Brilliant Violet 421™ anti-human CD274 (B7-H1, PD-L1) | BioLegend 329714 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-human CD274 (B7-H1, PD-L1)

catalog :

329714

quantity :

100 tests

price :

370 USD

clonality :

monoclonal

host :

mouse

conjugate :

BV421

clone name :

29E.2A3

reactivity :

human

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig s1c	Morrissey M, Byrne R, Nulty C, McCabe N, Lynam Lennon N, Butler C, et al. The tumour microenvironment of the upper and lower gastrointestinal tract differentially influences dendritic cell maturation. BMC Cancer. 2020;20:566 pubmed publisher
flow cytometry; human; loading ...; fig 5d	Roybal K, Williams J, Morsut L, Rupp L, Kolinko I, Choe J, et al. Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Cell. 2016;167:419-432.e16 pubmed publisher
	Ferrando Martinez S, Huang K, Bennett A, Sterba P, Yu L, Suzich J, et al. HBeAg seroconversion is associated with a more effective PD-L1 blockade during chronic hepatitis B infection. JHEP Rep. 2019;1:170-178 pubmed publisher
	Mansouri S, Katikaneni D, Gogoi H, Pipkin M, Machuca T, Emtiazjoo A, et al. Lung IFNAR1hi TNFR2+ cDC2 promotes lung regulatory T cells induction and maintains lung mucosal tolerance at steady state. Mucosal Immunol. 2020;13:595-608 pubmed publisher
	Chakravorty S, Yan B, Wang C, Wang L, Quaid J, Lin C, et al. Integrated Pan-Cancer Map of EBV-Associated Neoplasms Reveals Functional Host-Virus Interactions. Cancer Res. 2019;79:6010-6023 pubmed publisher
	Hunter S, Willcox C, Davey M, Kasatskaya S, Jeffery H, Chudakov D, et al. Human liver infiltrating γδ T cells are composed of clonally expanded circulating and tissue-resident populations. J Hepatol. 2018;69:654-665 pubmed publisher
	Horn L, Ciavattone N, Atkinson R, Woldergerima N, Wolf J, Clements V, et al. CD3xPDL1 bi-specific T cell engager (BiTE) simultaneously activates T cells and NKT cells, kills PDL1+ tumor cells, and extends the survival of tumor-bearing humanized mice. Oncotarget. 2017;8:57964-57980 pubmed publisher
	AlHossiny M, Luo L, Frazier W, Steiner N, Gusev Y, Kallakury B, et al. Ly6E/K Signaling to TGFÎ² Promotes Breast Cancer Progression, Immune Escape, and Drug Resistance. Cancer Res. 2016;76:3376-86 pubmed publisher
	Maute R, Gordon S, Mayer A, McCracken M, Natarajan A, Ring N, et al. Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging. Proc Natl Acad Sci U S A. 2015;112:E6506-14 pubmed publisher
	Nduom E, Wei J, Yaghi N, Huang N, Kong L, Gabrusiewicz K, et al. PD-L1 expression and prognostic impact in glioblastoma. Neuro Oncol. 2016;18:195-205 pubmed publisher

product information

Antigen :

CD274

Apps. Abbrev. :

Flow Cytometry

Cat # :

329714

Clone :

29E.2A3

Item :

Brilliant Violet 421™ anti-human CD274 (B7-H1, PD-L1)

Isotype :

Mouse IgG2b, κ

Other Names :

Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)

Size :

100 tests

Price (USD) :

370 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

BV421

Immunogen :

Full length human PD-L1

Application Notes :

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region 5 . Additional reported applications (for the relevant formats) include: blocking 1-3 and immunohistochemical staining of acetone-fixed frozen sections 1 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748). It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor 700 conjugate of interest. . Clone 29E.2A3 does not work in Western blot applications 7 .

more info or order :

BioLegend product webpage