Brilliant Violet 605™ anti-human CD274 (B7-H1, PD-L1) | BioLegend 329724 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 605™ anti-human CD274 (B7-H1, PD-L1)

catalog :

329724

quantity :

100 tests

price :

424 USD

clonality :

monoclonal

host :

mouse

conjugate :

BV605

clone name :

29E.2A3

reactivity :

human

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:100; loading ...; fig s20c	Mosaheb M, Dobrikova E, Brown M, Yang Y, Cable J, Okada H, et al. Genetically stable poliovirus vectors activate dendritic cells and prime antitumor CD8 T cell immunity. Nat Commun. 2020;11:524 pubmed publisher
	Lebel M, Coutelier M, Galipeau M, Kleinman C, Moon J, Melichar H. Differential expression of tissue-restricted antigens among mTEC is associated with distinct autoreactive T cell fates. Nat Commun. 2020;11:3734 pubmed publisher
	Mastoridis S, Bertolino G, Whitehouse G, Dazzi F, Sanchez Fueyo A, Martinez Llordella M. Multiparametric Analysis of Circulating Exosomes and Other Small Extracellular Vesicles by Advanced Imaging Flow Cytometry. Front Immunol. 2018;9:1583 pubmed publisher
	Maute R, Gordon S, Mayer A, McCracken M, Natarajan A, Ring N, et al. Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging. Proc Natl Acad Sci U S A. 2015;112:E6506-14 pubmed publisher

product information

Antigen :

CD274

Apps. Abbrev. :

Flow Cytometry

Cat # :

329724

Clone :

29E.2A3

Item :

Brilliant Violet 605™ anti-human CD274 (B7-H1, PD-L1)

Isotype :

Mouse IgG2b, κ

Other Names :

Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)

Size :

100 tests

Price (USD) :

424 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

BV605

Immunogen :

Full length human PD-L1

Application Notes :

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region 5 . Additional reported applications (for the relevant formats) include: blocking 1-3 and immunohistochemical staining of acetone-fixed frozen sections 1 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748). It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor 700 conjugate of interest. . Clone 29E.2A3 does not work in Western blot applications 7 .

more info or order :

BioLegend product webpage