Virol. 2015;89:11834-44 pubmed publisher
Pembroke T, Christian A, Jones E, Hills R, Wang E, Gallimore A, et al. The paradox of NKp46+ natural killer cells: drivers of severe hepatitis C virus-induced pathology but in-vivo resistance to interferon ? treatment. Gut. 2014;63:515-24 pubmed publisher
Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed publisher
Muellenbeck M, Ueberheide B, Amulic B, Epp A, Fenyo D, Busse C, et al. Atypical and classical memory B cells produce Plasmodium falciparum neutralizing antibodies. J Exp Med. 2013;210:389-99 pubmed publisher
Perreau M, Savoye A, De Crignis E, Corpataux J, Cubas R, Haddad E, et al. Follicular helper T cells serve as the major CD4 T cell compartment for HIV-1 infection, replication, and production. J Exp Med. 2013;210:143-56 pubmed publisher
product information
Antigen :
Ki-67
Apps. Abbrev. :
ICFC
Cat # :
350504
Clone :
Ki-67
Item :
PE anti-human Ki-67
Isotype :
Mouse IgG1, κ
Other Names :
Antigen Ki-67
Size :
100 tests
Price (USD) :
347 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE
Immunogen :
Nuclei of the Hodgkin lymphoma cell line L428
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1, Western blotting^3, and immunofluorescence microscopy^4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10^6/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA