LEAF™ Purified anti-human CD279 (PD-1) | BioLegend 329912 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD279 (PD-1)

catalog :

329912

quantity :

500 μg

price :

375 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

EH12.2H7

reactivity :

African green monkey, hamsters, human, mouse, chimpanzee, Rhesus monkey

application :

immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

citations: 34

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig s1d	Dias J, Boulouis C, Gorin J, van den Biggelaar R, Lal K, Gibbs A, et al. The CD4-CD8- MAIT cell subpopulation is a functionally distinct subset developmentally related to the main CD8+ MAIT cell pool. Proc Natl Acad Sci U S A. 2018;115:E11513-E11522 pubmed publisher
flow cytometry; human; 1:100; loading ...	Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, et al. LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Nature. 2018;562:605-609 pubmed publisher
flow cytometry; human; loading ...; fig 3d	Hartana C, Ahlén Bergman E, Broome A, Berglund S, Johansson M, Alamdari F, et al. Tissue-resident memory T cells are epigenetically cytotoxic with signs of exhaustion in human urinary bladder cancer. Clin Exp Immunol. 2018;194:39-53 pubmed publisher
immunohistochemistry - frozen section; human; fig 1a flow cytometry; human; fig 1b	Sayin I, Radtke A, Vella L, Jin W, Wherry E, Buggert M, et al. Spatial distribution and function of T follicular regulatory cells in human lymph nodes. J Exp Med. 2018;215:1531-1542 pubmed publisher
flow cytometry; hamsters; loading ...; fig s5	Adler A, Bedinger D, Adams M, Asensio M, Edgar R, Leong R, et al. A natively paired antibody library yields drug leads with higher sensitivity and specificity than a randomly paired antibody library. MAbs. 2018;10:431-443 pubmed publisher
flow cytometry; human; loading ...; fig 5b	Gaggianesi M, Turdo A, Chinnici A, Lipari E, Apuzzo T, Benfante A, et al. IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition. Cancer Res. 2017;77:3268-3279 pubmed publisher
flow cytometry; human; loading ...; fig s2a	van der Geest K, Wang Q, Eijsvogels T, Koenen H, Joosten I, Brouwer E, et al. Changes in peripheral immune cell numbers and functions in octogenarian walkers - an acute exercise study. Immun Ageing. 2017;14:5 pubmed publisher
immunohistochemistry - frozen section; Rhesus monkey; loading ...; fig s3a flow cytometry; Rhesus monkey	Mylvaganam G, Rios D, Abdelaal H, Iyer S, Tharp G, Mavigner M, et al. Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection. Proc Natl Acad Sci U S A. 2017;114:1976-1981 pubmed publisher
flow cytometry; human; loading ...	Tauriainen J, Scharf L, Frederiksen J, Naji A, Ljunggren H, Sonnerborg A, et al. Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals. Sci Rep. 2017;7:40354 pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Cheng L, Ma J, Li J, Li D, Li G, Li F, et al. Blocking type I interferon signaling enhances T cell recovery and reduces HIV-1 reservoirs. J Clin Invest. 2017;127:269-279 pubmed publisher
flow cytometry; human; loading ...; fig 3b	Ashizawa T, Iizuka A, Nonomura C, Kondou R, Maeda C, Miyata H, et al. Antitumor Effect of Programmed Death-1 (PD-1) Blockade in Humanized the NOG-MHC Double Knockout Mouse. Clin Cancer Res. 2017;23:149-158 pubmed publisher
flow cytometry; human; loading ...; fig 3	Zwang N, Zhang R, Germana S, Fan M, Hastings W, Cao A, et al. Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways. Am J Transplant. 2016;16:2624-38 pubmed publisher
flow cytometry; human; fig 4, 5	Yasuma K, Yasunaga J, Takemoto K, Sugata K, Mitobe Y, Takenouchi N, et al. HTLV-1 bZIP Factor Impairs Anti-viral Immunity by Inducing Co-inhibitory Molecule, T Cell Immunoglobulin and ITIM Domain (TIGIT). PLoS Pathog. 2016;12:e1005372 pubmed publisher
flow cytometry; human; fig 1a	Paris R, Petrovas C, Ferrando Martinez S, Moysi E, Boswell K, Archer E, et al. Selective Loss of Early Differentiated, Highly Functional PD1high CD4 T Cells with HIV Progression. PLoS ONE. 2015;10:e0144767 pubmed publisher
blocking or activating experiments; human; fig 8	Rancan C, Schirrmann L, Hüls C, Zeidler R, Moosmann A. Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells. PLoS Pathog. 2015;11:e1004906 pubmed publisher
flow cytometry; human	Pombo C, Wherry E, Gostick E, Price D, Betts M. Elevated Expression of CD160 and 2B4 Defines a Cytolytic HIV-Specific CD8+ T-Cell Population in Elite Controllers. J Infect Dis. 2015;212:1376-86 pubmed publisher
flow cytometry; African green monkey; fig s4a	Gideon H, Phuah J, Myers A, Bryson B, Rodgers M, Coleman M, et al. Variability in tuberculosis granuloma T cell responses exists, but a balance of pro- and anti-inflammatory cytokines is associated with sterilization. PLoS Pathog. 2015;11:e1004603 pubmed publisher
flow cytometry; human	Sawada Y, Yoshikawa T, Shimomura M, Iwama T, Endo I, Nakatsura T. Programmed death-1 blockade enhances the antitumor effects of peptide vaccine-induced peptide-specific cytotoxic TÂ lymphocytes. Int J Oncol. 2015;46:28-36 pubmed publisher
flow cytometry; Rhesus monkey	Fujita T, Burwitz B, Chew G, Reed J, Pathak R, Seger E, et al. Expansion of dysfunctional Tim-3-expressing effector memory CD8+ T cells during simian immunodeficiency virus infection in rhesus macaques. J Immunol. 2014;193:5576-83 pubmed publisher
blocking or activating experiments; human	Langhans B, Alwan A, KrÃ¤mer B, GlÃ¤ssner A, Lutz P, Strassburg C, et al. Regulatory CD4+ T cells modulate the interaction between NK cells and hepatic stellate cells by acting on either cell type. J Hepatol. 2015;62:398-404 pubmed publisher
flow cytometry; human	Herati R, Reuter M, Dolfi D, Mansfield K, Aung H, Badwan O, et al. Circulating CXCR5+PD-1+ response predicts influenza vaccine antibody responses in young adults but not elderly adults. J Immunol. 2014;193:3528-37 pubmed publisher
flow cytometry; human	Bending D, Pesenacker A, Ursu S, Wu Q, Lom H, Thirugnanabalan B, et al. Hypomethylation at the regulatory T cell-specific demethylated region in CD25hi T cells is decoupled from FOXP3 expression at the inflamed site in childhood arthritis. J Immunol. 2014;193:2699-708 pubmed publisher
immunocytochemistry; human	Gautron A, Dominguez Villar M, de Marcken M, Hafler D. Enhanced suppressor function of TIM-3+ FoxP3+ regulatory T cells. Eur J Immunol. 2014;44:2703-2711 pubmed publisher
flow cytometry; human	Buggert M, Norstr m M, Salemi M, Hecht F, Karlsson A. Functional avidity and IL-2/perforin production is linked to the emergence of mutations within HLA-B*5701-restricted epitopes and HIV-1 disease progression. J Immunol. 2014;192:4685-96 pubmed publisher
flow cytometry; human; 0.7:100	Gros A, Robbins P, Yao X, Li Y, Turcotte S, Tran E, et al. PD-1 identifies the patient-specific CD8? tumor-reactive repertoire infiltrating human tumors. J Clin Invest. 2014;124:2246-59 pubmed publisher
flow cytometry; human	Lanteri M, Diamond M, Law J, Chew G, Wu S, Inglis H, et al. Increased frequency of Tim-3 expressing T cells is associated with symptomatic West Nile virus infection. PLoS ONE. 2014;9:e92134 pubmed publisher
flow cytometry; human	Radvanyi L, Bernatchez C, Zhang M, Fox P, Miller P, Chacon J, et al. Specific lymphocyte subsets predict response to adoptive cell therapy using expanded autologous tumor-infiltrating lymphocytes in metastatic melanoma patients. Clin Cancer Res. 2012;18:6758-70 pubmed publisher
	Conrad J, Ramalingam R, Smith R, Barnett L, Lorey S, Wei J, et al. Dominant clonotypes within HIV-specific T cell responses are programmed death-1high and CD127low and display reduced variant cross-reactivity. J Immunol. 2011;186:6871-85 pubmed publisher
	Monteiro P, Gosselin A, Wacleche V, El Far M, Said E, Kared H, et al. Memory CCR6+CD4+ T cells are preferential targets for productive HIV type 1 infection regardless of their expression of integrin ?7. J Immunol. 2011;186:4618-30 pubmed publisher
	Radziewicz H, Ibegbu C, Hon H, Bedard N, Bruneau J, Workowski K, et al. Transient CD86 expression on hepatitis C virus-specific CD8+ T cells in acute infection is linked to sufficient IL-2 signaling. J Immunol. 2010;184:2410-22 pubmed publisher
	Vojnov L, Reed J, Weisgrau K, Rakasz E, Loffredo J, Piaskowski S, et al. Effective simian immunodeficiency virus-specific CD8+ T cells lack an easily detectable, shared characteristic. J Virol. 2010;84:753-64 pubmed publisher
	Jones R, Yue F, Gu X, Hunter D, Mujib S, Gyenes G, et al. Human immunodeficiency virus type 1 escapes from interleukin-2-producing CD4+ T-cell responses without high-frequency fixation of mutations. J Virol. 2009;83:8722-32 pubmed publisher
	Chang W, Kim J, Kim Y, Kim Y, Lee J, Azuma M, et al. Cutting edge: Programmed death-1/programmed death ligand 1 interaction regulates the induction and maintenance of invariant NKT cell anergy. J Immunol. 2008;181:6707-10 pubmed
	Zahn R, Rett M, Korioth Schmitz B, Sun Y, Buzby A, Goldstein S, et al. Simian immunodeficiency virus (SIV)-specific CD8+ T-cell responses in vervet African green monkeys chronically infected with SIVagm. J Virol. 2008;82:11577-88 pubmed publisher

product information

Antigen :

CD279 (PD-1)

Apps. Abbrev. :

Flow Cytometry, Block, IHC

Cat # :

329912

Clone :

EH12.2H7

Item :

LEAF™ Purified anti-human CD279 (PD-1)

Isotype :

Mouse IgG1, κ

Other Names :

PD-1.

Size :

500 μg

Price (USD) :

375 USD

Reactivity :

Human, African Green, Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Rhesus, Squirrel Monkey

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Application Notes :

Additional reported applications (for the relevant formats) include: blocking of ligand binding 1-3 and immunohistochemical staining of paraformaldehyde fixed frozen sections 13 . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).