product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-human CD274 (B7-H1, PD-L1)
catalog :
329748
quantity :
100 mg
price :
3383 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
29E.2A3
reactivity :
human, mouse
application :
immunohistochemistry, neutralization, flow cytometry
more info or order :
citations: 21
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s1c
Morrissey M, Byrne R, Nulty C, McCabe N, Lynam Lennon N, Butler C, et al. The tumour microenvironment of the upper and lower gastrointestinal tract differentially influences dendritic cell maturation. BMC Cancer. 2020;20:566 pubmed publisher
  • flow cytometry; human; loading ...; fig 4e
Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
  • flow cytometry; human; loading ...; fig 5b, 5d, e4f
Mendoza J, Escalante N, Jude K, Sotolongo Bellon J, Su L, Horton T, et al. Structure of the IFNγ receptor complex guides design of biased agonists. Nature. 2019;567:56-60 pubmed publisher
  • flow cytometry; human; loading ...; fig s5a
Jung I, Kim Y, Yu H, Lee M, Kim S, Lee J. CRISPR/Cas9-Mediated Knockout of DGK Improves Antitumor Activities of Human T Cells. Cancer Res. 2018;78:4692-4703 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s1a
Luo N, Formisano L, Gonzalez Ericsson P, Sanchez V, Dean P, Opalenik S, et al. Melanoma response to anti-PD-L1 immunotherapy requires JAK1 signaling, but not JAK2. Oncoimmunology. 2018;7:e1438106 pubmed publisher
Nishi W, Wakamatsu E, Machiyama H, Matsushima R, Saito K, Yoshida Y, et al. Evaluation of therapeutic PD-1 antibodies by an advanced single-molecule imaging system detecting human PD-1 microclusters. Nat Commun. 2023;14:3157 pubmed publisher
Baleeiro R, Bouwens C, Liu P, DI Gioia C, Dunmall L, Nagano A, et al. MHC class II molecules on pancreatic cancer cells indicate a potential for neo-antigen-based immunotherapy. Oncoimmunology. 2022;11:2080329 pubmed publisher
Yang Z, Wang Y, Liu S, Deng W, Lomeli S, Moriceau G, et al. Enhancing PD-L1 Degradation by ITCH during MAPK Inhibitor Therapy Suppresses Acquired Resistance. Cancer Discov. 2022;12:1942-1959 pubmed publisher
Zhang Z, Yang A, Chaurasiya S, Park A, Lu J, Kim S, et al. CF33-hNIS-antiPDL1 virus primes pancreatic ductal adenocarcinoma for enhanced anti-PD-L1 therapy. Cancer Gene Ther. 2022;29:722-733 pubmed publisher
Meng Q, Xie S, Gray G, Dezfulian M, Li W, Huang L, et al. Empirical identification and validation of tumor-targeting T cell receptors from circulation using autologous pancreatic tumor organoids. J Immunother Cancer. 2021;9: pubmed publisher
Curnock A, Bossi G, Kumaran J, Bawden L, Figueiredo R, Tawar R, et al. Cell-targeted PD-1 agonists that mimic PD-L1 are potent T cell inhibitors. JCI Insight. 2021;6: pubmed publisher
Sekine T, Perez Potti A, Rivera Ballesteros O, Stralin K, Gorin J, Olsson A, et al. Robust T Cell Immunity in Convalescent Individuals with Asymptomatic or Mild COVID-19. Cell. 2020;183:158-168.e14 pubmed publisher
Wang H, Fu C, Du J, Wang H, He R, Yin X, et al. Enhanced histone H3 acetylation of the PD-L1 promoter via the COP1/c-Jun/HDAC3 axis is required for PD-L1 expression in drug-resistant cancer cells. J Exp Clin Cancer Res. 2020;39:29 pubmed publisher
RIEDER S, Wang J, White N, Qadri A, Ménard C, Stephens G, et al. B7-H7 (HHLA2) inhibits T-cell activation and proliferation in the presence of TCR and CD28 signaling. Cell Mol Immunol. 2021;18:1503-1511 pubmed publisher
Mancuso R, Casper J, Schmidt A, Krahenbuhl S, Weitz Schmidt G. Anti-αLβ2 antibodies reveal novel endocytotic cross-modulatory functionality. Br J Pharmacol. 2020;177:2696-2711 pubmed publisher
Grandclaudon M, Perrot Dockès M, Trichot C, Karpf L, Abouzid O, Chauvin C, et al. A Quantitative Multivariate Model of Human Dendritic Cell-T Helper Cell Communication. Cell. 2019;179:432-447.e21 pubmed publisher
Wang B, Zuo J, Kang W, Wei Q, Li J, Wang C, et al. Generation of Hutat2:Fc Knockin Primary Human Monocytes Using CRISPR/Cas9. Mol Ther Nucleic Acids. 2018;11:130-141 pubmed publisher
Chaganty B, Qiu S, Gest A, Lu Y, Ivan C, Calin G, et al. Trastuzumab upregulates PD-L1 as a potential mechanism of trastuzumab resistance through engagement of immune effector cells and stimulation of IFN? secretion. Cancer Lett. 2018;430:47-56 pubmed publisher
Burr M, Sparbier C, Chan Y, Williamson J, Woods K, Beavis P, et al. CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Nature. 2017;549:101-105 pubmed publisher
Weinberg A, Canniff J, Rouphael N, Mehta A, Mulligan M, Whitaker J, et al. Varicella-Zoster Virus-Specific Cellular Immune Responses to the Live Attenuated Zoster Vaccine in Young and Older Adults. J Immunol. 2017;199:604-612 pubmed publisher
Lu X, Horner J, Paul E, Shang X, Troncoso P, Deng P, et al. Effective combinatorial immunotherapy for castration-resistant prostate cancer. Nature. 2017;543:728-732 pubmed publisher
product information
Antigen :
CD274
Apps. Abbrev. :
FC, IHC, Block
Cat # :
329748
Clone :
29E.2A3
Item :
Ultra-LEAF™ Purified anti-human CD274 (B7-H1, PD-L1)
Isotype :
Mouse IgG2b, κ
Other Names :
Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)
Size :
100 mg
Price (USD) :
3383 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
ULEAF
Immunogen :
Full length human PD-L1
Application Notes :
Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region^5. Additional reported applications (for the relevant formats) include: blocking^1-3 and immunohistochemical staining of acetone-fixed frozen sections^1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748). It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.;Clone 29E.2A3 does not work in Western blot applications^7.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA