PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1) | BioLegend 329718 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1)

catalog :

329718

quantity :

100 tests

price :

370 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

29E.2A3

reactivity :

human

application :

flow cytometry, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; 5 ug/ml; loading ...; fig 2f, 3c flow cytometry; human; loading ...; fig 1b, 5c	Piao W, Li L, Saxena V, Iyyathurai J, Lakhan R, Zhang Y, et al. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration. Nat Commun. 2022;13:2176 pubmed publisher
flow cytometry; human; loading ...; fig 6b	Rodriguez E, Boelaars K, Brown K, Eveline Li R, Kruijssen L, Bruijns S, et al. Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9. Nat Commun. 2021;12:1270 pubmed publisher
	Murayama T, Nakayama J, Jiang X, Miyata K, Morris A, Cai K, et al. Targeting DHX9 triggers tumor-intrinsic interferon response and replication stress in Small Cell Lung Cancer. Cancer Discov. 2024;: pubmed publisher
	Babl N, Hofbauer J, Matos C, Voll F, Menevse A, Rechenmacher M, et al. Low-density lipoprotein balances T cell metabolism and enhances response to anti-PD-1 blockade in a HCT116 spheroid model. Front Oncol. 2023;13:1107484 pubmed publisher
	Suszczyk D, Skiba W, Zardzewia x142 y W, Paw x142 owska A, W x142 odarczyk K, Polak G, et al. Clinical Value of the PD-1/PD-L1/PD-L2 Pathway in Patients Suffering from Endometriosis. Int J Mol Sci. 2022;23: pubmed publisher
	Yoshida R, Saigi M, Tani T, Springer B, Shibata H, Kitajima S, et al. MET-Induced CD73 Restrains STING-Mediated Immunogenicity of EGFR-Mutant Lung Cancer. Cancer Res. 2022;82:4079-4092 pubmed publisher
	Perdigoto A, Deng S, Du K, Kuchroo M, Burkhardt D, Tong A, et al. Immune cells and their inflammatory mediators modify β cells and cause checkpoint inhibitor-induced diabetes. JCI Insight. 2022;7: pubmed publisher
	Hu M, Alashkar Alhamwe B, Santner Nanan B, Miethe S, Harb H, Renz H, et al. Short-Chain Fatty Acids Augment Differentiation and Function of Human Induced Regulatory T Cells. Int J Mol Sci. 2022;23: pubmed publisher
	Zhang D, Reyes R, Osta E, Kari S, Gupta H, Padrón A, et al. Bladder cancer cell-intrinsic PD-L1 signals promote mTOR and autophagy activation that can be inhibited to improve cytotoxic chemotherapy. Cancer Med. 2021;10:2137-2152 pubmed publisher
	Shevyrev D, Tereshchenko V, Kozlov V, Sizikov A, Chumasova O, Koksharova V. T-regulatory cells from patients with rheumatoid arthritis retain suppressor functions in vitro. Exp Ther Med. 2021;21:209 pubmed publisher
	Chakraborty M, Chu K, Shrestha A, Revelo X, Zhang X, Gold M, et al. Mechanical Stiffness Controls Dendritic Cell Metabolism and Function. Cell Rep. 2021;34:108609 pubmed publisher
	Lv H, Lv G, Chen C, Zong Q, Jiang G, Ye D, et al. NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion. Cell Metab. 2021;33:110-127.e5 pubmed publisher
	Findlay E, Currie A, Zhang A, Ovciarikova J, Young L, Stevens H, et al. Exposure to the antimicrobial peptide LL-37 produces dendritic cells optimized for immunotherapy. Oncoimmunology. 2019;8:1608106 pubmed publisher
	Kitajima S, Ivanova E, Guo S, Yoshida R, Campisi M, Sundararaman S, et al. Suppression of STING Associated with LKB1 Loss in KRAS-Driven Lung Cancer. Cancer Discov. 2019;9:34-45 pubmed publisher
	Cañadas I, Thummalapalli R, Kim J, Kitajima S, Jenkins R, Christensen C, et al. Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses. Nat Med. 2018;24:1143-1150 pubmed publisher
	Zhao Y, Harrison D, Song Y, Ji J, Huang J, Hui E. Antigen-Presenting Cell-Intrinsic PD-1 Neutralizes PD-L1 in cis to Attenuate PD-1 Signaling in T Cells. Cell Rep. 2018;24:379-390.e6 pubmed publisher
	Zhang Q, Tian K, Xu J, Zhang H, Li L, Fu Q, et al. Synergistic Effects of Cabozantinib and EGFR-Specific CAR-NK-92 Cells in Renal Cell Carcinoma. J Immunol Res. 2017;2017:6915912 pubmed publisher
	Horn L, Ciavattone N, Atkinson R, Woldergerima N, Wolf J, Clements V, et al. CD3xPDL1 bi-specific T cell engager (BiTE) simultaneously activates T cells and NKT cells, kills PDL1+ tumor cells, and extends the survival of tumor-bearing humanized mice. Oncotarget. 2017;8:57964-57980 pubmed publisher
	Ezzelarab M, Raich Regue D, Lu L, Zahorchak A, Pérez Gutiérrez A, Humar A, et al. Renal Allograft Survival in Nonhuman Primates Infused With Donor Antigen-Pulsed Autologous Regulatory Dendritic Cells. Am J Transplant. 2017;17:1476-1489 pubmed publisher

product information

Antigen :

CD274

Apps. Abbrev. :

Cat # :

329718

Clone :

29E.2A3

Item :

PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1)

Isotype :

Mouse IgG2b, κ

Other Names :

Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)

Size :

100 tests

Price (USD) :

370 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PE_CYANINE7

Immunogen :

Full length human PD-L1

Application Notes :

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region^5. Additional reported applications (for the relevant formats) include: blocking^1-3 and immunohistochemical staining of acetone-fixed frozen sections^1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748). It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.;Clone 29E.2A3 does not work in Western blot applications^7.;

more info or order :

BioLegend product webpage