product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 700 anti-human CD279 (PD-1)
catalog :
329951
quantity :
25 tests
price :
147 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF700
clone name :
EH12.2H7
reactivity :
hamsters, human, rhesus macaque
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 71
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...; fig 1e
Jim xe9 nez Fern xe1 ndez M, Rodr xed guez Sinovas C, Ca xf1 es L, Ballester Servera C, Vara A, Requena S, et al. CD69-oxLDL ligand engagement induces Programmed Cell Death 1 (PD-1) expression in human CD4 + T lymphocytes. Cell Mol Life Sci. 2022;79:468 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b, 1d
Piao W, Li L, Saxena V, Iyyathurai J, Lakhan R, Zhang Y, et al. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration. Nat Commun. 2022;13:2176 pubmed publisher
  • flow cytometry; human; fig 3g
Dalla Pietà A, Cappuzzello E, Palmerini P, Ventura A, Visentin A, Astori G, et al. Innovative therapeutic strategy for B-cell malignancies that combines obinutuzumab and cytokine-induced killer cells. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; human; loading ...
Martínez Zamudio R, Dewald H, Vasilopoulos T, Gittens Williams L, Fitzgerald Bocarsly P, Herbig U. Senescence-associated β-galactosidase reveals the abundance of senescent CD8+ T cells in aging humans. Aging Cell. 2021;20:e13344 pubmed publisher
  • flow cytometry; human; loading ...; fig 2
Webb L, Fra Bido S, Innocentin S, Matheson L, Attaf N, Bignon A, et al. Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ. Aging Cell. 2021;20:e13295 pubmed publisher
  • flow cytometry; human; loading ...; fig 2c
Stary V, Wolf B, Unterleuthner D, List J, Talic M, Laengle J, et al. Short-course radiotherapy promotes pro-inflammatory macrophages via extracellular vesicles in human rectal cancer. J Immunother Cancer. 2020;8: pubmed publisher
  • flow cytometry; human; loading ...; fig s6d
Kuhny M, Forbes L, Çakan E, Vega Loza A, Kostiuk V, Dinesh R, et al. Disease-associated CTNNBL1 mutation impairs somatic hypermutation by decreasing nuclear AID. J Clin Invest. 2020;: pubmed publisher
  • flow cytometry; human; loading ...; fig 5b
Marasco M, Berteotti A, Weyershaeuser J, Thorausch N, Sikorska J, Krausze J, et al. Molecular mechanism of SHP2 activation by PD-1 stimulation. Sci Adv. 2020;6:eaay4458 pubmed publisher
  • flow cytometry; human; loading ...; fig s12a
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Canete P, Sweet R, Gonzalez Figueroa P, Papa I, Ohkura N, Bolton H, et al. Regulatory roles of IL-10-producing human follicular T cells. J Exp Med. 2019;: pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Thauland T, Pellerin L, Ohgami R, Bacchetta R, Butte M. Case Study: Mechanism for Increased Follicular Helper T Cell Development in Activated PI3K Delta Syndrome. Front Immunol. 2019;10:753 pubmed publisher
  • flow cytometry; human; loading ...; fig 9a
Faliti C, Gualtierotti R, Rottoli E, Gerosa M, Perruzza L, Romagnani A, et al. P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus. J Exp Med. 2019;216:317-336 pubmed publisher
  • flow cytometry; human; loading ...; fig 4a
Amelio P, Portevin D, Hella J, Reither K, Kamwela L, Lweno O, et al. HIV Infection Functionally Impairs Mycobacterium tuberculosis-Specific CD4 and CD8 T-Cell Responses. J Virol. 2019;93: pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 2a
Helmold Hait S, Vargas Inchaustegui D, Musich T, Mohanram V, Tuero I, Venzon D, et al. Early T Follicular Helper Cell Responses and Germinal Center Reactions Are Associated with Viremia Control in Immunized Rhesus Macaques. J Virol. 2019;93: pubmed publisher
  • flow cytometry; human; loading ...; fig s1d
Dias J, Boulouis C, Gorin J, van den Biggelaar R, Lal K, Gibbs A, et al. The CD4-CD8- MAIT cell subpopulation is a functionally distinct subset developmentally related to the main CD8+ MAIT cell pool. Proc Natl Acad Sci U S A. 2018;115:E11513-E11522 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Cai Y, Abdel Mohsen M, Tomescu C, Xue F, Wu G, Howell B, et al. BCL6 Inhibitor-Mediated Downregulation of Phosphorylated SAMHD1 and T Cell Activation Are Associated with Decreased HIV Infection and Reactivation. J Virol. 2019;93: pubmed publisher
  • flow cytometry; human; 1:100; loading ...
Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, et al. LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Nature. 2018;562:605-609 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 2b
Hoang T, Harper J, Pino M, Wang H, Micci L, King C, et al. Bone Marrow-Derived CD4+ T Cells Are Depleted in Simian Immunodeficiency Virus-Infected Macaques and Contribute to the Size of the Replication-Competent Reservoir. J Virol. 2019;93: pubmed publisher
  • flow cytometry; human; loading ...; fig 3d
Hartana C, Ahlén Bergman E, Broome A, Berglund S, Johansson M, Alamdari F, et al. Tissue-resident memory T cells are epigenetically cytotoxic with signs of exhaustion in human urinary bladder cancer. Clin Exp Immunol. 2018;194:39-53 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Moysi E, Pallikkuth S, de Armas L, Gonzalez L, Ambrozak D, George V, et al. Altered immune cell follicular dynamics in HIV infection following influenza vaccination. J Clin Invest. 2018;128:3171-3185 pubmed publisher
  • immunohistochemistry - frozen section; human; fig 1a
  • flow cytometry; human; fig 1b
Sayin I, Radtke A, Vella L, Jin W, Wherry E, Buggert M, et al. Spatial distribution and function of T follicular regulatory cells in human lymph nodes. J Exp Med. 2018;215:1531-1542 pubmed publisher
  • flow cytometry; hamsters; loading ...; fig s5
Adler A, Bedinger D, Adams M, Asensio M, Edgar R, Leong R, et al. A natively paired antibody library yields drug leads with higher sensitivity and specificity than a randomly paired antibody library. MAbs. 2018;10:431-443 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Wang C, Edilova M, Wagar L, Mujib S, Singer M, Bernard N, et al. Effect of IL-7 Therapy on Phospho-Ribosomal Protein S6 and TRAF1 Expression in HIV-Specific CD8 T Cells in Patients Receiving Antiretroviral Therapy. J Immunol. 2018;200:558-564 pubmed publisher
  • flow cytometry; human; loading ...; fig s2a
van der Geest K, Wang Q, Eijsvogels T, Koenen H, Joosten I, Brouwer E, et al. Changes in peripheral immune cell numbers and functions in octogenarian walkers - an acute exercise study. Immun Ageing. 2017;14:5 pubmed publisher
Lehman N, Kowalska W, Zarobkiewicz M, Mazurek M, Mrozowska K, Bojarska Junak A, et al. Pro- vs. Anti-Inflammatory Features of Monocyte Subsets in Glioma Patients. Int J Mol Sci. 2023;24: pubmed publisher
Anadon C, Yu X, Hänggi K, Biswas S, Chaurio R, Martin A, et al. Ovarian cancer immunogenicity is governed by a narrow subset of progenitor tissue-resident memory T cells. Cancer Cell. 2022;40:545-557.e13 pubmed publisher
Ford B, Moncada Giraldo D, Margaroli C, Giacalone V, Brown M, Peng L, et al. Functional and Transcriptional Adaptations of Blood Monocytes Recruited to the Cystic Fibrosis Airway Microenvironment In Vitro. Int J Mol Sci. 2021;22: pubmed publisher
Abd Hamid M, Colin York H, Khalid Alham N, Browne M, Cerundolo L, Chen J, et al. Self-Maintaining CD103+ Cancer-Specific T Cells Are Highly Energetic with Rapid Cytotoxic and Effector Responses. Cancer Immunol Res. 2020;8:203-216 pubmed publisher
Huang B, Chen Z, Geng L, Wang J, Liang H, Cao Y, et al. Mucosal Profiling of Pediatric-Onset Colitis and IBD Reveals Common Pathogenics and Therapeutic Pathways. Cell. 2019;179:1160-1176.e24 pubmed publisher
Kim A, Han C, Driver I, Olow A, Sewell A, Zhang Z, et al. LILRB1 Blockade Enhances Bispecific T Cell Engager Antibody-Induced Tumor Cell Killing by Effector CD8+ T Cells. J Immunol. 2019;203:1076-1087 pubmed publisher
Mintz M, Felce J, Chou M, Mayya V, Xu Y, Shui J, et al. The HVEM-BTLA Axis Restrains T Cell Help to Germinal Center B Cells and Functions as a Cell-Extrinsic Suppressor in Lymphomagenesis. Immunity. 2019;: pubmed publisher
Celis Gutierrez J, Blattmann P, Zhai Y, Jarmuzynski N, Ruminski K, Gregoire C, et al. Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy. Cell Rep. 2019;27:3315-3330.e7 pubmed publisher
Wagner J, Rapsomaniki M, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer. Cell. 2019;177:1330-1345.e18 pubmed publisher
Gong B, Kiyotani K, Sakata S, Nagano S, Kumehara S, Baba S, et al. Secreted PD-L1 variants mediate resistance to PD-L1 blockade therapy in non-small cell lung cancer. J Exp Med. 2019;: pubmed publisher
Lu E, Wolfreys F, Muppidi J, Xu Y, Cyster J. S-Geranylgeranyl-L-glutathione is a ligand for human B cell-confinement receptor P2RY8. Nature. 2019;: pubmed publisher
Sueyoshi K, Ledderose C, Shen Y, Lee A, Shapiro N, Junger W. Lipopolysaccharide suppresses T cells by generating extracellular ATP that impairs their mitochondrial function via P2Y11 receptors. J Biol Chem. 2019;294:6283-6293 pubmed publisher
Wu X, Li Y, Liu X, Chen C, Harrington S, Cao S, et al. Targeting B7-H1 (PD-L1) sensitizes cancer cells to chemotherapy. Heliyon. 2018;4:e01039 pubmed publisher
Stoeckius M, Zheng S, Houck Loomis B, Hao S, Yeung B, Mauck W, et al. Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics. Genome Biol. 2018;19:224 pubmed publisher
Chee J, Watson M, Chopra A, Nguyen B, Cook A, Creaney J, et al. Tumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptors. BMC Res Notes. 2018;11:864 pubmed publisher
Andre P, Denis C, Soulas C, Bourbon Caillet C, Lopez J, Arnoux T, et al. Anti-NKG2A mAb Is a Checkpoint Inhibitor that Promotes Anti-tumor Immunity by Unleashing Both T and NK Cells. Cell. 2018;175:1731-1743.e13 pubmed publisher
Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed publisher
Maity A, Mick R, Huang A, George S, Farwell M, Lukens J, et al. A phase I trial of pembrolizumab with hypofractionated radiotherapy in patients with metastatic solid tumours. Br J Cancer. 2018;119:1200-1207 pubmed publisher
Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed publisher
Wong Y, Joshi K, Khetrapal P, Ismail M, Reading J, Sunderland M, et al. Urine-derived lymphocytes as a non-invasive measure of the bladder tumor immune microenvironment. J Exp Med. 2018;215:2748-2759 pubmed publisher
Xu H, Ziani W, Shao J, Doyle Meyers L, Russell Lodrigue K, Ratterree M, et al. Impaired Development and Expansion of Germinal Center Follicular Th Cells in Simian Immunodeficiency Virus-Infected Neonatal Macaques. J Immunol. 2018;201:1994-2003 pubmed publisher
Burton A, Pallett L, McCoy L, Suveizdyte K, Amin O, Swadling L, et al. Circulating and intrahepatic antiviral B cells are defective in hepatitis B. J Clin Invest. 2018;128:4588-4603 pubmed publisher
Kim S, Choi J, Laínez B, Schulz V, Karas D, Baum E, et al. Human Extrafollicular CD4+ Th Cells Help Memory B Cells Produce Igs. J Immunol. 2018;201:1359-1372 pubmed publisher
Mavigner M, Habib J, Deléage C, Rosen E, Mattingly C, Bricker K, et al. Simian Immunodeficiency Virus Persistence in Cellular and Anatomic Reservoirs in Antiretroviral Therapy-Suppressed Infant Rhesus Macaques. J Virol. 2018;92: pubmed publisher
Tang D, Zhao D, Wu Y, Yao R, Zhou L, Lu L, et al. The miR-3127-5p/p-STAT3 axis up-regulates PD-L1 inducing chemoresistance in non-small-cell lung cancer. J Cell Mol Med. 2018;: pubmed publisher
Wilson J, Zhao Y, Singer M, Spencer J, Shankar Hari M. Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study. Crit Care. 2018;22:95 pubmed publisher
Gee M, Han A, Lofgren S, Beausang J, Mendoza J, Birnbaum M, et al. Antigen Identification for Orphan T Cell Receptors Expressed on Tumor-Infiltrating Lymphocytes. Cell. 2018;172:549-563.e16 pubmed publisher
Nissim L, Wu M, Pery E, Binder Nissim A, Suzuki H, Stupp D, et al. Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer Immunotherapy. Cell. 2017;171:1138-1150.e15 pubmed publisher
Powell R, Lissauer D, Tamblyn J, Beggs A, Cox P, Moss P, et al. Decidual T Cells Exhibit a Highly Differentiated Phenotype and Demonstrate Potential Fetal Specificity and a Strong Transcriptional Response to IFN. J Immunol. 2017;199:3406-3417 pubmed publisher
Vargas Inchaustegui D, Helmold Hait S, Chung H, Narola J, Hoang T, Robert Guroff M. Phenotypic and Functional Characterization of Circulatory, Splenic, and Hepatic NK Cells in Simian Immunodeficiency Virus-Controlling Macaques. J Immunol. 2017;199:3202-3211 pubmed publisher
Teigler J, Zelinskyy G, Eller M, Bolton D, Marovich M, Gordon A, et al. Differential Inhibitory Receptor Expression on T Cells Delineates Functional Capacities in Chronic Viral Infection. J Virol. 2017;91: pubmed publisher
Day C, Abrahams D, Harris L, van Rooyen M, Stone L, de Kock M, et al. HIV-1 Infection Is Associated with Depletion and Functional Impairment of Mycobacterium tuberculosis-Specific CD4 T Cells in Individuals with Latent Tuberculosis Infection. J Immunol. 2017;199:2069-2080 pubmed publisher
Cobb D, Kim O, Golden Mason L, Rosen H, Hahn Y. Hepatocyte-derived exosomes promote T follicular regulatory cell expansion during hepatitis C virus infection. Hepatology. 2017;: pubmed publisher
Pan T, Zhou T, Li L, Liu Z, Chen Y, Mao E, et al. Monocyte programmed death ligand-1 expression is an early marker for predicting infectious complications in acute pancreatitis. Crit Care. 2017;21:186 pubmed publisher
Papa I, Saliba D, Ponzoni M, Bustamante S, Canete P, Gonzalez Figueroa P, et al. TFH-derived dopamine accelerates productive synapses in germinal centres. Nature. 2017;547:318-323 pubmed publisher
Weinberg A, Canniff J, Rouphael N, Mehta A, Mulligan M, Whitaker J, et al. Varicella-Zoster Virus-Specific Cellular Immune Responses to the Live Attenuated Zoster Vaccine in Young and Older Adults. J Immunol. 2017;199:604-612 pubmed publisher
Chevrier S, Levine J, Zanotelli V, Silina K, Schulz D, Bacac M, et al. An Immune Atlas of Clear Cell Renal Cell Carcinoma. Cell. 2017;169:736-749.e18 pubmed publisher
Kailayangiri S, Altvater B, Spurny C, Jamitzky S, Schelhaas S, Jacobs A, et al. Targeting Ewing sarcoma with activated and GD2-specific chimeric antigen receptor-engineered human NK cells induces upregulation of immune-inhibitory HLA-G. Oncoimmunology. 2017;6:e1250050 pubmed publisher
Mylvaganam G, Rios D, Abdelaal H, Iyer S, Tharp G, Mavigner M, et al. Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection. Proc Natl Acad Sci U S A. 2017;114:1976-1981 pubmed publisher
Rancan C, Schirrmann L, Hüls C, Zeidler R, Moosmann A. Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells. PLoS Pathog. 2015;11:e1004906 pubmed publisher
Conrad J, Ramalingam R, Smith R, Barnett L, Lorey S, Wei J, et al. Dominant clonotypes within HIV-specific T cell responses are programmed death-1high and CD127low and display reduced variant cross-reactivity. J Immunol. 2011;186:6871-85 pubmed publisher
Monteiro P, Gosselin A, Wacleche V, El Far M, Said E, Kared H, et al. Memory CCR6+CD4+ T cells are preferential targets for productive HIV type 1 infection regardless of their expression of integrin ?7. J Immunol. 2011;186:4618-30 pubmed publisher
Radziewicz H, Ibegbu C, Hon H, Bedard N, Bruneau J, Workowski K, et al. Transient CD86 expression on hepatitis C virus-specific CD8+ T cells in acute infection is linked to sufficient IL-2 signaling. J Immunol. 2010;184:2410-22 pubmed publisher
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Chang W, Kim J, Kim Y, Kim Y, Lee J, Azuma M, et al. Cutting edge: Programmed death-1/programmed death ligand 1 interaction regulates the induction and maintenance of invariant NKT cell anergy. J Immunol. 2008;181:6707-10 pubmed
Zahn R, Rett M, Korioth Schmitz B, Sun Y, Buzby A, Goldstein S, et al. Simian immunodeficiency virus (SIV)-specific CD8+ T-cell responses in vervet African green monkeys chronically infected with SIVagm. J Virol. 2008;82:11577-88 pubmed publisher
product information
Antigen :
CD279
Apps. Abbrev. :
FC
Cat # :
329951
Clone :
EH12.2H7
Item :
Alexa Fluor® 700 anti-human CD279 (PD-1)
Isotype :
Mouse IgG1, κ
Other Names :
PD-1, PDCD1
Size :
25 tests
Price (USD) :
147 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A700
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of ligand binding^1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections^13, and spatial biology (IBEX)^15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA