Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Human Th17 Flow™ Kit

339401

25 tests

586 USD

ICFC

339401

BL168; UCHT1; RPA-T4

Human Th17 Flow™ Kit

Mouse IgG1, κ (all clones)

Th17 kit, Th-17 kit, Th 17 kit

25 tests

586 USD

Human

Mouse

SET

Staining Procedure:1. Distribute 0.5-1 X 10^6 cells/100 µl/tube into 12 X 75 mm plastic testing tubes.2. Add 0.5 ml/tube Fixation Buffer (Cat. No. 420801) to each tube, votex and incubate at room temperature for 20 minutes.3. Centrifuge at 350 x g for 5 minutes, discard supernatant.4. Prepare 1X Intracellular Staining Perm Wash Buffer (Cat. No. 421002) by diluting 1 part of 10X Intracellular Staining Perm Wash Buffer (Cat. No. 421002) with 9 part of DI water.5. Add 2ml 1X Intracellular Staining Perm Wash Buffer to each tube, vortex and incubate at room temperature for 20 minutes, then spin at 350 x g for 5 minutes and remove the supernatant.6. Wash once by adding 2ml 1X Intracellular Staining Perm Wash Buffer to each tube, spin and remove the supernatant.7. Resuspend the pellet in 100 µl of 1X Intracellular Staining Perm Wash Buffer.8. Add 20 µl Alexa Fluor® 647 mouse IgG1, k isotype control/CD3 FITC/CD4 PE or IL-17 Alexa Fluor® 647/CD3 FITC/CD4 PE into appropriate tube and incubate at room temperature in the dark for 30 minutes.10. Wash twice with 2ml 1X Intracellular Staining Perm Wash Buffer, and resuspend in 0.5ml cell staining buffer then analyze under flow cytometer with appropriate instrument setting.Note:1. The Fixation buffer contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.2. Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.3. Please note that single color controls are required to perform instrument compensation and are not included in the kit.;