product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD163
catalog :
333602
quantity :
100 μg
price :
118 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GHI/61
reactivity :
human, mouse, rhesus macaque
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 50
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...; fig 4d, 7c
Beider K, Voevoda Dimenshtein V, Zoabi A, Rosenberg E, Magen H, Ostrovsky O, et al. CXCL13 chemokine is a novel player in multiple myeloma osteolytic microenvironment, M2 macrophage polarization, and tumor progression. J Hematol Oncol. 2022;15:144 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Jiang Y, Han Q, Zhao H, Zhang J. Promotion of epithelial-mesenchymal transformation by hepatocellular carcinoma-educated macrophages through Wnt2b/β-catenin/c-Myc signaling and reprogramming glycolysis. J Exp Clin Cancer Res. 2021;40:13 pubmed publisher
  • flow cytometry; human; loading ...; fig 3s2b
Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 3g
Stary V, Wolf B, Unterleuthner D, List J, Talic M, Laengle J, et al. Short-course radiotherapy promotes pro-inflammatory macrophages via extracellular vesicles in human rectal cancer. J Immunother Cancer. 2020;8: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 9b
Kaaij M, van Tok M, Blijdorp I, Ambarus C, Stock M, Pots D, et al. Transmembrane TNF drives osteoproliferative joint inflammation reminiscent of human spondyloarthritis. J Exp Med. 2020;217: pubmed publisher
  • flow cytometry; human; loading ...
Dupont M, Souriant S, Balboa L, Vu Manh T, Pingris K, Rousset S, et al. Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig 1c, 4b
Kim J, Jeong J, Jung J, Jeon H, Lee S, Lim J, et al. Immunological characteristics and possible pathogenic role of urinary CD11c+ macrophages in lupus nephritis. Rheumatology (Oxford). 2020;: pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Liu Q, Ou Q, Chen H, Gao Y, Liu Y, Xu Y, et al. Differential expression and predictive value of monocyte scavenger receptor CD163 in populations with different tuberculosis infection statuses. BMC Infect Dis. 2019;19:1006 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Haque A, Moriyama M, Kubota K, Ishiguro N, Sakamoto M, Chinju A, et al. CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production. Sci Rep. 2019;9:14611 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Allden S, Ogger P, Ghai P, McErlean P, Hewitt R, Toshner R, et al. The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med. 2019;: pubmed publisher
  • mass cytometry; human; loading ...; fig 2j
Wagner J, Rapsomaniki M, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer. Cell. 2019;177:1330-1345.e18 pubmed publisher
  • flow cytometry; human; 1:200; loading ...; fig 1c
Jones G, Bain C, Fenton T, Kelly A, Brown S, Ivens A, et al. Dynamics of Colon Monocyte and Macrophage Activation During Colitis. Front Immunol. 2018;9:2764 pubmed publisher
  • flow cytometry; human; loading ...; fig 1e
Bujko A, Atlasy N, Landsverk O, Richter L, Yaqub S, Horneland R, et al. Transcriptional and functional profiling defines human small intestinal macrophage subsets. J Exp Med. 2018;215:441-458 pubmed publisher
  • flow cytometry; human; fig 5b
Jeong J, Hong S, Kwon O, Ghang B, Hwang I, Kim Y, et al. CD14+ Cells with the Phenotype of Infiltrated Monocytes Consist of Distinct Populations Characterized by Anti-inflammatory as well as Pro-inflammatory Activity in Gouty Arthritis. Front Immunol. 2017;8:1260 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Vitallé J, Zenarruzabeitia O, Terrén I, Plana M, Guardo A, Leal L, et al. Monocytes Phenotype and Cytokine Production in Human Immunodeficiency Virus-1 Infected Patients Receiving a Modified Vaccinia Ankara-Based HIV-1 Vaccine: Relationship to CD300 Molecules Expression. Front Immunol. 2017;8:836 pubmed publisher
  • flow cytometry; human; 1:100; fig 5a
Beatson R, Tajadura Ortega V, Achkova D, Picco G, Tsourouktsoglou T, Klausing S, et al. The mucin MUC1 modulates the tumor immunological microenvironment through engagement of the lectin Siglec-9. Nat Immunol. 2016;17:1273-1281 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Gadd V, Patel P, Jose S, Horsfall L, Powell E, Irvine K. Altered Peripheral Blood Monocyte Phenotype and Function in Chronic Liver Disease: Implications for Hepatic Recruitment and Systemic Inflammation. PLoS ONE. 2016;11:e0157771 pubmed publisher
  • flow cytometry; human; loading ...; fig 3B
Waschbisch A, Schröder S, Schraudner D, Sammet L, Weksler B, Melms A, et al. Pivotal Role for CD16+ Monocytes in Immune Surveillance of the Central Nervous System. J Immunol. 2016;196:1558-67 pubmed publisher
  • flow cytometry; human
Cucak H, Vistisen D, Witte D, Philipsen A, Rosendahl A. Reduction of specific circulating lymphocyte populations with metabolic risk factors in patients at risk to develop type 2 diabetes. PLoS ONE. 2014;9:e107140 pubmed publisher
  • flow cytometry; rhesus macaque
Holder G, McGary C, Johnson E, Zheng R, John V, Sugimoto C, et al. Expression of the mannose receptor CD206 in HIV and SIV encephalitis: a phenotypic switch of brain perivascular macrophages with virus infection. J Neuroimmune Pharmacol. 2014;9:716-26 pubmed publisher
Zhong W, Lu Y, Han X, Yang J, Qin Z, Zhang W, et al. Upregulation of exosome secretion from tumor-associated macrophages plays a key role in the suppression of anti-tumor immunity. Cell Rep. 2023;42:113224 pubmed publisher
Wang X, Su S, Zhu Y, Cheng X, Cheng C, Chen L, et al. Metabolic Reprogramming via ACOD1 depletion enhances function of human induced pluripotent stem cell-derived CAR-macrophages in solid tumors. Nat Commun. 2023;14:5778 pubmed publisher
Warren C, Yu S, Peters D, Barbachano Guerrero A, Yang Q, Burris B, et al. Primate hemorrhagic fever-causing arteriviruses are poised for spillover to humans. Cell. 2022;185:3980-3991.e18 pubmed publisher
Gadalla R, Boukhaled G, Brooks D, Wang B. Mass cytometry immunostaining protocol for multiplexing clinical samples. STAR Protoc. 2022;3:101643 pubmed publisher
Farhadian S, Lindenbaum O, Zhao J, Corley M, Im Y, Walsh H, et al. HIV viral transcription and immune perturbations in the CNS of people with HIV despite ART. JCI Insight. 2022;7: pubmed publisher
Kim J, Li C, Wang H, Kaviraj S, Singh S, Savergave L, et al. Translational development of a tumor junction opening technology. Sci Rep. 2022;12:7753 pubmed publisher
Gómez Ferrer M, Amaro Prellezo E, Dorronsoro A, Sánchez Sánchez R, Vicente A, Cosin Roger J, et al. HIF-Overexpression and Pro-Inflammatory Priming in Human Mesenchymal Stromal Cells Improves the Healing Properties of Extracellular Vesicles in Experimental Crohn's Disease. Int J Mol Sci. 2021;22: pubmed publisher
Syrimi E, Fennell E, Richter A, Vrljicak P, Stark R, Ott S, et al. The immune landscape of SARS-CoV-2-associated Multisystem Inflammatory Syndrome in Children (MIS-C) from acute disease to recovery. iScience. 2021;24:103215 pubmed publisher
Roussel M, Ferrant J, Reizine F, Le Gallou S, Dulong J, Carl S, et al. Comparative immune profiling of acute respiratory distress syndrome patients with or without SARS-CoV-2 infection. Cell Rep Med. 2021;2:100291 pubmed publisher
Camiolo M, Zhou X, Oriss T, Yan Q, Gorry M, Horne W, et al. High-dimensional profiling clusters asthma severity by lymphoid and non-lymphoid status. Cell Rep. 2021;35:108974 pubmed publisher
Hu G, Su Y, Kang B, Fan Z, Dong T, Brown D, et al. High-throughput phenotypic screen and transcriptional analysis identify new compounds and targets for macrophage reprogramming. Nat Commun. 2021;12:773 pubmed publisher
Chevrier S, Zurbuchen Y, Cervia C, Adamo S, Raeber M, de Souza N, et al. A distinct innate immune signature marks progression from mild to severe COVID-19. Cell Rep Med. 2021;2:100166 pubmed publisher
Friebel E, Kapolou K, Unger S, Núñez N, Utz S, Rushing E, et al. Single-Cell Mapping of Human Brain Cancer Reveals Tumor-Specific Instruction of Tissue-Invading Leukocytes. Cell. 2020;181:1626-1642.e20 pubmed publisher
Pachathundikandi S, Blaser N, Bruns H, Backert S. Helicobacter pylori Avoids the Critical Activation of NLRP3 Inflammasome-Mediated Production of Oncogenic Mature IL-1β in Human Immune Cells. Cancers (Basel). 2020;12: pubmed publisher
Stras S, Werner L, Toothaker J, Olaloye O, Oldham A, McCourt C, et al. Maturation of the Human Intestinal Immune System Occurs Early in Fetal Development. Dev Cell. 2019;51:357-373.e5 pubmed publisher
Dutertre C, Becht E, Irac S, Khalilnezhad A, Narang V, Khalilnezhad S, et al. Single-Cell Analysis of Human Mononuclear Phagocytes Reveals Subset-Defining Markers and Identifies Circulating Inflammatory Dendritic Cells. Immunity. 2019;51:573-589.e8 pubmed publisher
Jordan S, Tung N, Casanova Acebes M, Chang C, Cantoni C, Zhang D, et al. Dietary Intake Regulates the Circulating Inflammatory Monocyte Pool. Cell. 2019;178:1102-1114.e17 pubmed publisher
Keskin D, Anandappa A, Sun J, Tirosh I, Mathewson N, Li S, et al. Neoantigen vaccine generates intratumoral T cell responses in phase Ib glioblastoma trial. Nature. 2019;565:234-239 pubmed publisher
Roy S, Bag A, Dutta S, Polavaram N, Islam R, Schellenburg S, et al. Macrophage-Derived Neuropilin-2 Exhibits Novel Tumor-Promoting Functions. Cancer Res. 2018;78:5600-5617 pubmed publisher
Trumpi K, Frenkel N, Peters T, Korthagen N, Jongen J, Raats D, et al. Macrophages induce "budding" in aggressive human colon cancer subtypes by protease-mediated disruption of tight junctions. Oncotarget. 2018;9:19490-19507 pubmed publisher
Genoula M, Marín Franco J, Dupont M, Kviatcovsky D, Milillo A, Schierloh P, et al. Formation of Foamy Macrophages by Tuberculous Pleural Effusions Is Triggered by the Interleukin-10/Signal Transducer and Activator of Transcription 3 Axis through ACAT Upregulation. Front Immunol. 2018;9:459 pubmed publisher
Nieto C, Bragado R, Municio C, Sierra Filardi E, Alonso B, Escribese M, et al. The Activin A-Peroxisome Proliferator-Activated Receptor Gamma Axis Contributes to the Transcriptome of GM-CSF-Conditioned Human Macrophages. Front Immunol. 2018;9:31 pubmed publisher
Sander J, Schmidt S, Cirovic B, McGovern N, Papantonopoulou O, Hardt A, et al. Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2. Immunity. 2017;47:1051-1066.e12 pubmed publisher
Rizzo M, Crawford R, Henriquez J, ALDHAMEN Y, Gulick P, Amalfitano A, et al. HIV-infected cannabis users have lower circulating CD16+ monocytes and IP-10 levels compared to non-using HIV patients. AIDS. 2017;: pubmed publisher
Cepika A, Banchereau R, Segura E, Ohouo M, Cantarel B, Goller K, et al. A multidimensional blood stimulation assay reveals immune alterations underlying systemic juvenile idiopathic arthritis. J Exp Med. 2017;214:3449-3466 pubmed publisher
Chevrier S, Levine J, Zanotelli V, Silina K, Schulz D, Bacac M, et al. An Immune Atlas of Clear Cell Renal Cell Carcinoma. Cell. 2017;169:736-749.e18 pubmed publisher
Hulsmans M, Clauss S, Xiao L, Aguirre A, King K, Hanley A, et al. Macrophages Facilitate Electrical Conduction in the Heart. Cell. 2017;169:510-522.e20 pubmed publisher
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Buttari B, Profumo E, Di Cristofano C, Pietraforte D, Lionetti V, Capoano R, et al. Haemoglobin triggers chemotaxis of human monocyte-derived dendritic cells: possible role in atherosclerotic lesion instability. Atherosclerosis. 2011;215:316-22 pubmed publisher
product information
Antigen :
CD163
Apps. Abbrev. :
FC, ICC, IP, WB
Cat # :
333602
Clone :
GHI/61
Item :
Purified anti-human CD163
Isotype :
Mouse IgG1, κ
Other Names :
GHI/61, M130, RM3/1, p155, Hemoglobin/Haptoglobin Complex Receptor, macrophage-associated antigen, ED2(rat)
Size :
100 μg
Price (USD) :
118 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Application Notes :
Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunocytochemical staining, immunoprecipitation, western blot^1, and spatial biology (IBEX)^6,7.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA