PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1) | BioLegend 329717 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1)

catalog :

329717

quantity :

25 tests

price :

176 USD

clonality :

monoclonal

host :

mouse

conjugate :

PE/Cy7

clone name :

29E.2A3

reactivity :

human

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 6h	Findlay E, Currie A, Zhang A, Ovciarikova J, Young L, Stevens H, et al. Exposure to the antimicrobial peptide LL-37 produces dendritic cells optimized for immunotherapy. Oncoimmunology. 2019;8:1608106 pubmed publisher
	Chakraborty M, Chu K, Shrestha A, Revelo X, Zhang X, Gold M, et al. Mechanical Stiffness Controls Dendritic Cell Metabolism and Function. Cell Rep. 2021;34:108609 pubmed publisher
	Lv H, Lv G, Chen C, Zong Q, Jiang G, Ye D, et al. NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion. Cell Metab. 2021;33:110-127.e5 pubmed publisher
	Deng H, Yang W, Zhou Z, Tian R, Lin L, Ma Y, et al. Targeted scavenging of extracellular ROS relieves suppressive immunogenic cell death. Nat Commun. 2020;11:4951 pubmed publisher
	Zietzer A, Hosen M, Wang H, Goody P, Sylvester M, Latz E, et al. The RNA-binding protein hnRNPU regulates the sorting of microRNA-30c-5p into large extracellular vesicles. J Extracell Vesicles. 2020;9:1786967 pubmed publisher
	Wang Z, Pascal L, Chandran U, Chaparala S, Lv S, Ding H, et al. ELL2 Is Required for the Growth and Survival of AR-Negative Prostate Cancer Cells. Cancer Manag Res. 2020;12:4411-4427 pubmed publisher
	Lee H, Oh Y, Jeon Y, Lee S, Kim H, Lee H, et al. DR4-Ser424 O-GlcNAcylation Promotes Sensitization of TRAIL-Tolerant Persisters and TRAIL-Resistant Cancer Cells to Death. Cancer Res. 2019;79:2839-2852 pubmed publisher
	Kitajima S, Ivanova E, Guo S, Yoshida R, Campisi M, Sundararaman S, et al. Suppression of STING Associated with LKB1 Loss in KRAS-Driven Lung Cancer. Cancer Discov. 2019;9:34-45 pubmed publisher
	Cañadas I, Thummalapalli R, Kim J, Kitajima S, Jenkins R, Christensen C, et al. Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses. Nat Med. 2018;24:1143-1150 pubmed publisher
	Zhao Y, Harrison D, Song Y, Ji J, Huang J, Hui E. Antigen-Presenting Cell-Intrinsic PD-1 Neutralizes PD-L1 in cis to Attenuate PD-1 Signaling in T Cells. Cell Rep. 2018;24:379-390.e6 pubmed publisher
	Zhang Q, Tian K, Xu J, Zhang H, Li L, Fu Q, et al. Synergistic Effects of Cabozantinib and EGFR-Specific CAR-NK-92 Cells in Renal Cell Carcinoma. J Immunol Res. 2017;2017:6915912 pubmed publisher
	Horn L, Ciavattone N, Atkinson R, Woldergerima N, Wolf J, Clements V, et al. CD3xPDL1 bi-specific T cell engager (BiTE) simultaneously activates T cells and NKT cells, kills PDL1+ tumor cells, and extends the survival of tumor-bearing humanized mice. Oncotarget. 2017;8:57964-57980 pubmed publisher
	Ezzelarab M, Raich Regue D, Lu L, Zahorchak A, Pérez Gutiérrez A, Humar A, et al. Renal Allograft Survival in Nonhuman Primates Infused With Donor Antigen-Pulsed Autologous Regulatory Dendritic Cells. Am J Transplant. 2017;17:1476-1489 pubmed publisher
	Maute R, Gordon S, Mayer A, McCracken M, Natarajan A, Ring N, et al. Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging. Proc Natl Acad Sci U S A. 2015;112:E6506-14 pubmed publisher

product information

Antigen :

CD274

Apps. Abbrev. :

Flow Cytometry

Cat # :

329717

Clone :

29E.2A3

Item :

PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1)

Isotype :

Mouse IgG2b, κ

Other Names :

Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)

Size :

25 tests

Price (USD) :

176 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PE_CYANINE7

Immunogen :

Full length human PD-L1

Application Notes :

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region 5 . Additional reported applications (for the relevant formats) include: blocking 1-3 and immunohistochemical staining of acetone-fixed frozen sections 1 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748). It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor 700 conjugate of interest. . Clone 29E.2A3 does not work in Western blot applications 7 .

more info or order :

BioLegend product webpage