product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-human CD4
catalog :
344635
quantity :
25 tests
price :
124 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF647
clone name :
SK3
reactivity :
human, mouse
application :
flow cytometry, blocking or activating experiments, mass cytometry
more info or order :
citations: 23
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 2b
Jin Y, Lorvik K, Jin Y, Beck C, Sike A, Persiconi I, et al. Development of STEAP1 targeting chimeric antigen receptor for adoptive cell therapy against cancer. Mol Ther Oncolytics. 2022;26:189-206 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b, 3a
Laffey K, Stiles R, Ludescher M, Davis T, Khwaja S, Bram R, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022;119:e2118529119 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Pan C, Wu Q, Wang S, Mei Z, Zhang L, Gao X, et al. Combination with Toll-like receptor 4 (TLR4) agonist reverses GITR agonism mediated M2 polarization of macrophage in Hepatocellular carcinoma. Oncoimmunology. 2022;11:2073010 pubmed publisher
  • flow cytometry; human; 2 ug/ml; loading ...; fig 1d
Le Nours J, Gherardin N, Ramarathinam S, Awad W, Wiede F, Gully B, et al. A class of γδ T cell receptors recognize the underside of the antigen-presenting molecule MR1. Science. 2019;366:1522-1527 pubmed publisher
  • mass cytometry; human; loading ...; fig s1
Fernandez I, Baxter R, Garcia Perez J, Vendrame E, Ranganath T, Kong D, et al. A novel human IL2RB mutation results in T and NK cell-driven immune dysregulation. J Exp Med. 2019;216:1255-1267 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Koppejan H, Jansen D, Hameetman M, Thomas R, Toes R, van Gaalen F. Altered composition and phenotype of mucosal-associated invariant T cells in early untreated rheumatoid arthritis. Arthritis Res Ther. 2019;21:3 pubmed publisher
  • mass cytometry; human; loading ...; fig 2a
Chew V, Lai L, Pan L, Lim C, Li J, Ong R, et al. Delineation of an immunosuppressive gradient in hepatocellular carcinoma using high-dimensional proteomic and transcriptomic analyses. Proc Natl Acad Sci U S A. 2017;114:E5900-E5909 pubmed publisher
  • flow cytometry; human; loading ...; tbl 1
van der Velden V, Flores Montero J, Perez Andres M, Martin Ayuso M, Crespo O, Blanco E, et al. Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples. J Immunol Methods. 2017;: pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Van Puyenbroeck V, Claeys E, Schols D, Bell T, Vermeire K. A Proteomic Survey Indicates Sortilin as a Secondary Substrate of the ER Translocation Inhibitor Cyclotriazadisulfonamide (CADA). Mol Cell Proteomics. 2017;16:157-167 pubmed publisher
  • flow cytometry; human; loading ...; tbl 1
Sumatoh H, Teng K, Cheng Y, Newell E. Optimization of mass cytometry sample cryopreservation after staining. Cytometry A. 2017;91:48-61 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Daud A, Loo K, Pauli M, Sanchez Rodriguez R, Sandoval P, Taravati K, et al. Tumor immune profiling predicts response to anti-PD-1 therapy in human melanoma. J Clin Invest. 2016;126:3447-52 pubmed publisher
  • flow cytometry; human; fig s2a
Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed publisher
  • flow cytometry; human; fig 1
Rissiek A, Baumann I, Cuapio A, Mautner A, Kolster M, Arck P, et al. The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation. J Autoimmun. 2015;58:12-20 pubmed publisher
  • blocking or activating experiments; human
Chattergoon M, Latanich R, Quinn J, Winter M, Buckheit R, Blankson J, et al. HIV and HCV activate the inflammasome in monocytes and macrophages via endosomal Toll-like receptors without induction of type 1 interferon. PLoS Pathog. 2014;10:e1004082 pubmed publisher
Bhowmick D, van Diepen F, Pfauth A, Tissier R, Ratliff M. A gain and dynamic range independent index to quantify spillover spread to aid panel design in flow cytometry. Sci Rep. 2021;11:20553 pubmed publisher
Xu S, Chaudhary O, Rodríguez Morales P, Sun X, Chen D, Zappasodi R, et al. Uptake of oxidized lipids by the scavenger receptor CD36 promotes lipid peroxidation and dysfunction in CD8+ T cells in tumors. Immunity. 2021;54:1561-1577.e7 pubmed publisher
Liu Z, Gu Y, Shin A, Zhang S, Ginhoux F. Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry. STAR Protoc. 2020;1:100029 pubmed publisher
Pfirschke C, Engblom C, Gungabeesoon J, Lin Y, Rickelt S, Zilionis R, et al. Tumor-Promoting Ly-6G+ SiglecFhigh Cells Are Mature and Long-Lived Neutrophils. Cell Rep. 2020;32:108164 pubmed publisher
Baker G, Muhlich J, Palaniappan S, Moore J, Davis S, Santagata S, et al. SYLARAS: A Platform for the Statistical Analysis and Visual Display of Systemic Immunoprofiling Data and Its Application to Glioblastoma. Cell Syst. 2020;11:272-285.e9 pubmed publisher
Voeller J, Erbe A, Slowinski J, Rasmussen K, Carlson P, Hoefges A, et al. Combined innate and adaptive immunotherapy overcomes resistance of immunologically cold syngeneic murine neuroblastoma to checkpoint inhibition. J Immunother Cancer. 2019;7:344 pubmed publisher
Liu Z, Gu Y, Chakarov S, Blériot C, Kwok I, Chen X, et al. Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells. Cell. 2019;178:1509-1525.e19 pubmed publisher
Yu Y, O Koren E, Hotten D, Kan M, Kopin D, Nelson E, et al. A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflamed Murine Non-Lymphoid Tissues. PLoS ONE. 2016;11:e0150606 pubmed publisher
Wang L, Abbasi F, Ornatsky O, Cole K, Misakian M, Gaigalas A, et al. Human CD4+ lymphocytes for antigen quantification: characterization using conventional flow cytometry and mass cytometry. Cytometry A. 2012;81:567-75 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
FC
Cat # :
344635
Clone :
SK3
Item :
Alexa Fluor® 647 anti-human CD4
Isotype :
Mouse IgG1, κ
Other Names :
T4, Leu3a
Size :
25 tests
Price (USD) :
124 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A647
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA