Purified anti-human CD172a/b (SIRPα/β) | BioLegend 323802 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-human CD172a/b (SIRPα/β)

catalog :

323802

quantity :

100 μg

price :

108 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SE5A5

reactivity :

human

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 1e	Bujko A, Atlasy N, Landsverk O, Richter L, Yaqub S, Horneland R, et al. Transcriptional and functional profiling defines human small intestinal macrophage subsets. J Exp Med. 2018;215:441-458 pubmed publisher
flow cytometry; human; loading ...; fig s2a	Ring N, Herndler Brandstetter D, Weiskopf K, Shan L, Volkmer J, George B, et al. Anti-SIRPα antibody immunotherapy enhances neutrophil and macrophage antitumor activity. Proc Natl Acad Sci U S A. 2017;114:E10578-E10585 pubmed publisher
flow cytometry; human; fig 2f	See P, Dutertre C, Chen J, Günther P, McGovern N, Irac S, et al. Mapping the human DC lineage through the integration of high-dimensional techniques. Science. 2017;356: pubmed publisher
flow cytometry; human; fig 3	Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher
	Baskar R, Chen A, Favaro P, Reynolds W, Mueller F, Borges L, et al. Integrating transcription-factor abundance with chromatin accessibility in human erythroid lineage commitment. Cell Rep Methods. 2022;2: pubmed publisher
	Alcántara Hernández M, Idoyaga J. Mass cytometry profiling of human dendritic cells in blood and tissues. Nat Protoc. 2021;16:4855-4877 pubmed publisher
	Leylek R, Alcántara Hernández M, Granja J, Chavez M, Perez K, Diaz O, et al. Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity. Cell Rep. 2020;32:108180 pubmed publisher
	Tal M, Torrez Dulgeroff L, Myers L, Cham L, Mayer Barber K, Bohrer A, et al. Upregulation of CD47 Is a Host Checkpoint Response to Pathogen Recognition. MBio. 2020;11: pubmed publisher
	Martin J, Chang C, Boschetti G, Ungaro R, Giri M, Grout J, et al. Single-Cell Analysis of Crohn's Disease Lesions Identifies a Pathogenic Cellular Module Associated with Resistance to Anti-TNF Therapy. Cell. 2019;178:1493-1508.e20 pubmed publisher
	Jordan S, Tung N, Casanova Acebes M, Chang C, Cantoni C, Zhang D, et al. Dietary Intake Regulates the Circulating Inflammatory Monocyte Pool. Cell. 2019;178:1102-1114.e17 pubmed publisher
	Sim J, Sockolosky J, Sangalang E, Izquierdo S, Pedersen D, HARRIMAN W, et al. Discovery of high affinity, pan-allelic, and pan-mammalian reactive antibodies against the myeloid checkpoint receptor SIRPα. MAbs. 2019;11:1036-1052 pubmed publisher
	Sander J, Schmidt S, Cirovic B, McGovern N, Papantonopoulou O, Hardt A, et al. Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2. Immunity. 2017;47:1051-1066.e12 pubmed publisher
	Alcántara Hernández M, Leylek R, Wagar L, Engleman E, Keler T, Marinkovich M, et al. High-Dimensional Phenotypic Mapping of Human Dendritic Cells Reveals Interindividual Variation and Tissue Specialization. Immunity. 2017;47:1037-1050.e6 pubmed publisher
	Pilling D, Galvis Carvajal E, Karhadkar T, Cox N, Gomer R. Monocyte differentiation and macrophage priming are regulated differentially by pentraxins and their ligands. BMC Immunol. 2017;18:30 pubmed publisher
	Lopes F, BÃ¡lint Å, Valvo S, Felce J, Hessel E, Dustin M, et al. Membrane nanoclusters of FcÎ³RI segregate from inhibitory SIRPÎ± upon activation of human macrophages. J Cell Biol. 2017;216:1123-1141 pubmed publisher

product information

Antigen :

CD172a/b

Apps. Abbrev. :

Cat # :

323802

Clone :

SE5A5

Item :

Purified anti-human CD172a/b (SIRPα/β)

Isotype :

Mouse IgG1, κ

Other Names :

SIRPa, SIRPb, SIRPalpha/beta, BIT, SHPS1, MFR, P84, PTPNS1, CD172 antigen-like family member A, CD172 antigen-like family member B

Size :

100 μg

Price (USD) :

108 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PURE

Immunogen :

NIH-3T3/hu-SIRPα cell line

Application Notes :

Clone SE5A5 recognizes a common epitope on SIRPa (90 kD) and SIRPß (50 kD)^3. A high degree of homology has been found between SIRP family isoforms alpha and beta at the level of extracellular domains. Consequently, many anti SIRP antibody clones, such as SE5A5, have been reported to cross react with several SIRP isoforms^1,4,5. It reacts with CD172a and has weak cross-reaction with CD172b. This antibody is able to block the binding of SIRPa (SIRPa1 and SIRPa2) to CD47^1,6.;

more info or order :

BioLegend product webpage