product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD40
catalog :
334302
quantity :
100 μg
price :
102 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5C3
reactivity :
human, rhesus macaque
application :
immunocytochemistry, flow cytometry, blocking or activating experiments, mass cytometry
more info or order :
citations: 21
Published Application/Species/Sample/DilutionReference
  • mass cytometry; human; 500 ug/ml; loading ...; fig s11a
Helmink B, Reddy S, Gao J, Zhang S, Basar R, Thakur R, et al. B cells and tertiary lymphoid structures promote immunotherapy response. Nature. 2020;577:549-555 pubmed publisher
  • flow cytometry; human; loading ...; fig 6a
Salio M, Gasser O, González López C, Martens A, Veerapen N, Gileadi U, et al. Activation of Human Mucosal-Associated Invariant T Cells Induces CD40L-Dependent Maturation of Monocyte-Derived and Primary Dendritic Cells. J Immunol. 2017;199:2631-2638 pubmed publisher
  • flow cytometry; human; fig 3e
Papa I, Saliba D, Ponzoni M, Bustamante S, Canete P, Gonzalez Figueroa P, et al. TFH-derived dopamine accelerates productive synapses in germinal centres. Nature. 2017;547:318-323 pubmed publisher
  • flow cytometry; human; loading ...; fig s6a
Pryke K, Abraham J, Sali T, Gall B, Archer I, Liu A, et al. A Novel Agonist of the TRIF Pathway Induces a Cellular State Refractory to Replication of Zika, Chikungunya, and Dengue Viruses. MBio. 2017;8: pubmed publisher
  • flow cytometry; human; 1:50; loading ...; fig s1d
Smith N, Pietrancosta N, Davidson S, Dutrieux J, Chauveau L, Cutolo P, et al. Natural amines inhibit activation of human plasmacytoid dendritic cells through CXCR4 engagement. Nat Commun. 2017;8:14253 pubmed publisher
  • flow cytometry; human; fig 3e
Martin Gayo E, Cronin J, Hickman T, Ouyang Z, Lindqvist M, Kolb K, et al. Circulating CXCR5+CXCR3+PD-1lo Tfh-like cells in HIV-1 controllers with neutralizing antibody breadth. JCI Insight. 2017;2:e89574 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
  • immunocytochemistry; human; fig 1c
Lee G, Fujita M, Takaoka K, Murakami Y, Fujihara Y, Kanzawa N, et al. A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO. J Cell Biol. 2016;215:705-718 pubmed
  • flow cytometry; human; loading ...
Tagawa T, Albanese M, Bouvet M, Moosmann A, Mautner J, Heissmeyer V, et al. Epstein-Barr viral miRNAs inhibit antiviral CD4+ T cell responses targeting IL-12 and peptide processing. J Exp Med. 2016;213:2065-80 pubmed publisher
  • flow cytometry; human; 1:200; loading ...; fig 7i
Göbel K, Pankratz S, Asaridou C, Herrmann A, Bittner S, Merker M, et al. Blood coagulation factor XII drives adaptive immunity during neuroinflammation via CD87-mediated modulation of dendritic cells. Nat Commun. 2016;7:11626 pubmed publisher
  • flow cytometry; human; loading ...; tbl 1
Li H, Borrego F, Nagata S, Tolnay M. Fc Receptor-like 5 Expression Distinguishes Two Distinct Subsets of Human Circulating Tissue-like Memory B Cells. J Immunol. 2016;196:4064-74 pubmed publisher
  • flow cytometry; human
Yu C, Becker C, Metang P, Marches F, Wang Y, Toshiyuki H, et al. Human CD141+ dendritic cells induce CD4+ T cells to produce type 2 cytokines. J Immunol. 2014;193:4335-43 pubmed publisher
  • flow cytometry; human
Marinho C, Azeredo E, Torrentes Carvalho A, Marins Dos Santos A, Kubelka C, de Souza L, et al. Down-regulation of complement receptors on the surface of host monocyte even as in vitro complement pathway blocking interferes in dengue infection. PLoS ONE. 2014;9:e102014 pubmed publisher
  • flow cytometry; human
Lee Chang C, Bodogai M, Moritoh K, Olkhanud P, Chan A, Croft M, et al. Accumulation of 4-1BBL+ B cells in the elderly induces the generation of granzyme-B+ CD8+ T cells with potential antitumor activity. Blood. 2014;124:1450-9 pubmed publisher
  • blocking or activating experiments; human
Awad A, Yassine H, Barrier M, Vorng H, Marquillies P, Tsicopoulos A, et al. Natural killer cells induce eosinophil activation and apoptosis. PLoS ONE. 2014;9:e94492 pubmed publisher
Silvin A, Chapuis N, Dunsmore G, Goubet A, Dubuisson A, Derosa L, et al. Elevated Calprotectin and Abnormal Myeloid Cell Subsets Discriminate Severe from Mild COVID-19. Cell. 2020;: pubmed publisher
Eccles J, Turner R, Kirk N, Muehling L, Borish L, Steinke J, et al. T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection. Cell Rep. 2020;30:351-366.e7 pubmed publisher
Tanner M, Pennington M, Chauhan S, Laragione T, Gulko P, Beeton C. KCa1.1 and Kv1.3 channels regulate the interactions between fibroblast-like synoviocytes and T lymphocytes during rheumatoid arthritis. Arthritis Res Ther. 2019;21:6 pubmed publisher
Zimmerman M, Quicke K, O Neal J, Arora N, Machiah D, Priyamvada L, et al. Cross-Reactive Dengue Virus Antibodies Augment Zika Virus Infection of Human Placental Macrophages. Cell Host Microbe. 2018;24:731-742.e6 pubmed publisher
Trotta A, Velásquez L, Milillo M, Delpino M, Rodriguez A, Landoni V, et al. Platelets Promote Brucella abortus Monocyte Invasion by Establishing Complexes With Monocytes. Front Immunol. 2018;9:1000 pubmed publisher
Bruno T, Ebner P, Moore B, Squalls O, Waugh K, Eruslanov E, et al. Antigen-Presenting Intratumoral B Cells Affect CD4+ TIL Phenotypes in Non-Small Cell Lung Cancer Patients. Cancer Immunol Res. 2017;5:898-907 pubmed publisher
Warncke M, Calzascia T, Coulot M, Balke N, Touil R, Kolbinger F, et al. Different adaptations of IgG effector function in human and nonhuman primates and implications for therapeutic antibody treatment. J Immunol. 2012;188:4405-11 pubmed publisher
product information
Antigen :
CD40
Apps. Abbrev. :
Flow Cytometry, Costim
Cat # :
334302
Clone :
5C3
Item :
Purified anti-human CD40
Isotype :
Mouse IgG1, κ
Other Names :
BP50, TNFRSF5
Size :
100 μg
Price (USD) :
102 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Application Notes :
Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation 1 , partial inhibition of CD40 binding to CD40L 3 , and B cell rescue from apoptosis 1 . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA