PerCP/Cyanine5.5 anti-human CD274 (B7-H1, PD-L1) | BioLegend 329737 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PerCP/Cyanine5.5 anti-human CD274 (B7-H1, PD-L1)

catalog :

329737

quantity :

25 tests

price :

159 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

29E.2A3

reactivity :

human

application :

flow cytometry, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; 5 ug/ml; loading ...; fig 2f, 3c flow cytometry; human; loading ...; fig 1b, 5c	Piao W, Li L, Saxena V, Iyyathurai J, Lakhan R, Zhang Y, et al. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration. Nat Commun. 2022;13:2176 pubmed publisher
	Diamantopoulos P, Kontandreopoulou C, Gkoufa A, Solomou E, Anastasopoulou A, Palli E, et al. Immunogenicity and Safety of the BNT162b2 mRNA COVID-19 Vaccine in Patients with Melanoma Treated with Immunotherapy. Cancers (Basel). 2022;14: pubmed publisher
	Wu Y, Shrestha P, Heape N, Yarchoan R. CDK4/6 inhibitors sensitize gammaherpesvirus-infected tumor cells to T-cell killing by enhancing expression of immune surface molecules. J Transl Med. 2022;20:217 pubmed publisher
	Duraiswamy J, Turrini R, Minasyan A, Barras D, Crespo I, Grimm A, et al. Myeloid antigen-presenting cell niches sustain antitumor T cells and license PD-1 blockade via CD28 costimulation. Cancer Cell. 2021;39:1623-1642.e20 pubmed publisher
	Zhang X, Peng L, Luo Y, Zhang S, Pu Y, Chen Y, et al. Dissecting esophageal squamous-cell carcinoma ecosystem by single-cell transcriptomic analysis. Nat Commun. 2021;12:5291 pubmed publisher
	Wang Y, Gu T, Tian X, Li W, Zhao R, Yang W, et al. A Small Molecule Antagonist of PD-1/PD-L1 Interactions Acts as an Immune Checkpoint Inhibitor for NSCLC and Melanoma Immunotherapy. Front Immunol. 2021;12:654463 pubmed publisher
	Ford B, Moncada Giraldo D, Margaroli C, Giacalone V, Brown M, Peng L, et al. Functional and Transcriptional Adaptations of Blood Monocytes Recruited to the Cystic Fibrosis Airway Microenvironment In Vitro. Int J Mol Sci. 2021;22: pubmed publisher
	Braun D, Street K, Burke K, Cookmeyer D, Denize T, Pedersen C, et al. Progressive immune dysfunction with advancing disease stage in renal cell carcinoma. Cancer Cell. 2021;39:632-648.e8 pubmed publisher
	Guo J, Muse E, Christians A, Swanson S, Davila E. An Anticancer Drug Cocktail of Three Kinase Inhibitors Improved Response to a Dendritic Cell-Based Cancer Vaccine. Cancer Immunol Res. 2019;7:1523-1534 pubmed publisher

product information

Antigen :

CD274

Apps. Abbrev. :

Cat # :

329737

Clone :

29E.2A3

Item :

PerCP/Cyanine5.5 anti-human CD274 (B7-H1, PD-L1)

Isotype :

Mouse IgG2b, κ

Other Names :

Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)

Size :

25 tests

Price (USD) :

159 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PERCP_CYANINE5.5

Immunogen :

Full length human PD-L1

Application Notes :

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region^5. Additional reported applications (for the relevant formats) include: blocking^1-3 and immunohistochemical staining of acetone-fixed frozen sections^1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748). It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.;Clone 29E.2A3 does not work in Western blot applications^7.;

more info or order :

BioLegend product webpage