product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD4
catalog :
344602
quantity :
100 μg
price :
42 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SK3
reactivity :
human, mouse
application :
immunoprecipitation, flow cytometry, blocking or activating experiments, mass cytometry
more info or order :
citations: 27
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 2b
Jin Y, Lorvik K, Jin Y, Beck C, Sike A, Persiconi I, et al. Development of STEAP1 targeting chimeric antigen receptor for adoptive cell therapy against cancer. Mol Ther Oncolytics. 2022;26:189-206 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b, 3a
Laffey K, Stiles R, Ludescher M, Davis T, Khwaja S, Bram R, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022;119:e2118529119 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Pan C, Wu Q, Wang S, Mei Z, Zhang L, Gao X, et al. Combination with Toll-like receptor 4 (TLR4) agonist reverses GITR agonism mediated M2 polarization of macrophage in Hepatocellular carcinoma. Oncoimmunology. 2022;11:2073010 pubmed publisher
  • flow cytometry; human; 2 ug/ml; loading ...; fig 1d
Le Nours J, Gherardin N, Ramarathinam S, Awad W, Wiede F, Gully B, et al. A class of γδ T cell receptors recognize the underside of the antigen-presenting molecule MR1. Science. 2019;366:1522-1527 pubmed publisher
  • mass cytometry; human; loading ...; fig s1
Fernandez I, Baxter R, Garcia Perez J, Vendrame E, Ranganath T, Kong D, et al. A novel human IL2RB mutation results in T and NK cell-driven immune dysregulation. J Exp Med. 2019;216:1255-1267 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Koppejan H, Jansen D, Hameetman M, Thomas R, Toes R, van Gaalen F. Altered composition and phenotype of mucosal-associated invariant T cells in early untreated rheumatoid arthritis. Arthritis Res Ther. 2019;21:3 pubmed publisher
  • flow cytometry; human; loading ...; tbl 1
van der Velden V, Flores Montero J, Perez Andres M, Martin Ayuso M, Crespo O, Blanco E, et al. Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples. J Immunol Methods. 2017;: pubmed publisher
  • flow cytometry; human; loading ...; tbl 1
Sumatoh H, Teng K, Cheng Y, Newell E. Optimization of mass cytometry sample cryopreservation after staining. Cytometry A. 2017;91:48-61 pubmed publisher
  • flow cytometry; human; fig s2a
Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed publisher
  • flow cytometry; human; fig 1
Rissiek A, Baumann I, Cuapio A, Mautner A, Kolster M, Arck P, et al. The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation. J Autoimmun. 2015;58:12-20 pubmed publisher
  • blocking or activating experiments; human
Chattergoon M, Latanich R, Quinn J, Winter M, Buckheit R, Blankson J, et al. HIV and HCV activate the inflammasome in monocytes and macrophages via endosomal Toll-like receptors without induction of type 1 interferon. PLoS Pathog. 2014;10:e1004082 pubmed publisher
Mascarau R, Woottum M, Fromont L, Gence R, Cantaloube Ferrieu V, Vahlas Z, et al. Productive HIV-1 infection of tissue macrophages by fusion with infected CD4+ T cells. J Cell Biol. 2023;222: pubmed publisher
Paulikat A, T xf6 lken L, Jachmann L, Burchhardt G, Hammerschmidt S, Siemens N. Streptococcus pneumoniae Impairs Maturation of Human Dendritic Cells and Consequent Activation of CD4+ T Cells via Pneumolysin. J Innate Immun. 2022;14:569-580 pubmed publisher
Albanese M, Ruhle A, Mittermaier J, Mejías Pérez E, Gapp M, Linder A, et al. Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4+ T cells allows complex functional analyses. Nat Methods. 2022;19:81-89 pubmed publisher
Tolbert W, Nguyen D, Tuyishime M, Crowley A, Chen Y, Jha S, et al. Structure and Fc-Effector Function of Rhesusized Variants of Human Anti-HIV-1 IgG1s. Front Immunol. 2021;12:787603 pubmed publisher
Song S, Manook M, Kwun J, Jackson A, Knechtle S, Kelsoe G. A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines. Commun Biol. 2021;4:1338 pubmed publisher
Rouers A, Chng M, Lee B, Rajapakse M, Kaur K, Toh Y, et al. Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection. Cell Rep Med. 2021;2:100278 pubmed publisher
Draganov D, Han Z, Rana A, Bennett N, Irvine D, Lee P. Ivermectin converts cold tumors hot and synergizes with immune checkpoint blockade for treatment of breast cancer. NPJ Breast Cancer. 2021;7:22 pubmed publisher
Mann E, Menon M, Knight S, Konkel J, Jagger C, Shaw T, et al. Longitudinal immune profiling reveals key myeloid signatures associated with COVID-19. Sci Immunol. 2020;5: pubmed publisher
Hsiao F, Frouard J, Gramatica A, Xie G, Telwatte S, Lee G, et al. Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection. PLoS Pathog. 2020;16:e1008450 pubmed publisher
Chng M, Lim M, Rouers A, Becht E, Lee B, Macary P, et al. Large-Scale HLA Tetramer Tracking of T Cells during Dengue Infection Reveals Broad Acute Activation and Differentiation into Two Memory Cell Fates. Immunity. 2019;51:1119-1135.e5 pubmed publisher
Del Alcazar D, Wang Y, He C, Wendel B, Del Rio Estrada P, Lin J, et al. Mapping the Lineage Relationship between CXCR5+ and CXCR5- CD4+ T Cells in HIV-Infected Human Lymph Nodes. Cell Rep. 2019;28:3047-3060.e7 pubmed publisher
Ramaglia V, Sheikh Mohamed S, Legg K, Park C, Rojas O, Zandee S, et al. Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry. elife. 2019;8: pubmed publisher
Hyun J, McMahon R, Lang A, Edwards J, Badilla A, Greene M, et al. HIV and HCV augments inflammatory responses through increased TREM-1 expression and signaling in Kupffer and Myeloid cells. PLoS Pathog. 2019;15:e1007883 pubmed publisher
Ferdosi S, Ewaisha R, Moghadam F, Krishna S, Park J, Ebrahimkhani M, et al. Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes. Nat Commun. 2019;10:1842 pubmed publisher
Toyoda M, Ogata Y, Mahiti M, Maeda Y, Kuang X, Miura T, et al. Differential Ability of Primary HIV-1 Nef Isolates To Downregulate HIV-1 Entry Receptors. J Virol. 2015;89:9639-52 pubmed publisher
Wang L, Abbasi F, Ornatsky O, Cole K, Misakian M, Gaigalas A, et al. Human CD4+ lymphocytes for antigen quantification: characterization using conventional flow cytometry and mass cytometry. Cytometry A. 2012;81:567-75 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
FC, IP
Cat # :
344602
Clone :
SK3
Item :
Purified anti-human CD4
Isotype :
Mouse IgG1, κ
Other Names :
T4, Leu3a
Size :
100 μg
Price (USD) :
42 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA