product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD326 (Ep-CAM)
catalog :
324202
quantity :
100 μg
price :
206 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
9C4
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 43
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig ex2a
Barkal A, Brewer R, Markovic M, Kowarsky M, Barkal S, Zaro B, et al. CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy. Nature. 2019;572:392-396 pubmed publisher
  • mass cytometry; human; loading ...; fig 1b
Wagner J, Rapsomaniki M, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer. Cell. 2019;177:1330-1345.e18 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig 9b
Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 2c
Peranzoni E, Lemoine J, Vimeux L, Feuillet V, Barrin S, Kantari Mimoun C, et al. Macrophages impede CD8 T cells from reaching tumor cells and limit the efficacy of anti-PD-1 treatment. Proc Natl Acad Sci U S A. 2018;115:E4041-E4050 pubmed publisher
  • flow cytometry; human; 1:25; loading ...; fig 3a
Britschgi A, Duss S, Kim S, Couto J, Brinkhaus H, Koren S, et al. The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERα. Nature. 2017;541:541-545 pubmed publisher
  • flow cytometry; human; fig 10c
Wands A, Fujita A, McCombs J, Cervin J, Dedic B, Rodriguez A, et al. Fucosylation and protein glycosylation create functional receptors for cholera toxin. elife. 2015;4:e09545 pubmed publisher
  • immunohistochemistry - frozen section; human; fig 1
  • flow cytometry; human; fig 1
Durrans A, Gao D, Gupta R, Fischer K, Choi H, El Rayes T, et al. Identification of Reprogrammed Myeloid Cell Transcriptomes in NSCLC. PLoS ONE. 2015;10:e0129123 pubmed publisher
  • flow cytometry; human; fig 6
Yang Y, Otte A, Hass R. Human mesenchymal stroma/stem cells exchange membrane proteins and alter functionality during interaction with different tumor cell lines. Stem Cells Dev. 2015;24:1205-22 pubmed publisher
  • flow cytometry; human; fig 4
Cai X, Dai Z, Reeves R, Caballero Benítez A, Duran K, Delrow J, et al. Autonomous stimulation of cancer cell plasticity by the human NKG2D lymphocyte receptor coexpressed with its ligands on cancer cells. PLoS ONE. 2014;9:e108942 pubmed publisher
  • western blot; human; 1:1000
Gao D, Vela I, Sboner A, Iaquinta P, Karthaus W, Gopalan A, et al. Organoid cultures derived from patients with advanced prostate cancer. Cell. 2014;159:176-187 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Gerlach K, Hwang Y, Nikolaev A, Atreya R, Dornhoff H, Steiner S, et al. TH9 cells that express the transcription factor PU.1 drive T cell-mediated colitis via IL-9 receptor signaling in intestinal epithelial cells. Nat Immunol. 2014;15:676-86 pubmed publisher
  • flow cytometry; human; fig 1c
Morris K, Khan H, Ahmad A, Weston L, Nofchissey R, Pinchuk I, et al. G-CSF and G-CSFR are highly expressed in human gastric and colon cancers and promote carcinoma cell proliferation and migration. Br J Cancer. 2014;110:1211-20 pubmed publisher
Qin X, Furutani Y, Yonezawa K, Shimizu N, Kato Murayama M, Shirouzu M, et al. Targeting transglutaminase 2 mediated exostosin glycosyltransferase 1 signaling in liver cancer stem cells with acyclic retinoid. Cell Death Dis. 2023;14:358 pubmed publisher
Yildizhan Y, Driessens K, Tsao H, Boiy R, Thomas D, Geukens N, et al. Detection of Breast Cancer-Specific Extracellular Vesicles with Fiber-Optic SPR Biosensor. Int J Mol Sci. 2023;24: pubmed publisher
Wang J, Dallmann R, Lu R, Yan J, Charmet J. Flow Rate-Independent Multiscale Liquid Biopsy for Precision Oncology. ACS Sens. 2023;8:1200-1210 pubmed publisher
Li G, Suzuki H, Ohishi T, Asano T, Tanaka T, Yanaka M, et al. Antitumor activities of a defucosylated anti‑EpCAM monoclonal antibody in colorectal carcinoma xenograft models. Int J Mol Med. 2023;51: pubmed publisher
Goh D, Lim J, Ferna xed ndez S, Joseph C, Edwards S, Neo Z, et al. Case report: Persistence of residual antigen and RNA of the SARS-CoV-2 virus in tissues of two patients with long COVID. Front Immunol. 2022;13:939989 pubmed publisher
Njock M, O Grady T, Nivelles O, Lion M, Jacques S, Cambier M, et al. Endothelial extracellular vesicles promote tumour growth by tumour-associated macrophage reprogramming. J Extracell Vesicles. 2022;11:e12228 pubmed publisher
Peterson H, Chin L, Iwamoto Y, Oh J, Carlson J, Lee H, et al. Integrated Analytical System for Clinical Single-Cell Analysis. Adv Sci (Weinh). 2022;9:e2200415 pubmed publisher
De Vargas Roditi L, Jacobs A, Rueschoff J, Bankhead P, Chevrier S, Jackson H, et al. Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer. Cell Rep Med. 2022;3:100604 pubmed publisher
Poggi A. Generation of Tumor Spheroids to Evaluate T Cell and NK Cell Cytotoxicity. Curr Protoc. 2022;2:e366 pubmed publisher
Shi G, Liu Y. Laboratory-Scale Production of Sterile Targeted Microbubbles. Methods Mol Biol. 2022;2394:591-599 pubmed publisher
Ramos S, Morton J, Yadav P, Reed B, Alizadeh S, Shilleh A, et al. Generation of functional human thymic cells from induced pluripotent stem cells. J Allergy Clin Immunol. 2022;149:767-781.e6 pubmed publisher
Leader A, Grout J, Maier B, Nabet B, Park M, Tabachnikova A, et al. Single-cell analysis of human non-small cell lung cancer lesions refines tumor classification and patient stratification. Cancer Cell. 2021;39:1594-1609.e12 pubmed publisher
Yildizhan Y, Vajrala V, Geeurickx E, Declerck C, Duskunovic N, De Sutter D, et al. FO-SPR biosensor calibrated with recombinant extracellular vesicles enables specific and sensitive detection directly in complex matrices. J Extracell Vesicles. 2021;10:e12059 pubmed publisher
Kaneko M, Ohishi T, Takei J, Sano M, Nakamura T, Hosono H, et al. Anti‑EpCAM monoclonal antibody exerts antitumor activity against oral squamous cell carcinomas. Oncol Rep. 2020;44:2517-2526 pubmed publisher
Morsing M, Kim J, Villadsen R, Goldhammer N, Jafari A, Kassem M, et al. Fibroblasts direct differentiation of human breast epithelial progenitors. Breast Cancer Res. 2020;22:102 pubmed publisher
Batth I, Meng Q, Wang Q, Torres K, Burks J, Wang J, et al. Rare osteosarcoma cell subpopulation protein array and profiling using imaging mass cytometry and bioinformatics analysis. BMC Cancer. 2020;20:715 pubmed publisher
Krishnan V, Lim D, Hoang P, Srivastava S, Matsuo J, Huang K, et al. DNA damage signalling as an anti-cancer barrier in gastric intestinal metaplasia. Gut. 2020;69:1738-1749 pubmed publisher
Davies S, Reynolds G, Wilkinson A, Li X, Rose R, Leekha M, et al. Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process. Cell Rep. 2019;29:1610-1620.e4 pubmed publisher
Valès S, Bacola G, Biraud M, Touvron M, Bessard A, Geraldo F, et al. Tumor cells hijack enteric glia to activate colon cancer stem cells and stimulate tumorigenesis. EBioMedicine. 2019;49:172-188 pubmed publisher
Ding L, Su Y, Fassl A, Hinohara K, Qiu X, Harper N, et al. Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ. Nat Commun. 2019;10:4182 pubmed publisher
Martin J, Chang C, Boschetti G, Ungaro R, Giri M, Grout J, et al. Single-Cell Analysis of Crohn's Disease Lesions Identifies a Pathogenic Cellular Module Associated with Resistance to Anti-TNF Therapy. Cell. 2019;178:1493-1508.e20 pubmed publisher
Salerno Goncalves R, Kayastha D, Fasano A, Levine M, Sztein M. Crosstalk between leukocytes triggers differential immune responses against Salmonella enterica serovars Typhi and Paratyphi. PLoS Negl Trop Dis. 2019;13:e0007650 pubmed publisher
Huerta Nuñez L, Gutiérrez Iglesias G, Martínez Cuazitl A, Mata Miranda M, Alvarez Jiménez V, Sánchez Monroy V, et al. A biosensor capable of identifying low quantities of breast cancer cells by electrical impedance spectroscopy. Sci Rep. 2019;9:6419 pubmed publisher
Genga R, Kernfeld E, Parsi K, Parsons T, Ziller M, Maehr R. Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development. Cell Rep. 2019;27:708-718.e10 pubmed publisher
Hinohara K, Wu H, Vigneau S, McDonald T, Igarashi K, Yamamoto K, et al. KDM5 Histone Demethylase Activity Links Cellular Transcriptomic Heterogeneity to Therapeutic Resistance. Cancer Cell. 2018;34:939-953.e9 pubmed publisher
Kim J, Villadsen R. Expression of Luminal Progenitor Marker CD117 in the Human Breast Gland. J Histochem Cytochem. 2018;66:879-888 pubmed publisher
Ruiz Riquelme A, Lau H, Stuart E, Goczi A, Wang Z, Schmitt Ulms G, et al. Prion-like propagation of β-amyloid aggregates in the absence of APP overexpression. Acta Neuropathol Commun. 2018;6:26 pubmed publisher
Hutt M, Fellermeier Kopf S, Seifert O, Schmitt L, Pfizenmaier K, Kontermann R. Targeting scFv-Fc-scTRAIL fusion proteins to tumor cells. Oncotarget. 2018;9:11322-11335 pubmed publisher
Gee M, Han A, Lofgren S, Beausang J, Mendoza J, Birnbaum M, et al. Antigen Identification for Orphan T Cell Receptors Expressed on Tumor-Infiltrating Lymphocytes. Cell. 2018;172:549-563.e16 pubmed publisher
Shultz L, Saito Y, Najima Y, Tanaka S, Ochi T, Tomizawa M, et al. Generation of functional human T-cell subsets with HLA-restricted immune responses in HLA class I expressing NOD/SCID/IL2r gamma(null) humanized mice. Proc Natl Acad Sci U S A. 2010;107:13022-7 pubmed publisher
product information
Antigen :
CD326
Apps. Abbrev. :
FC, ICC, IHC
Cat # :
324202
Clone :
9C4
Item :
Purified anti-human CD326 (Ep-CAM)
Isotype :
Mouse IgG2b, κ
Other Names :
Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Size :
100 μg
Price (USD) :
206 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
DU.4475 breast carcinoma
Application Notes :
Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry^3, and spatial biology (IBEX)^4,5.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA