product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human Ki-67
catalog :
350501
quantity :
25 μg
price :
65 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Ki-67
reactivity :
human, mouse
application :
western blot, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 30
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:200; loading ...; fig s2c
Oberhardt V, Luxenburger H, Kemming J, Schulien I, Ciminski K, Giese S, et al. Rapid and stable mobilization of CD8+ T cells by SARS-CoV-2 mRNA vaccine. Nature. 2021;597:268-273 pubmed publisher
  • flow cytometry; human; 3:50; loading ...; fig 3b
Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
  • flow cytometry; human; 1:20; loading ...; fig 3a
Huang Y, Huang J, Huang Y, Gan L, Long L, Pu A, et al. TFRC promotes epithelial ovarian cancer cell proliferation and metastasis via up-regulation of AXIN2 expression. Am J Cancer Res. 2020;10:131-147 pubmed
  • flow cytometry; human; loading ...; fig 1b
Remmerswaal E, Hombrink P, Nota B, Pircher H, ten Berge I, van Lier R, et al. Expression of IL-7Rα and KLRG1 defines functionally distinct CD8+ T-cell populations in humans. Eur J Immunol. 2019;49:694-708 pubmed publisher
  • flow cytometry; human; loading ...; fig s6a
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Li M, Zhang W, Liu J, Li M, Zhang Y, Xiong Y, et al. Dynamic changes in the immunological characteristics of T lymphocytes in surviving patients with severe fever with thrombocytopenia syndrome (SFTS). Int J Infect Dis. 2018;70:72-80 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Amodio D, Cotugno N, Macchiarulo G, Rocca S, Dimopoulos Y, Castrucci M, et al. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination. J Immunol. 2018;200:538-550 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Vanegas N, Vernot J. Loss of quiescence and self-renewal capacity of hematopoietic stem cell in an in vitro leukemic niche. Exp Hematol Oncol. 2017;6:2 pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
Ashizawa T, Iizuka A, Nonomura C, Kondou R, Maeda C, Miyata H, et al. Antitumor Effect of Programmed Death-1 (PD-1) Blockade in Humanized the NOG-MHC Double Knockout Mouse. Clin Cancer Res. 2017;23:149-158 pubmed publisher
  • flow cytometry; human; fig s2
Leeansyah E, Svärd J, Dias J, Buggert M, Nyström J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed publisher
  • flow cytometry; mouse; fig 4
Sakamoto H, Takeda N, Arai F, Hosokawa K, García P, Suda T, et al. Determining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Stem Cells. 2015;33:479-90 pubmed publisher
Qin X, Furutani Y, Yonezawa K, Shimizu N, Kato Murayama M, Shirouzu M, et al. Targeting transglutaminase 2 mediated exostosin glycosyltransferase 1 signaling in liver cancer stem cells with acyclic retinoid. Cell Death Dis. 2023;14:358 pubmed publisher
Ferreira J, Granja S, Almeida A, Baltazar F, Gon xe7 alves M, Preto A, et al. Targeting Lysosomes in Colorectal Cancer: Exploring the Anticancer Activity of a New Benzo[a]phenoxazine Derivative. Int J Mol Sci. 2022;24: pubmed publisher
Junghof J, Kogure Y, Yu T, Verdugo Sivianes E, Narita M, Lucena Cacace A, et al. CDH18 is a fetal epicardial biomarker regulating differentiation towards vascular smooth muscle cells. NPJ Regen Med. 2022;7:14 pubmed publisher
Wang L, Tang Y, Wu H, Shan G. TCF12 activates MAGT1 expression to regulate the malignant progression of pancreatic carcinoma cells. Oncol Lett. 2022;23:62 pubmed publisher
Tailor D, Resendez A, Garcia Marques F, Pandrala M, Going C, Bermudez A, et al. Y box binding protein 1 inhibition as a targeted therapy for ovarian cancer. Cell Chem Biol. 2021;: pubmed publisher
Xie S, Shan X, Yau V, Zhang J, Zhang X, Yan Y, et al. Hyperion imaging system reveals heterogeneous tumor microenvironment of oral squamous cell carcinoma patients at T1N0M0 stage. Ann Transl Med. 2020;8:1513 pubmed publisher
Mann E, Menon M, Knight S, Konkel J, Jagger C, Shaw T, et al. Longitudinal immune profiling reveals key myeloid signatures associated with COVID-19. Sci Immunol. 2020;5: pubmed publisher
Eccles J, Turner R, Kirk N, Muehling L, Borish L, Steinke J, et al. T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection. Cell Rep. 2020;30:351-366.e7 pubmed publisher
O Boyle K, Ohtani T, Manne S, Bengsch B, Henrickson S, Wherry E, et al. Exploration of T-Cell Diversity Using Mass Cytometry. Methods Mol Biol. 2020;2111:1-20 pubmed publisher
Wang Z, Zhou L, Xiong Y, Yu S, Li H, Fan J, et al. Salinomycin exerts anti-colorectal cancer activity by targeting the β-catenin/T-cell factor complex. Br J Pharmacol. 2019;176:3390-3406 pubmed publisher
Xiong Y, Zhou L, Su Z, Song J, Sun Q, Liu S, et al. Longdaysin inhibits Wnt/β-catenin signaling and exhibits antitumor activity against breast cancer. Onco Targets Ther. 2019;12:993-1005 pubmed publisher
Saraiva D, Jacinto A, Borralho P, Braga S, Cabral M. HLA-DR in Cytotoxic T Lymphocytes Predicts Breast Cancer Patients' Response to Neoadjuvant Chemotherapy. Front Immunol. 2018;9:2605 pubmed publisher
Agrawal N, Streaţă I, Pei G, Weiner J, Kotze L, Bandermann S, et al. Human Monocytic Suppressive Cells Promote Replication of Mycobacterium tuberculosis and Alter Stability of in vitro Generated Granulomas. Front Immunol. 2018;9:2417 pubmed publisher
Vining K, Scherba J, Bever A, Alexander M, Celiz A, Mooney D. Synthetic Light-Curable Polymeric Materials Provide a Supportive Niche for Dental Pulp Stem Cells. Adv Mater. 2017;: pubmed publisher
Santos R, Bai L, Singh P, Murakami N, Fan H, Zhan W, et al. Structure of human immunoproteasome with a reversible and noncompetitive inhibitor that selectively inhibits activated lymphocytes. Nat Commun. 2017;8:1692 pubmed publisher
Bruno T, Ebner P, Moore B, Squalls O, Waugh K, Eruslanov E, et al. Antigen-Presenting Intratumoral B Cells Affect CD4+ TIL Phenotypes in Non-Small Cell Lung Cancer Patients. Cancer Immunol Res. 2017;5:898-907 pubmed publisher
Zhou H, Mak P, Mu H, Mak D, Zeng Z, Cortes J, et al. Combined inhibition of ?-catenin and Bcr-Abl synergistically targets tyrosine kinase inhibitor-resistant blast crisis chronic myeloid leukemia blasts and progenitors in vitro and in vivo. Leukemia. 2017;31:2065-2074 pubmed publisher
Guha P, Kaptan E, Bandyopadhyaya G, Kaczanowska S, Davila E, Thompson K, et al. Cod glycopeptide with picomolar affinity to galectin-3 suppresses T-cell apoptosis and prostate cancer metastasis. Proc Natl Acad Sci U S A. 2013;110:5052-7 pubmed publisher
product information
Antigen :
Ki-67
Apps. Abbrev. :
ICFC, CyTOF®, ICC, WB, IHC-F
Cat # :
350501
Clone :
Ki-67
Item :
Purified anti-human Ki-67
Isotype :
Mouse IgG1, κ
Other Names :
Antigen Ki-67
Size :
25 μg
Price (USD) :
65 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Nuclei of the Hodgkin lymphoma cell line L428
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1, Western blotting^3, and immunofluorescence microscopy^4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 10^6/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA