Biotin anti-human CD274 (B7-H1, PD-L1) | BioLegend 329704 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Biotin anti-human CD274 (B7-H1, PD-L1)

catalog :

329704

quantity :

100 μg

price :

211 USD

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

29E.2A3

reactivity :

human

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 2

Reference
Yang Z, Wang Y, Liu S, Deng W, Lomeli S, Moriceau G, et al. Enhancing PD-L1 Degradation by ITCH during MAPK Inhibitor Therapy Suppresses Acquired Resistance. Cancer Discov. 2022;12:1942-1959 pubmed publisher
Hassounah N, Malladi V, Huang Y, Freeman S, Beauchamp E, Koyama S, et al. Identification and characterization of an alternative cancer-derived PD-L1 splice variant. Cancer Immunol Immunother. 2019;68:407-420 pubmed publisher

product information

Antigen :

CD274

Apps. Abbrev. :

Cat # :

329704

Clone :

29E.2A3

Item :

Biotin anti-human CD274 (B7-H1, PD-L1)

Isotype :

Mouse IgG2b, κ

Other Names :

Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)

Size :

100 μg

Price (USD) :

211 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

BIOTIN

Immunogen :

Full length human PD-L1

Application Notes :

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region^5. Additional reported applications (for the relevant formats) include: blocking^1-3 and immunohistochemical staining of acetone-fixed frozen sections^1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748). It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.;Clone 29E.2A3 does not work in Western blot applications^7.;

more info or order :

BioLegend product webpage