product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD8 (Maxpar® Ready)
catalog :
344727
quantity :
100 μg
price :
171 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SK1
reactivity :
human, mouse, rhesus macaque
application :
immunocytochemistry, flow cytometry, mass cytometry
more info or order :
citations: 36
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1a
Yue X, Petersen F, Shu Y, Kasper B, Magatsin J, Ahmadi M, et al. Transfer of PBMC From SSc Patients Induces Autoantibodies and Systemic Inflammation in Rag2-/-/IL2rg-/- Mice. Front Immunol. 2021;12:677970 pubmed publisher
  • flow cytometry; human; loading ...; fig 4
Martínez Zamudio R, Dewald H, Vasilopoulos T, Gittens Williams L, Fitzgerald Bocarsly P, Herbig U. Senescence-associated β-galactosidase reveals the abundance of senescent CD8+ T cells in aging humans. Aging Cell. 2021;20:e13344 pubmed publisher
  • flow cytometry; human; 3:50; loading ...; fig 3a
Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...
Hood S, Cosma G, Foulds G, Johnson C, Reeder S, McArdle S, et al. Identifying prostate cancer and its clinical risk in asymptomatic men using machine learning of high dimensional peripheral blood flow cytometric natural killer cell subset phenotyping data. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig s3a
Fischer M, Ruhnau J, Schulze J, Obst D, Floel A, Vogelgesang A. Spermine and spermidine modulate T-cell function in older adults with and without cognitive decline ex vivo. Aging (Albany NY). 2020;12:13716-13739 pubmed publisher
  • flow cytometry; human; 1:400; loading ...; fig s1i
Cao W, Fang F, Gould T, Li X, Kim C, Gustafson C, et al. Ecto-NTPDase CD39 is a negative checkpoint that inhibits follicular helper cell generation. J Clin Invest. 2020;130:3422-3436 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a, 1b
Ma T, Luo X, George A, Mukherjee G, Sen N, Spitzer T, et al. HIV efficiently infects T cells from the endometrium and remodels them to promote systemic viral spread. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Callender L, Carroll E, Bober E, Akbar A, Solito E, Henson S. Mitochondrial mass governs the extent of human T cell senescence. Aging Cell. 2020;19:e13067 pubmed publisher
  • flow cytometry; human; 0.5 ug/ml; loading ...; fig 1d
Le Nours J, Gherardin N, Ramarathinam S, Awad W, Wiede F, Gully B, et al. A class of γδ T cell receptors recognize the underside of the antigen-presenting molecule MR1. Science. 2019;366:1522-1527 pubmed publisher
  • flow cytometry; human; loading ...; fig e1a, 3h
Lynn R, Weber E, Sotillo E, Gennert D, Xu P, Good Z, et al. c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature. 2019;576:293-300 pubmed publisher
  • flow cytometry; human; loading ...; fig 3d
Strickley J, Messerschmidt J, Awad M, Li T, Hasegawa T, Ha D, et al. Immunity to commensal papillomaviruses protects against skin cancer. Nature. 2019;: pubmed publisher
  • mass cytometry; human; loading ...; fig s1
Fernandez I, Baxter R, Garcia Perez J, Vendrame E, Ranganath T, Kong D, et al. A novel human IL2RB mutation results in T and NK cell-driven immune dysregulation. J Exp Med. 2019;216:1255-1267 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Wen Z, Jin K, Shen Y, Yang Z, Li Y, Wu B, et al. N-myristoyltransferase deficiency impairs activation of kinase AMPK and promotes synovial tissue inflammation. Nat Immunol. 2019;20:313-325 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig s1b
Otsuka Y, Watanabe E, Shinya E, Okura S, Saeki H, Geijtenbeek T, et al. Differentiation of Langerhans Cells from Monocytes and Their Specific Function in Inducing IL-22-Specific Th Cells. J Immunol. 2018;201:3006-3016 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Moysi E, Pallikkuth S, de Armas L, Gonzalez L, Ambrozak D, George V, et al. Altered immune cell follicular dynamics in HIV infection following influenza vaccination. J Clin Invest. 2018;128:3171-3185 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Trapecar M, Khan S, Cohn B, Wu F, Sanjabi S. B cells are the predominant mediators of early systemic viral dissemination during rectal LCMV infection. Mucosal Immunol. 2018;11:1158-1167 pubmed publisher
  • flow cytometry; human; loading ...; fig 1c
Pizzolla A, Nguyen T, Sant S, Jaffar J, Loudovaris T, Mannering S, et al. Influenza-specific lung-resident memory T cells are proliferative and polyfunctional and maintain diverse TCR profiles. J Clin Invest. 2018;128:721-733 pubmed publisher
  • flow cytometry; human; loading ...; fig 2c
Moreno Cubero E, Subira D, Sanz de Villalobos E, Parra Cid T, Madejon A, Miquel J, et al. According to Hepatitis C Virus (HCV) Infection Stage, Interleukin-7 Plus 4-1BB Triggering Alone or Combined with PD-1 Blockade Increases TRAF1low HCV-Specific CD8+ Cell Reactivity. J Virol. 2018;92: pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Meng W, Tang A, Ye X, Gui X, Li L, Fan X, et al. Targeting Human-Cytomegalovirus-Infected Cells by Redirecting T Cells Using an Anti-CD3/Anti-Glycoprotein B Bispecific Antibody. Antimicrob Agents Chemother. 2018;62: pubmed publisher
  • flow cytometry; human; loading ...; fig 4b
Matos T, O Malley J, Lowry E, Hamm D, Kirsch I, Robins H, et al. Clinically resolved psoriatic lesions contain psoriasis-specific IL-17-producing ?? T cell clones. J Clin Invest. 2017;127:4031-4041 pubmed publisher
  • flow cytometry; mouse; fig s3b
Tripathi D, Venkatasubramanian S, Cheekatla S, Paidipally P, Welch E, Tvinnereim A, et al. A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice. Nat Commun. 2016;7:13896 pubmed publisher
  • flow cytometry; human; 1:50; loading ...; fig 2a
Siegers G, Barreira C, Postovit L, Dekaban G. CD11d ?2 integrin expression on human NK, B, and ?? T cells. J Leukoc Biol. 2017;101:1029-1035 pubmed publisher
  • flow cytometry; human; loading ...; fig s1c
Galindo Albarrán A, López Portales O, Gutiérrez Reyna D, Rodríguez Jorge O, Sánchez Villanueva J, Ramirez Pliego O, et al. CD8+ T Cells from Human Neonates Are Biased toward an Innate Immune Response. Cell Rep. 2016;17:2151-2160 pubmed publisher
  • flow cytometry; human; loading ...; tbl 1
Sumatoh H, Teng K, Cheng Y, Newell E. Optimization of mass cytometry sample cryopreservation after staining. Cytometry A. 2017;91:48-61 pubmed publisher
  • flow cytometry; human; loading ...; fig 6e
Landtwing V, Raykova A, Pezzino G, Beziat V, Marcenaro E, Graf C, et al. Cognate HLA absence in trans diminishes human NK cell education. J Clin Invest. 2016;126:3772-3782 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig s2
Javed A, Leuchte N, Neumann B, Sopper S, Sauermann U. Noncytolytic CD8+ Cell Mediated Antiviral Response Represents a Strong Element in the Immune Response of Simian Immunodeficiency Virus-Infected Long-Term Non-Progressing Rhesus Macaques. PLoS ONE. 2015;10:e0142086 pubmed publisher
  • flow cytometry; human; tbl s1
Weinberg A, Muresan P, Richardson K, Fenton T, Domínguez T, Bloom A, et al. Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine. PLoS ONE. 2015;10:e0122431 pubmed publisher
  • flow cytometry; human; fig 2
Bradley S, Chen Z, Melendez B, Talukder A, Khalili J, Rodríguez Cruz T, et al. BRAFV600E Co-opts a Conserved MHC Class I Internalization Pathway to Diminish Antigen Presentation and CD8+ T-cell Recognition of Melanoma. Cancer Immunol Res. 2015;3:602-9 pubmed publisher
  • immunocytochemistry; human
Galindo Albarrán A, Ramirez Pliego O, Labastida Conde R, Melchy Pérez E, Liquitaya Montiel A, Esquivel Guadarrama F, et al. CD43 signals prepare human T cells to receive cytokine differentiation signals. J Cell Physiol. 2014;229:172-80 pubmed
  • flow cytometry; human; loading ...; fig 2
Svajger U, Obermajer N, Jeras M. IFN-?-rich environment programs dendritic cells toward silencing of cytotoxic immune responses. J Leukoc Biol. 2014;95:33-46 pubmed publisher
Stensland Z, Coleman B, Rihanek M, Baxter R, Gottlieb P, Hsieh E, et al. Peripheral immunophenotyping of AITD subjects reveals alterations in immune cells in pediatric vs adult-onset AITD. iScience. 2022;25:103626 pubmed publisher
Mishra A, Lai G, Yao L, Aung T, Shental N, Rotter Maskowitz A, et al. Microbial exposure during early human development primes fetal immune cells. Cell. 2021;184:3394-3409.e20 pubmed publisher
Jordan S, Tung N, Casanova Acebes M, Chang C, Cantoni C, Zhang D, et al. Dietary Intake Regulates the Circulating Inflammatory Monocyte Pool. Cell. 2019;178:1102-1114.e17 pubmed publisher
Cheung P, Vallania F, Warsinske H, Donato M, Schaffert S, Chang S, et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. 2018;173:1385-1397.e14 pubmed publisher
Ch en I, Tsau J, Molkentin J, Komatsu M, Hedrick S. Mechanisms of necroptosis in T cells. J Exp Med. 2011;208:633-41 pubmed publisher
product information
Antigen :
CD8
Apps. Abbrev. :
Flow Cytometry, CyTOF®
Cat # :
344727
Clone :
SK1
Item :
Purified anti-human CD8 (Maxpar® Ready)
Isotype :
Mouse IgG1, κ
Other Names :
T8, Leu2
Size :
100 μg
Price (USD) :
171 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PUREMAX
Application Notes :
Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics 8 , immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX) 9,10 . This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA