product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD244 (2B4)
catalog :
329502
quantity :
100 μg
price :
112 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
C1.7
reactivity :
human, mouse
application :
western blot, immunocytochemistry, flow cytometry, other, mass cytometry
more info or order :
citations: 22
Published Application/Species/Sample/DilutionReference
  • mass cytometry; human; loading ...; fig 2g
Weber E, Parker K, Sotillo E, Lynn R, Anbunathan H, Lattin J, et al. Transient rest restores functionality in exhausted CAR-T cells through epigenetic remodeling. Science. 2021;372: pubmed publisher
  • other; mouse
Tseng H, Xiong W, Badeti S, Yang Y, Ma M, Liu T, et al. Efficacy of anti-CD147 chimeric antigen receptors targeting hepatocellular carcinoma. Nat Commun. 2020;11:4810 pubmed publisher
  • flow cytometry; human; loading ...
Hood S, Cosma G, Foulds G, Johnson C, Reeder S, McArdle S, et al. Identifying prostate cancer and its clinical risk in asymptomatic men using machine learning of high dimensional peripheral blood flow cytometric natural killer cell subset phenotyping data. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig s3
Remmerswaal E, Hombrink P, Nota B, Pircher H, ten Berge I, van Lier R, et al. Expression of IL-7Rα and KLRG1 defines functionally distinct CD8+ T-cell populations in humans. Eur J Immunol. 2019;49:694-708 pubmed publisher
  • flow cytometry; human; loading ...; fig s2d
van Erp E, Feyaerts D, Duijst M, Mulder H, Wicht O, Luytjes W, et al. Respiratory Syncytial Virus Infects Primary Neonatal and Adult Natural Killer Cells and Affects Their Antiviral Effector Function. J Infect Dis. 2019;219:723-733 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Olin A, Henckel E, Chen Y, Lakshmikanth T, Pou C, Mikes J, et al. Stereotypic Immune System Development in Newborn Children. Cell. 2018;174:1277-1292.e14 pubmed publisher
  • mass cytometry; human; loading ...; fig 2a
Chew V, Lai L, Pan L, Lim C, Li J, Ong R, et al. Delineation of an immunosuppressive gradient in hepatocellular carcinoma using high-dimensional proteomic and transcriptomic analyses. Proc Natl Acad Sci U S A. 2017;114:E5900-E5909 pubmed publisher
  • flow cytometry; human; loading ...; fig 4h
Dias J, Leeansyah E, Sandberg J. Multiple layers of heterogeneity and subset diversity in human MAIT cell responses to distinct microorganisms and to innate cytokines. Proc Natl Acad Sci U S A. 2017;114:E5434-E5443 pubmed publisher
  • flow cytometry; human; loading ...; fig s8
Sen D, Kaminski J, Barnitz R, Kurachi M, Gerdemann U, Yates K, et al. The epigenetic landscape of T cell exhaustion. Science. 2016;354:1165-1169 pubmed
  • flow cytometry; human; loading ...; fig 1d
Yigit B, Halibozek P, Chen S, O Keeffe M, Arnason J, Avigan D, et al. A combination of an anti-SLAMF6 antibody and ibrutinib efficiently abrogates expansion of chronic lymphocytic leukemia cells. Oncotarget. 2016;7:26346-60 pubmed publisher
  • flow cytometry; human; fig 1
Schnorfeil F, Lichtenegger F, Emmerig K, Schlueter M, Neitz J, Draenert R, et al. T cells are functionally not impaired in AML: increased PD-1 expression is only seen at time of relapse and correlates with a shift towards the memory T cell compartment. J Hematol Oncol. 2015;8:93 pubmed publisher
  • flow cytometry; mouse; fig s1a
Chijioke O, Marcenaro E, Moretta A, Capaul R, Münz C. Role of the 2B4 Receptor in CD8+ T-Cell-Dependent Immune Control of Epstein-Barr Virus Infection in Mice With Reconstituted Human Immune System Components. J Infect Dis. 2015;212:803-7 pubmed publisher
  • flow cytometry; human; fig 2
Gütgemann S, Sandusky M, Wingert S, Claus M, Watzl C. Recruitment of activating NK-cell receptors 2B4 and NKG2D to membrane microdomains in mammalian cells is dependent on their transmembrane regions. Eur J Immunol. 2015;45:1258-69 pubmed publisher
  • immunocytochemistry; human
Chibueze C, Yoshimitsu M, Arima N. CD160 expression defines a uniquely exhausted subset of T lymphocytes in HTLV-1 infection. Biochem Biophys Res Commun. 2014;453:379-84 pubmed publisher
Lakshmikanth T, Muhammad S, Olin A, Chen Y, Mikes J, Fagerberg L, et al. Human Immune System Variation during 1 Year. Cell Rep. 2020;32:107923 pubmed publisher
Friebel E, Kapolou K, Unger S, Núñez N, Utz S, Rushing E, et al. Single-Cell Mapping of Human Brain Cancer Reveals Tumor-Specific Instruction of Tissue-Invading Leukocytes. Cell. 2020;181:1626-1642.e20 pubmed publisher
Zhuang X, Long E. CD28 Homolog Is a Strong Activator of Natural Killer Cells for Lysis of B7H7+ Tumor Cells. Cancer Immunol Res. 2019;7:939-951 pubmed publisher
Rölle A, Meyer M, Calderazzo S, Jager D, Momburg F. Distinct HLA-E Peptide Complexes Modify Antibody-Driven Effector Functions of Adaptive NK Cells. Cell Rep. 2018;24:1967-1976.e4 pubmed publisher
Bengsch B, Ohtani T, Khan O, Setty M, Manne S, O Brien S, et al. Epigenomic-Guided Mass Cytometry Profiling Reveals Disease-Specific Features of Exhausted CD8 T Cells. Immunity. 2018;48:1029-1045.e5 pubmed publisher
Rossi L, Avila D, Spallanzani R, Ziblat A, Fuertes M, Lapyckyj L, et al. Histone deacetylase inhibitors impair NK cell viability and effector functions through inhibition of activation and receptor expression. J Leukoc Biol. 2012;91:321-31 pubmed publisher
Correia D, Fogli M, Hudspeth K, da Silva M, Mavilio D, Silva Santos B. Differentiation of human peripheral blood V?1+ T cells expressing the natural cytotoxicity receptor NKp30 for recognition of lymphoid leukemia cells. Blood. 2011;118:992-1001 pubmed publisher
Hoffmann S, Cohnen A, Ludwig T, Watzl C. 2B4 engagement mediates rapid LFA-1 and actin-dependent NK cell adhesion to tumor cells as measured by single cell force spectroscopy. J Immunol. 2011;186:2757-64 pubmed publisher
product information
Antigen :
CD244
Apps. Abbrev. :
Flow Cytometry, Western Blotting
Cat # :
329502
Clone :
C1.7
Item :
Purified anti-human CD244 (2B4)
Isotype :
Mouse IgG1, κ
Other Names :
2B4, SLAMF4
Size :
100 μg
Price (USD) :
112 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Application Notes :
Additional reported applications (for the relevant formats) include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA