product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-human CD279 (PD-1)
catalog :
329959
quantity :
50 mg
price :
2354 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
EH12.2H7
reactivity :
human, rhesus macaque
application :
neutralization, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 35
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...; fig 1e
Jim xe9 nez Fern xe1 ndez M, Rodr xed guez Sinovas C, Ca xf1 es L, Ballester Servera C, Vara A, Requena S, et al. CD69-oxLDL ligand engagement induces Programmed Cell Death 1 (PD-1) expression in human CD4 + T lymphocytes. Cell Mol Life Sci. 2022;79:468 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b, 1d
Piao W, Li L, Saxena V, Iyyathurai J, Lakhan R, Zhang Y, et al. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration. Nat Commun. 2022;13:2176 pubmed publisher
  • flow cytometry; human; fig 3g
Dalla Pietà A, Cappuzzello E, Palmerini P, Ventura A, Visentin A, Astori G, et al. Innovative therapeutic strategy for B-cell malignancies that combines obinutuzumab and cytokine-induced killer cells. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; human; loading ...
Martínez Zamudio R, Dewald H, Vasilopoulos T, Gittens Williams L, Fitzgerald Bocarsly P, Herbig U. Senescence-associated β-galactosidase reveals the abundance of senescent CD8+ T cells in aging humans. Aging Cell. 2021;20:e13344 pubmed publisher
  • flow cytometry; human; loading ...; fig 2
Webb L, Fra Bido S, Innocentin S, Matheson L, Attaf N, Bignon A, et al. Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ. Aging Cell. 2021;20:e13295 pubmed publisher
  • flow cytometry; human; loading ...; fig 2c
Stary V, Wolf B, Unterleuthner D, List J, Talic M, Laengle J, et al. Short-course radiotherapy promotes pro-inflammatory macrophages via extracellular vesicles in human rectal cancer. J Immunother Cancer. 2020;8: pubmed publisher
  • flow cytometry; human; loading ...; fig s6d
Kuhny M, Forbes L, Çakan E, Vega Loza A, Kostiuk V, Dinesh R, et al. Disease-associated CTNNBL1 mutation impairs somatic hypermutation by decreasing nuclear AID. J Clin Invest. 2020;: pubmed publisher
  • flow cytometry; human; loading ...; fig 5b
Marasco M, Berteotti A, Weyershaeuser J, Thorausch N, Sikorska J, Krausze J, et al. Molecular mechanism of SHP2 activation by PD-1 stimulation. Sci Adv. 2020;6:eaay4458 pubmed publisher
  • flow cytometry; human; loading ...; fig s12a
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Canete P, Sweet R, Gonzalez Figueroa P, Papa I, Ohkura N, Bolton H, et al. Regulatory roles of IL-10-producing human follicular T cells. J Exp Med. 2019;: pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Thauland T, Pellerin L, Ohgami R, Bacchetta R, Butte M. Case Study: Mechanism for Increased Follicular Helper T Cell Development in Activated PI3K Delta Syndrome. Front Immunol. 2019;10:753 pubmed publisher
  • flow cytometry; human; loading ...; fig 9a
Faliti C, Gualtierotti R, Rottoli E, Gerosa M, Perruzza L, Romagnani A, et al. P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus. J Exp Med. 2019;216:317-336 pubmed publisher
  • flow cytometry; human; loading ...; fig 4a
Amelio P, Portevin D, Hella J, Reither K, Kamwela L, Lweno O, et al. HIV Infection Functionally Impairs Mycobacterium tuberculosis-Specific CD4 and CD8 T-Cell Responses. J Virol. 2019;93: pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 2a
Helmold Hait S, Vargas Inchaustegui D, Musich T, Mohanram V, Tuero I, Venzon D, et al. Early T Follicular Helper Cell Responses and Germinal Center Reactions Are Associated with Viremia Control in Immunized Rhesus Macaques. J Virol. 2019;93: pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Cai Y, Abdel Mohsen M, Tomescu C, Xue F, Wu G, Howell B, et al. BCL6 Inhibitor-Mediated Downregulation of Phosphorylated SAMHD1 and T Cell Activation Are Associated with Decreased HIV Infection and Reactivation. J Virol. 2019;93: pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 2b
Hoang T, Harper J, Pino M, Wang H, Micci L, King C, et al. Bone Marrow-Derived CD4+ T Cells Are Depleted in Simian Immunodeficiency Virus-Infected Macaques and Contribute to the Size of the Replication-Competent Reservoir. J Virol. 2019;93: pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Moysi E, Pallikkuth S, de Armas L, Gonzalez L, Ambrozak D, George V, et al. Altered immune cell follicular dynamics in HIV infection following influenza vaccination. J Clin Invest. 2018;128:3171-3185 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Wang C, Edilova M, Wagar L, Mujib S, Singer M, Bernard N, et al. Effect of IL-7 Therapy on Phospho-Ribosomal Protein S6 and TRAF1 Expression in HIV-Specific CD8 T Cells in Patients Receiving Antiretroviral Therapy. J Immunol. 2018;200:558-564 pubmed publisher
Zhang C, Cao K, Yang M, Wang Y, He M, Lu J, et al. C5aR1 blockade reshapes immunosuppressive tumor microenvironment and synergizes with immune checkpoint blockade therapy in high-grade serous ovarian cancer. Oncoimmunology. 2023;12:2261242 pubmed publisher
Wischnewski V, Maas R, Aruffo P, Soukup K, Galletti G, Kornete M, et al. Phenotypic diversity of T cells in human primary and metastatic brain tumors revealed by multiomic interrogation. Nat Cancer. 2023;4:908-924 pubmed publisher
Yang Z, Wang Y, Liu S, Deng W, Lomeli S, Moriceau G, et al. Enhancing PD-L1 Degradation by ITCH during MAPK Inhibitor Therapy Suppresses Acquired Resistance. Cancer Discov. 2022;12:1942-1959 pubmed publisher
Duraiswamy J, Turrini R, Minasyan A, Barras D, Crespo I, Grimm A, et al. Myeloid antigen-presenting cell niches sustain antitumor T cells and license PD-1 blockade via CD28 costimulation. Cancer Cell. 2021;39:1623-1642.e20 pubmed publisher
Taft J, Markson M, Legarda D, Patel R, Chan M, Malle L, et al. Human TBK1 deficiency leads to autoinflammation driven by TNF-induced cell death. Cell. 2021;184:4447-4463.e20 pubmed publisher
Zaghi E, Calvi M, Puccio S, Spata G, Terzoli S, Peano C, et al. Single-cell profiling identifies impaired adaptive NK cells expanded after HCMV reactivation in haploidentical HSCT. JCI Insight. 2021;6: pubmed publisher
Martinez M, Kim S, St Jean N, O Brien S, Lian L, Sun J, et al. Addition of anti-TIM3 or anti-TIGIT Antibodies to anti-PD1 Blockade Augments Human T cell Adoptive Cell Transfer. Oncoimmunology. 2021;10:1873607 pubmed publisher
Yang R, Mele F, Worley L, Langlais D, Rosain J, Benhsaien I, et al. Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. Cell. 2020;183:1826-1847.e31 pubmed publisher
Gruber C, Calis J, Buta S, Evrony G, Martin J, Uhl S, et al. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity. 2020;53:672-684.e11 pubmed publisher
Kim A, Han C, Driver I, Olow A, Sewell A, Zhang Z, et al. LILRB1 Blockade Enhances Bispecific T Cell Engager Antibody-Induced Tumor Cell Killing by Effector CD8+ T Cells. J Immunol. 2019;203:1076-1087 pubmed publisher
Yoshitomi H, Kobayashi S, Miyagawa Hayashino A, Okahata A, Doi K, Nishitani K, et al. Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat Commun. 2018;9:3762 pubmed publisher
Hartana C, Ahlén Bergman E, Broome A, Berglund S, Johansson M, Alamdari F, et al. Tissue-resident memory T cells are epigenetically cytotoxic with signs of exhaustion in human urinary bladder cancer. Clin Exp Immunol. 2018;194:39-53 pubmed publisher
Wang B, Zuo J, Kang W, Wei Q, Li J, Wang C, et al. Generation of Hutat2:Fc Knockin Primary Human Monocytes Using CRISPR/Cas9. Mol Ther Nucleic Acids. 2018;11:130-141 pubmed publisher
Sayin I, Radtke A, Vella L, Jin W, Wherry E, Buggert M, et al. Spatial distribution and function of T follicular regulatory cells in human lymph nodes. J Exp Med. 2018;215:1531-1542 pubmed publisher
Qaqish A, Huang D, Chen C, Zhang Z, Wang R, Li S, et al. Adoptive Transfer of Phosphoantigen-Specific ?? T Cell Subset Attenuates Mycobacterium tuberculosis Infection in Nonhuman Primates. J Immunol. 2017;198:4753-4763 pubmed publisher
Nishimura Y, Gautam R, Chun T, Sadjadpour R, Foulds K, Shingai M, et al. Early antibody therapy can induce long-lasting immunity to SHIV. Nature. 2017;543:559-563 pubmed publisher
Moon E, Ranganathan R, Eruslanov E, Kim S, Newick K, O Brien S, et al. Blockade of Programmed Death 1 Augments the Ability of Human T Cells Engineered to Target NY-ESO-1 to Control Tumor Growth after Adoptive Transfer. Clin Cancer Res. 2016;22:436-47 pubmed publisher
product information
Antigen :
CD279
Apps. Abbrev. :
FC, Block, IHC-F
Cat # :
329959
Clone :
EH12.2H7
Item :
Ultra-LEAF™ Purified anti-human CD279 (PD-1)
Isotype :
Mouse IgG1, κ
Other Names :
PD-1, PDCD1
Size :
50 mg
Price (USD) :
2354 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
ULEAF
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of ligand binding^1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections^13, and spatial biology (IBEX)^15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA