product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 421™ anti-human CD279 (PD-1)
catalog :
329919
quantity :
25 tests
price :
185 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV421
clone name :
EH12.2H7
reactivity :
human, mouse, chimpanzee, rhesus macaque
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 51
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s1
Canete P, Sweet R, Gonzalez Figueroa P, Papa I, Ohkura N, Bolton H, et al. Regulatory roles of IL-10-producing human follicular T cells. J Exp Med. 2019;: pubmed publisher
  • flow cytometry; human; loading ...; fig 4a
Amelio P, Portevin D, Hella J, Reither K, Kamwela L, Lweno O, et al. HIV Infection Functionally Impairs Mycobacterium tuberculosis-Specific CD4 and CD8 T-Cell Responses. J Virol. 2019;93: pubmed publisher
  • flow cytometry; human; loading ...; fig s1d
Dias J, Boulouis C, Gorin J, van den Biggelaar R, Lal K, Gibbs A, et al. The CD4-CD8- MAIT cell subpopulation is a functionally distinct subset developmentally related to the main CD8+ MAIT cell pool. Proc Natl Acad Sci U S A. 2018;115:E11513-E11522 pubmed publisher
  • immunohistochemistry - frozen section; human; fig 1a
  • flow cytometry; human; fig 1b
Sayin I, Radtke A, Vella L, Jin W, Wherry E, Buggert M, et al. Spatial distribution and function of T follicular regulatory cells in human lymph nodes. J Exp Med. 2018;215:1531-1542 pubmed publisher
  • flow cytometry; rhesus macaque; fig s4f
Mylvaganam G, Rios D, Abdelaal H, Iyer S, Tharp G, Mavigner M, et al. Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection. Proc Natl Acad Sci U S A. 2017;114:1976-1981 pubmed publisher
  • flow cytometry; human; loading ...
Tauriainen J, Scharf L, Frederiksen J, Naji A, Ljunggren H, Sonnerborg A, et al. Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals. Sci Rep. 2017;7:40354 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Cheng L, Ma J, Li J, Li D, Li G, Li F, et al. Blocking type I interferon signaling enhances T cell recovery and reduces HIV-1 reservoirs. J Clin Invest. 2017;127:269-279 pubmed publisher
  • flow cytometry; human; fig 1a
Paris R, Petrovas C, Ferrando Martinez S, Moysi E, Boswell K, Archer E, et al. Selective Loss of Early Differentiated, Highly Functional PD1high CD4 T Cells with HIV Progression. PLoS ONE. 2015;10:e0144767 pubmed publisher
  • flow cytometry; human; fig 1
Frederiksen J, Buggert M, Noyan K, Nowak P, Sönnerborg A, Lund O, et al. Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection. PLoS ONE. 2015;10:e0137635 pubmed publisher
  • flow cytometry; human
Lee J, Jeong I, Joh J, Jung Y, Sim S, Choi B, et al. Differential expression of CD57 in antigen-reactive CD4+ T cells between active and latent tuberculosis infection. Clin Immunol. 2015;159:37-46 pubmed publisher
  • flow cytometry; human
Buggert M, Tauriainen J, Yamamoto T, Frederiksen J, Ivarsson M, Michaelsson J, et al. T-bet and Eomes are differentially linked to the exhausted phenotype of CD8+ T cells in HIV infection. PLoS Pathog. 2014;10:e1004251 pubmed publisher
Bradley T, Kuraoka M, Yeh C, Tian M, Chen H, Cain D, et al. Immune checkpoint modulation enhances HIV-1 antibody induction. Nat Commun. 2020;11:948 pubmed publisher
Han Q, Bradley T, Williams W, Cain D, Montefiori D, Saunders K, et al. Neonatal Rhesus Macaques Have Distinct Immune Cell Transcriptional Profiles following HIV Envelope Immunization. Cell Rep. 2020;30:1553-1569.e6 pubmed publisher
Cui J, Wang H, Medina R, Zhang Q, Xu C, Indig I, et al. Inhibition of PP2A with LB-100 Enhances Efficacy of CAR-T Cell Therapy Against Glioblastoma. Cancers (Basel). 2020;12: pubmed publisher
Mastelic Gavillet B, Navarro Rodrigo B, Décombaz L, Wang H, Ercolano G, Ahmed R, et al. Adenosine mediates functional and metabolic suppression of peripheral and tumor-infiltrating CD8+ T cells. J Immunother Cancer. 2019;7:257 pubmed publisher
Leng T, Akther H, Hackstein C, Powell K, King T, Friedrich M, et al. TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions. Cell Rep. 2019;28:3077-3091.e5 pubmed publisher
Claiborne D, Scully E, Palmer C, Prince J, Macharia G, Kopycinski J, et al. Protective HLA alleles are associated with reduced LPS levels in acute HIV infection with implications for immune activation and pathogenesis. PLoS Pathog. 2019;15:e1007981 pubmed publisher
Ollé Hurtado M, Wolbert J, Fisher J, Flutter B, Stafford S, Barton J, et al. Tumor infiltrating lymphocytes expanded from pediatric neuroblastoma display heterogeneity of phenotype and function. PLoS ONE. 2019;14:e0216373 pubmed publisher
Celis Gutierrez J, Blattmann P, Zhai Y, Jarmuzynski N, Ruminski K, Gregoire C, et al. Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy. Cell Rep. 2019;27:3315-3330.e7 pubmed publisher
Collinson Pautz M, Chang W, Lu A, Khalil M, Crisostomo J, Lin P, et al. Constitutively active MyD88/CD40 costimulation enhances expansion and efficacy of chimeric antigen receptor T cells targeting hematological malignancies. Leukemia. 2019;: pubmed publisher
Herrera F, Valerio M, Berthold D, Tawadros T, Meuwly J, Vallet V, et al. 50-Gy Stereotactic Body Radiation Therapy to the Dominant Intraprostatic Nodule: Results From a Phase 1a/b Trial. Int J Radiat Oncol Biol Phys. 2019;103:320-334 pubmed publisher
Whitney J, Lim S, Osuna C, Kublin J, Chen E, Yoon G, et al. Prevention of SIVmac251 reservoir seeding in rhesus monkeys by early antiretroviral therapy. Nat Commun. 2018;9:5429 pubmed publisher
Andre P, Denis C, Soulas C, Bourbon Caillet C, Lopez J, Arnoux T, et al. Anti-NKG2A mAb Is a Checkpoint Inhibitor that Promotes Anti-tumor Immunity by Unleashing Both T and NK Cells. Cell. 2018;175:1731-1743.e13 pubmed publisher
Minagawa A, Yoshikawa T, Yasukawa M, Hotta A, Kunitomo M, Iriguchi S, et al. Enhancing T Cell Receptor Stability in Rejuvenated iPSC-Derived T Cells Improves Their Use in Cancer Immunotherapy. Cell Stem Cell. 2018;23:850-858.e4 pubmed publisher
Sade Feldman M, Yizhak K, Bjorgaard S, Ray J, de Boer C, Jenkins R, et al. Defining T Cell States Associated with Response to Checkpoint Immunotherapy in Melanoma. Cell. 2018;175:998-1013.e20 pubmed publisher
Levin M, Kroehl M, Johnson M, Hammes A, Reinhold D, Lang N, et al. Th1 memory differentiates recombinant from live herpes zoster vaccines. J Clin Invest. 2018;128:4429-4440 pubmed publisher
Bengsch B, Ohtani T, Khan O, Setty M, Manne S, O Brien S, et al. Epigenomic-Guided Mass Cytometry Profiling Reveals Disease-Specific Features of Exhausted CD8 T Cells. Immunity. 2018;48:1029-1045.e5 pubmed publisher
Ferrando Martinez S, Moysi E, Pegu A, Andrews S, Nganou Makamdop K, Ambrozak D, et al. Accumulation of follicular CD8+ T cells in pathogenic SIV infection. J Clin Invest. 2018;128:2089-2103 pubmed publisher
Lee S, Adriani G, Ceccarello E, Pavesi A, Tan A, Bertoletti A, et al. Characterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model. Front Immunol. 2018;9:416 pubmed publisher
Cao J, Perez Pinera P, Lowenhaupt K, Wu M, Purcell O, de la Fuente Nunez C, et al. Versatile and on-demand biologics co-production in yeast. Nat Commun. 2018;9:77 pubmed publisher
Nissim L, Wu M, Pery E, Binder Nissim A, Suzuki H, Stupp D, et al. Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer Immunotherapy. Cell. 2017;171:1138-1150.e15 pubmed publisher
Baxter A, Niessl J, Fromentin R, Richard J, Porichis F, Massanella M, et al. Multiparametric characterization of rare HIV-infected cells using an RNA-flow FISH technique. Nat Protoc. 2017;12:2029-2049 pubmed publisher
Rutishauser R, Hartogensis W, Deguit C, Krone M, Hoh R, Hecht F, et al. Early and Delayed Antiretroviral Therapy Results in Comparable Reductions in CD8+ T Cell Exhaustion Marker Expression. AIDS Res Hum Retroviruses. 2017;33:658-667 pubmed publisher
Huang R, Francois A, McGray A, Miliotto A, Odunsi K. Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer. Oncoimmunology. 2017;6:e1249561 pubmed publisher
Pilkinton M, Nicholas K, Warren C, Smith R, Yoder S, Talbot H, et al. Greater activation of peripheral T follicular helper cells following high dose influenza vaccine in older adults forecasts seroconversion. Vaccine. 2017;35:329-336 pubmed publisher
Belkina A, Snyder Cappione J. OMIP-037: 16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets. Cytometry A. 2017;91:175-179 pubmed publisher
Calascibetta F, Micci L, Carnathan D, Lawson B, Vanderford T, Bosinger S, et al. Antiretroviral Therapy in Simian Immunodeficiency Virus-Infected Sooty Mangabeys: Implications for AIDS Pathogenesis. J Virol. 2016;90:7541-7551 pubmed publisher
Nicholas K, Greenplate A, Flaherty D, Matlock B, Juan J, Smith R, et al. Multiparameter analysis of stimulated human peripheral blood mononuclear cells: A comparison of mass and fluorescence cytometry. Cytometry A. 2016;89:271-80 pubmed publisher
Eberhardt K, Sarfo F, Dompreh A, Kuffour E, Geldmacher C, Soltau M, et al. Helicobacter pylori Coinfection Is Associated With Decreased Markers of Immune Activation in ART-Naive HIV-Positive and in HIV-Negative Individuals in Ghana. Clin Infect Dis. 2015;61:1615-23 pubmed publisher
Claiborne D, Prince J, Scully E, Macharia G, Micci L, Lawson B, et al. Replicative fitness of transmitted HIV-1 drives acute immune activation, proviral load in memory CD4+ T cells, and disease progression. Proc Natl Acad Sci U S A. 2015;112:E1480-9 pubmed publisher
Mylvaganam G, Velu V, Hong J, Sadagopal S, Kwa S, Basu R, et al. Diminished viral control during simian immunodeficiency virus infection is associated with aberrant PD-1hi CD4 T cell enrichment in the lymphoid follicles of the rectal mucosa. J Immunol. 2014;193:4527-36 pubmed publisher
Buggert M, Norstr m M, Salemi M, Hecht F, Karlsson A. Functional avidity and IL-2/perforin production is linked to the emergence of mutations within HLA-B*5701-restricted epitopes and HIV-1 disease progression. J Immunol. 2014;192:4685-96 pubmed publisher
Buggert M, Frederiksen J, Noyan K, Svärd J, Barqasho B, Sonnerborg A, et al. Multiparametric bioinformatics distinguish the CD4/CD8 ratio as a suitable laboratory predictor of combined T cell pathogenesis in HIV infection. J Immunol. 2014;192:2099-108 pubmed publisher
Pacheco Y, McLean A, Rohrbach J, Porichis F, Kaufmann D, Kavanagh D. Simultaneous TCR and CD244 signals induce dynamic downmodulation of CD244 on human antiviral T cells. J Immunol. 2013;191:2072-81 pubmed publisher
Conrad J, Ramalingam R, Smith R, Barnett L, Lorey S, Wei J, et al. Dominant clonotypes within HIV-specific T cell responses are programmed death-1high and CD127low and display reduced variant cross-reactivity. J Immunol. 2011;186:6871-85 pubmed publisher
Monteiro P, Gosselin A, Wacleche V, El Far M, Said E, Kared H, et al. Memory CCR6+CD4+ T cells are preferential targets for productive HIV type 1 infection regardless of their expression of integrin ?7. J Immunol. 2011;186:4618-30 pubmed publisher
Radziewicz H, Ibegbu C, Hon H, Bedard N, Bruneau J, Workowski K, et al. Transient CD86 expression on hepatitis C virus-specific CD8+ T cells in acute infection is linked to sufficient IL-2 signaling. J Immunol. 2010;184:2410-22 pubmed publisher
Vojnov L, Reed J, Weisgrau K, Rakasz E, Loffredo J, Piaskowski S, et al. Effective simian immunodeficiency virus-specific CD8+ T cells lack an easily detectable, shared characteristic. J Virol. 2010;84:753-64 pubmed publisher
Jones R, Yue F, Gu X, Hunter D, Mujib S, Gyenes G, et al. Human immunodeficiency virus type 1 escapes from interleukin-2-producing CD4+ T-cell responses without high-frequency fixation of mutations. J Virol. 2009;83:8722-32 pubmed publisher
Chang W, Kim J, Kim Y, Kim Y, Lee J, Azuma M, et al. Cutting edge: Programmed death-1/programmed death ligand 1 interaction regulates the induction and maintenance of invariant NKT cell anergy. J Immunol. 2008;181:6707-10 pubmed
Zahn R, Rett M, Korioth Schmitz B, Sun Y, Buzby A, Goldstein S, et al. Simian immunodeficiency virus (SIV)-specific CD8+ T-cell responses in vervet African green monkeys chronically infected with SIVagm. J Virol. 2008;82:11577-88 pubmed publisher
product information
Antigen :
CD279
Apps. Abbrev. :
Flow Cytometry
Cat # :
329919
Clone :
EH12.2H7
Item :
Brilliant Violet 421™ anti-human CD279 (PD-1)
Isotype :
Mouse IgG1, κ
Other Names :
PD-1
Size :
25 tests
Price (USD) :
185 USD
Reactivity :
Human, African Green, Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Rhesus, Squirrel Monkey
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV421
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of ligand binding 1-3 and immunohistochemical staining of paraformaldehyde fixed frozen sections 13 . The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA